Integrated Digital Microfluidic Platform for Colorimetric Sensing of Nitrite

Gu, Zhen; Wu, Minglei; Yan, Binbin; Wang, Huifeng; Kong, Cong

doi:10.1021/acsomega.0c01274

Cited by 29 publications

(15 citation statements)

References 35 publications

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“…The direction and velocity of droplet movements can be programmed according to the detection process even after the fabrication of the EWOD-based DMF chips. As various methods have been developed to fabricate DMF chips based on substrates such as silicon wafer, ITO glass, PCB board, and flexible polymers, the DMF technique is able to combine with a variety of analytical methods such as electrochemiluminescence [12], electrochemistry [13], and colorimetric sensing [14]. Currently, the EWOD-based DMF has been widely used in biosensing, environmental monitoring, and food safety, such as single-cell sequencing [2,15], cell invasion monitoring [5], detection of growth factors in embryo culture medium [9], bacterial classification [16], marine pollution monitoring [17], and detection of food nitrite [14].…”

Section: Introductionmentioning

confidence: 99%

Colorimetric Sensing with Gold Nanoparticles on Electrowetting-Based Digital Microfluidics

Luo

Ding

et al. 2021

Micromachines

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Digital microfluidic (DMF) has been a unique tool for manipulating micro-droplets with high flexibility and accuracy. To extend the application of DMF for automatic and in-site detection, it is promising to introduce colorimetric sensing based on gold nanoparticles (AuNPs), which have advantages including high sensitivity, label-free, biocompatibility, and easy surface modification. However, there is still a lack of studies for investigating the movement and stability of AuNPs for in-site detection on the electrowetting-based digital microfluidics. Herein, to demonstrate the ability of DMF for colorimetric sensing with AuNPs, we investigated the electrowetting property of the AuNPs droplets on the hydrophobic interface of the DMF chip and examined the stability of the AuNPs on DMF as well as the influence of evaporation to the colorimetric sensing. As a result, we found that the electrowetting of AuNPs fits to a modified Young–Lippmann equation, which suggests that a higher voltage is required to actuate AuNPs droplets compared with actuating water droplets. Moreover, the stability of AuNPs was maintained during the processing of electrowetting. We also proved that the evaporation of droplets has a limited influence on the detections that last several minutes. Finally, a model experiment for the detection of Hg2+ was carried out with similar results to the detections in bulk solution. The proposed method can be further extended to a wide range of AuNPs-based detection for label-free, automatic, and low-cost detection of small molecules, biomarkers, and metal ions.

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Section: Introductionmentioning

confidence: 99%

Colorimetric Sensing with Gold Nanoparticles on Electrowetting-Based Digital Microfluidics

Luo

Ding

et al. 2021

Micromachines

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“…DMF is different from channel microfluidics because it allows for individual sample droplet manipulation. [115] This device design in blood analysis includes the detection of exosomes that are released by tumor and nonmalignant cells and provides information on cell origins. [116] With the employment of microfluidic devices, sample treatment also revolves around the investigation of the physical and chemical properties of cells.…”

Section: Biomolecule Extraction Separation and Detection In Microchannelmentioning

confidence: 99%

Section: Application In Biomedical Engineering and Healthcarementioning

confidence: 99%

Micro/Nanofluidic‐Enabled Biomedical Devices: Integration of Structural Design and Manufacturing

Yin

Kim

2021

Advanced NanoBiomed Research

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Micro/nanofluidic devices and systems have attracted ever‐growing attention in healthcare applications over the past decades due to low‐cost yet easy‐customizable functions with the demand of only a small volume of sample fluid. The continuous development, in particular, supported by the emergence of new materials, capable of meeting critical needs in next‐generation, wearable, and multifunctional biomedical devices for at‐home, personalized healthcare monitoring, is challenging the principles and strategies of structural design, manufacturing, and their seamless integration. This review summarizes the progress in micro/nanofluidic‐enabled biomedical devices with a focus on structural design, manufacturing, and applications in healthcare. Structures of fluidic channels and liquid actuation strength are given to elucidate the manipulations and controls of fluid transports that help capture desirable information of interest, including component separation, extraction, measurements, and disease diagnoses. Manufacturing processes of fluidic devices in micro‐ and nanoscales and their basic working principles are also presented, ranging from lithography in traditional hard materials to 3D printing in emerging soft materials. The selected examples and demonstrations are illustrated to highlight applications of biomedical fluidic devices in a broad variety of disease detection and diagnosis. The associated challenges and future opportunities are discussed.

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“…31 An integrated digital microfluidic device based on the Griess assay is demonstrated for the detection of nitrite in food products. 32 Recently, a carbon fibre microelectrode is developed for the detection of nitrite in the brain of an anaesthetized rat. 33 Recently, electrochemical methods using modified electrodes employing nanoparticles and carbon nanotubes (CNTs) have been used for measuring nitrite.…”

Section: Introductionmentioning

confidence: 99%