Integrated elastomer-based device for measuring the mechanics of adherent cell monolayers

Abstract

We developed an integrated device for measuring the Young's modulus of adherent cell monolayers over time within a physiological environment.

In situ measurement of viscoelastic properties of cellular monolayers via graphene strain sensing of elastohydrodynamic phenomena

“…This suggests that MDCK cell bodies contribute more to the monolayer stiffness than the ECM and basement membrane proteins. Our results are in agreement with those by Sorba et al , 41 who showed that in MDCK monolayers, the cell covered region had a higher relaxation time constant (10.09 ± 1.42 s −1 ) than the bare PDMS region (3.18 ± 1.47 s −1 ), via tensile testing, and that EDTA treated MDCK cells had a lower Young's modulus (6.86 ± 3.27 kPa) than untreated cells (23.3 ± 6.3 kPa).…”

Mechanotransduction: Cellular dynamic mechanical behavior quantification and control

“…Then as a result, lower cellular Young's modulus were observed Teixeira et al (2003). Note that the Young's modulus of MDCK cells measured in this study 4-40 kPa is in agreement with previously reported values Sorba et al (2019). Furthermore, the lower contractility of the cells on the structured substrates leads to lower shear stress of the cells Mollaeian et al (2018b); .…”

An Open-Source Membrane Stretcher for Simultaneous Mechanical and Structural Characterizations of Soft Materials and Biological Tissues

“…There has also been development of open-source stretchers [13,19,18,20,24] to make the cell stretching systems more accessible. Recently, there have been developments of cell stretchers that also allow for force measurements [22]. For example, the deformation of a silicone sheet has been used to approximate the elastic module of a cell layer [22].…”