Integrated flow cytometric and proteomics analyses reveal the regulatory network underlying sugarcane protoplast responses to fusion

Wang, Rui; Li, Xinzhu; Zhu, Shuifang; Zhang, Demei; Han, Shijian; Li, Zhigang; Lu, Jiahui; Chu, Haiwei; Xiao, Jiming; Li, Suli

doi:10.1016/j.plaphy.2023.107918

Cited by 2 publications

(1 citation statement)

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“…Antagonistic experiments, molecular markers, colony morphology and other metrics and methods were used to identify whether fusants were successfully fused, and these methods could be combined and supported each other [38]. The mechanism of protoplast fusion is very complex [39]. The fusants recombine the genetic material of both parents, and antagonistic lines will be generated if the fusants are incompatible with both parents or between the fusion strains.…”

Section: Discussionmentioning

confidence: 99%

Interspecific Hybridization between Ganoderma lingzhi and G. resinaceum by PEG-Induced Double-Inactivated Protoplast Fusion

Li,

Liu,

et al. 2023

Horticulturae

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Ganoderma lingzhi is an important medicinal fungus, and it is particularly important to select strains with high yields and active substance contents. In this study, protoplasts of G. lingzhi were thermally inactivated to destroy intracellular enzyme proteins and preserve DNA. The DNA of G. resinaceum was damaged by ultraviolet (UV) radiation, and other components of the protoplasm except DNA were preserved. Then, the protoplast was induced using polyethylene glycol (PEG) for fusion. The results showed that the optimal thermal inactivation conditions for G. lingzhi were 30 min in a 45 °C water bath, and the optimal UV inactivation conditions for G. resinaceum were 70 s of irradiation using a 20 W UV lamp at a vertical distance of 15 cm. Antagonistic tests, internal transcribed space (ITS) and mitochondrial DNA identification, intersimple sequence repeat (ISSR) molecular markers and morphology were used to distinguish the parents from the fusants. Four true fusants were obtained, and the yield was 2.5%. The fruiting body yield of the fusants was significantly higher than that of G. lingzhi, and the polysaccharide and triterpene contents of the RAD-64 fusant were significantly higher than those of G. lingzhi. The results presented in this paper show that protoplast fusion technology can effectively improve G. lingzhi varieties and support the breeding of new varieties.

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Section: Discussionmentioning

confidence: 99%