2018
DOI: 10.1016/j.jim.2017.11.010
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Integrated functional and mass spectrometry-based flow cytometric phenotyping to describe the immune microenvironment in acute myeloid leukemia

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Cited by 13 publications
(8 citation statements)
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References 63 publications
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“…Immunophenotyping by CyTOF. Samples were stained for surface and intracellular molecules following protocols from Fluidigm, as detailed in SI Appendix, Supplemental Materials and Methods and as published in Lamble et al (77), and analyzed on a CyTOF 1 instrument. Two separate panels of antibodies were used for phenotyping (SI Appendix, Table S4).…”
Section: Methodsmentioning
confidence: 99%
“…Immunophenotyping by CyTOF. Samples were stained for surface and intracellular molecules following protocols from Fluidigm, as detailed in SI Appendix, Supplemental Materials and Methods and as published in Lamble et al (77), and analyzed on a CyTOF 1 instrument. Two separate panels of antibodies were used for phenotyping (SI Appendix, Table S4).…”
Section: Methodsmentioning
confidence: 99%
“…The CyTOF data were analyzed on Cytobank 1 . Cell types were identified based on the following parameters: T cells, CD45+CD3+; B cells, CD45+CD19+; natural killer (NK) cells, CD45+CD3-CD16+CD56+ (19); monocytes, CD45+CD14+CD16+ (20); macrophages or microglia cells, CD45+CD11b+CD68+ (21); Tregs, CD45+CD4+CD25+CD127- (22); naïve CD4+ T cells, CD45+CD45RA+CCR7+CD4+ (23); and naïve CD8+ T cells, CD45+CD45RA+CCR7+CD8+ (23). Monocytes and macrophages constitute mononuclear phagocytes (24) (Supplementary Table S2).…”
Section: Cytof Data Analysismentioning
confidence: 99%
“…cytobank.org). The cell types were identified based on the following parameters: T cells, CD45+ CD3+; natural killer (NK) cells, CD45+ CD3-CD16+ CD56+ (10,19); B cells, CD45+ CD19+; monocytes, CD45+ CD14+ CD16+ 20; macrophages or microglial cells, CD45+ CD11b+ CD3-CD19-CD66b- (15); Tregs, CD45+ CD4+ CD25+ CD127- (21), and granulocytes, CD45+ CD66b+. Monocytes and macrophages constitute mononuclear phagocytes (22).…”
Section: Cytof Data Analysismentioning
confidence: 99%