Digital in-line holography is an optical technique which can be applied to measure the size, three-dimensional position, and three-component velocity of disperse particle fields. This work summarizes recent developments at Sandia National Laboratories focused on improvement in measurement accuracy, experimental validation, and applications to multiphase flows. New routines are presented which reduce the uncertainty in measured position along the optical axis to a fraction of the particle diameter. Furthermore, application to liquid atomization highlights the ability to measure complex, three-dimensional structures. Finally, investigation of particles traveling at near sonic conditions prove accuracy despite significant experimental noise due to shock-waves.4