Integrated gut microbiome and metabolome analysis reveals the inhibition effect of <i>Lactobacillus plantarum</i> CBT against colorectal cancer

Chen, Yan-Yan; Fei, Fei; Ding, Ling-Ling; Wen, Shi-Yuan; Ren, Cai-Fang; Gong, Ai-Hua

doi:10.1039/d3fo04806c

Cited by 7 publications

(5 citation statements)

References 33 publications

(45 reference statements)

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“…Moreover, butyrate was shown to inhibit gastric tumors by reducing the expression of immunosuppressive factors, such as PD-L1 and IL-10 ( 87 ). In the context of colorectal cancer, a recent study demonstrated that oral administration of Lactobacillus plantarum CBT could effectively inhibit the growth of colorectal cancer in noth orthotopic as well as ectopic preclinical mouse models ( 88 ). Given the evidence that MH could effect changes in gut microbiota content, it would be extremely beneficial to characterize the relative changes in the metabolite abundance with a view of uncovering their influence on cancer growth and response to therapy.…”

Section: Discussionmentioning

confidence: 99%

Oral administration of Manuka honey induces IFNγ-dependent resistance to tumor growth that correlates with beneficial modulation of gut microbiota composition

Masad,

Idriss,

Mohamed

et al. 2024

Front. Immunol.

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BackgroundTo investigate the potential of Manuka honey (MH) as an immunomodulatory agent in colorectal cancer (CRC) and dissect the underlying molecular and cellular mechanisms.MethodsMH was administered orally over a 4 week-period. The effect of MH treatment on microbiota composition was studied using 16S rRNA sequencing of fecal pellets collected before and after treatment. Pretreated mice were implanted with CRC cells and followed for tumor growth. Tumors and lymphoid organs were analyzed by flow cytometry (FACS), immunohistochemistry and qRT-PCR. Efficacy of MH was also assessed in a therapeutic setting, with oral treatment initiated after tumor implantation. We utilized IFNγ-deficient mice to determine the importance of interferon signaling in MH-induced immunomodulation.ResultsPretreatment with MH enhanced anti-tumor responses leading to suppression of tumor growth. Evidence for enhanced tumor immunogenicity included upregulated MHC class-II on intratumoral macrophages, enhanced MHC class-I expression on tumor cells and increased infiltration of effector T cells into the tumor microenvironment. Importantly, oral MH was also effective in retarding tumor growth when given therapeutically. Transcriptomic analysis of tumor tissue highlighted changes in the expression of various chemokines and inflammatory cytokines that drive the observed changes in tumor immunogenicity. The immunomodulatory capacity of MH was abrogated in IFNγ-deficient mice. Finally, bacterial 16S rRNA sequencing demonstrated that oral MH treatment induced unique changes in gut microbiota that may well underlie the IFN-dependent enhancement in tumor immunogenicity.ConclusionOur findings highlight the immunostimulatory properties of MH and demonstrate its potential utilization in cancer prevention and treatment.

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Section: Discussionmentioning

confidence: 99%

Oral administration of Manuka honey induces IFNγ-dependent resistance to tumor growth that correlates with beneficial modulation of gut microbiota composition

Masad,

Idriss,

Mohamed

et al. 2024

Front. Immunol.

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“…Lactobacillus , known for its positive influence on colonic barrier function, prevents cytokine-induced apoptosis of intestinal epithelial cells and increases Muc2 mRNA levels in vitro . Several studies have reported that interventions with probiotics, prebiotics, and postbiotics can significantly increase the abundance of Lactobacillus in the gut microbiota of mice. − A high proportion of Bacteroidetes is associated with colitis resistance and low-grade systemic inflammation . Previous research has demonstrated that Enterorhabdus, which exhibited a negative correlation with pro-inflammatory cytokine levels, contributed positively to intestinal homeostasis.…”

Section: Discussionmentioning

confidence: 99%

Bifidobacterium bifidum Ameliorates DSS-Induced Colitis in Mice by Regulating Microbial Metabolome and Targeting Gut Microbiota

Han,

Liang,

Hou

et al. 2024

J. Agric. Food Chem.

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Inflammatory bowel disease (IBD) is a recurrent inflammatory condition affecting the gastrointestinal tract, and its clinical treatment remains suboptimal. Probiotics have shown effectiveness in alleviating dextran sulfate sodium salt (DSS)-induced colitis, exhibiting strain-specific anti-inflammatory properties. In this study, we compared the therapeutic effects of five strains of Bifidobacterium bifidum isolated from healthy adult feces on DSS-induced colitis in mice. Additionally, we investigated the underlying mechanisms by examining gut microbiota composition and microbial metabolome. Our findings highlighted the superior efficacy of B. bifidum M1-3 compared to other strains. It significantly improved colitis symptoms, mitigated gut barrier disruption, and reduced colonic inflammation in DSS-treated mice. Moreover, gut microbiota composition analysis revealed that B. bifidum M1-3 treatment increased the abundance and diversity of gut microbiota. Specifically, it significantly increased the abundance of Muribaculaceae, Lactobacillus, Bacteroides, and Enterorhabdus, while decreasing the abundance of Escherichia-Shigella. Furthermore, our nontargeted metabolomics analysis illustrated that B. bifidum M1-3 treatment had a regulatory effect on various metabolic pathways, including tyrosine metabolism, lysine degradation, and tryptophan metabolism. Importantly, we confirmed that the therapeutic efficiency of B. bifidum M1-3 was dependent on the gut microbiota. These results are conducive to the development of probiotic products for alleviating colitis.

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“…Following sample homogenization, the filtrate was detected with the Thermo Vanquish (VQ-CORE-ISO-01, Thermo Fisher Scientific, USA) ultrahigh performance liquid chromatography system and the Thermo Orbitrap Exploris 120 mass spectrometry detector (BRE725531, Thermo Fisher Scientific, USA) equipped with an electrospray ion source (ESI). The detailed procedures can be found in the work by Chen et al in 2024 …”

Section: Methodsmentioning

confidence: 99%

“…The detailed procedures can be found in the work by Chen et al in 2024. 18 Statistical Analysis. All the statistical analyses were conducted using GraphPad Prism version 8.0.1.…”

Section: ■ Introductionmentioning

confidence: 99%

Lactobacillus plantarum-Derived Extracellular Vesicles Modulate Macrophage Polarization and Gut Homeostasis for Alleviating Ulcerative Colitis

Chen,

Fang,

Yang

et al. 2024

J. Agric. Food Chem.

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Extracellular vesicles released by probiotics have been demonstrated to effectively alleviate intestinal inflammation, yet the precise underlying mechanisms remain unclear. In this research, for the first time, Lactobacillus plantarum UJS001 (LP-UJS) was isolated from fermented sauerkraut in Zhenjiang, China. Thereafter, the therapeutic effect of LP-UJS-derived extracellular vesicles (LP-UJS-EVs) on dextran sulfate sodium-induced ulcerative colitis (UC) in mice was analyzed to elucidate the immune mechanisms. According to our findings, LP-UJS-EVs played a pivotal role in restoring the intestinal barrier and alleviating intestinal inflammation. Notably, LP-UJS-EVs induced M2 polarization of macrophages, promoted the release of IL-10 and TGF-β, inhibited the release of histamine, IL-6, and TNF-α, and exerted regulatory effects on intestinal microflora, as evidenced by the reduced abundances of Coprococcus, Parabacteroides, Staphylococcus, and Allobaculum, alongside the enhanced abundance of Prevotella. Furthermore, both LP-UJS and LP-UJS-EVs affected the lysine degradation pathway and significantly increased the abundance of related metabolites, especially oxoadipic acid. In summary, our results underscore the substantial therapeutic potential of LP-UJS and its secreted EVs in the treatment of UC.

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Integrated gut microbiome and metabolome analysis reveals the inhibition effect of Lactobacillus plantarum CBT against colorectal cancer

Abstract: The microecological stability of the gut microbiota plays a pivotal role in both preventing and treating colorectal cancer (CRC). This study investigated whether Lactobacillus plantarum CBT (LP-CBT) prevents CRC by...

Cited by 7 publications

References 33 publications

Oral administration of Manuka honey induces IFNγ-dependent resistance to tumor growth that correlates with beneficial modulation of gut microbiota composition

Oral administration of Manuka honey induces IFNγ-dependent resistance to tumor growth that correlates with beneficial modulation of gut microbiota composition

Bifidobacterium bifidum Ameliorates DSS-Induced Colitis in Mice by Regulating Microbial Metabolome and Targeting Gut Microbiota

Lactobacillus plantarum-Derived Extracellular Vesicles Modulate Macrophage Polarization and Gut Homeostasis for Alleviating Ulcerative Colitis

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