Integrated luminometer for the determination of trace metals in seawater using fluorescence, phosphorescence and chemiluminescence detection

Worsfold, Paul J.; Achterberg, Eric P.; Cannizzaro, V.; Charles, S.; Costa, José Manuel; Dubois, Fabien; Pereiro, Rosario; Vicente, Blanca San; Sanz‐Medel, Alfredo; Vandeloise, R.; Donckt, E. Vander; Wollast, P.; Yunus, Sami

doi:10.1155/s1463924602000081

Cited by 11 publications

(3 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, most of the flow-based analysers proposed in the literature are dedicated systems focusing on the determination of one or two parameters in water samples [ 5 – 8 ], mainly for oceanographic research. Less frequent are those presenting the development of a multiparametric analyser [ 9 ]. Recently, the concept of a computer supported system of networked automated water analysers has been introduced, the European AWACSS system [ 10 , 11 ] being an example of a continent-wide network of analysers.…”

Section: Introductionmentioning

confidence: 99%

Development of a Low-Cost SIA-Based Analyser for Water Samples

Knochen

Caamaño

Bentos

2011

Journal of Automated Methods and Management in Chemistry

View full text Add to dashboard Cite

An automated multiparametric water analyser was developed and evaluated. The system was based on Sequential Injection Analysis and featured a photometric detection system comprising a tricolour RGB LED source and a photodiode. A program compiled in Visual Basic was used to control the SIA flow system, the LEDs, and the data acquisition and processing. The program loads and executes methods written in ASCII and stored as text files. The system was capable of handling up to four methods simultaneously. When used to carry out methods based on the APHA standard methods, the figures of merit obtained were considered satisfactory for the purpose. The total cost was under US $4600. It was concluded that the analyser is appropriate for routine use and has potential for an increased number of simultaneous methods and for enhanced capabilities if new versions of the software are developed.

show abstract

Section: Introductionmentioning

confidence: 99%

Development of a Low-Cost SIA-Based Analyser for Water Samples

Knochen

Caamaño

Bentos

2011

Journal of Automated Methods and Management in Chemistry

View full text Add to dashboard Cite

show abstract

“…The fast and transient nature of solution chemiluminescence reactions requires rapid and reproducible sample and reagent mixing, and FIA provides the ideal means of performing these fluidic manipulations [56,57]. For example, the luminol reaction is commonly used as the basis for the chemiluminescent determination of trace metals and hydrogen peroxide in seawater [58,59].…”

Section: Luminescence Detectionmentioning

confidence: 99%

Flow injection analysis as a tool for enhancing oceanographic nutrient measurements—A review

Worsfold

Clough

Lohan

et al. 2013

Analytica Chimica Acta

View full text Add to dashboard Cite

“…There are several published FI methods for the determination of Zn 2+ in seawater samples which rely on a preconcentration step to reach nanomolar sensitivity and eliminate interferences from the seawater matrix. [10][11][12] Of these, the method of Nowicki et al 10 is the only shipboard FIA technique with adequate sensitivity for the analysis of Zn 2+ at open ocean seawater concentrations.…”

Section: Introductionmentioning

confidence: 99%

Determination of dissolved zinc in seawater using micro-Sequential Injection lab-on-valve with fluorescence detection

Grand

Oliveira

Růžička

et al. 2011

Analyst

View full text Add to dashboard Cite

This paper introduces the preliminary design and optimization of a micro-Sequential Injection lab-on-valve system (μSI-LOV) with fluorescence detection for the direct determination of trace Zn(2+) in an unacidified seawater matrix. The method capitalizes on the sensitivity and selectivity of FluoZin-3, which was originally designed to measure zinc in living cells. The optimum reaction conditions, sources of blank signal and physical parameters of the μSIA-LOV are evaluated with the requirements of trace metal analysis in mind, namely high sensitivity and low background signals. A detailed investigation of the effect of sample and reagent sequencing on sensitivity is presented for the first time using μSIA-LOV. We find that the order of sequencing greatly influences peak shape and analytical sensitivity with the highest and smoothest peaks obtained when a large volume of sample (75 μL) is aspirated last in the sequence prior to flow reversal and detection. The optimized reaction conditions and reagent/sample sequencing protocol yield a detection limit of 0.3 nM Zn(2+), high precision (RSD < 2.5%), a linear quantification range up to 40 nM and an analytical cycle of ∼1 min per sample. This work demonstrates that μSI-LOV is capable of attaining detection limits that are close to those needed for open ocean determinations of Zn(2+) without preconcentration or separation of the analyte from the seawater matrix. The low reagent consumption (50 μL per sample), full automation and minimal maintenance requirements of μSI-LOV make it well suited for shipboard analysis and, eventually, for development to meet the pressing need for trace element measurements in unattended locations.

show abstract

Integrated luminometer for the determination of trace metals in seawater using fluorescence, phosphorescence and chemiluminescence detection

Cited by 11 publications

References 7 publications

Development of a Low-Cost SIA-Based Analyser for Water Samples

Development of a Low-Cost SIA-Based Analyser for Water Samples

Flow injection analysis as a tool for enhancing oceanographic nutrient measurements—A review

Determination of dissolved zinc in seawater using micro-Sequential Injection lab-on-valve with fluorescence detection

Contact Info

Product

Resources

About