Integrated management of the Fusarium vascular wilt disease of Cucurbita pepo in Iraq

Abstract

Fusarium vascular wilt disease is one of the most harmful disease that affected broad range of plant species including zucchini (Cucurbita pepo). The objective of the research was to investigate the presence of the phytopathogenic gungus Fusarium oxysporum the causal agent of the disease in zucchini fields. Forty five isolates of F. oxysporum were isolated from four locations in province of Dyala during 2014-2015. Isolate Foq9 was most virulent in the pathogenicity test in vitro. Twenty nine isolates of them amplified their DNA positively with the universal primer of F. oxysporum in the polymerase chain reaction technique (PCR). In vitro significant suppression efficiency were observed of the fungicides Topsin M 70 WP (Tm) and Tecto 500 SC (Tc) and two botanical extract solution extracted from Garlic (Gr) and Ginger (Gn) against the pathogen on the potato sucrose agar (PSA). In greenhouse experiments all of the agents decreased the percentage of disease incidence and severity significantly, while the tetra-inoculum (Tm+Tc+Gr+Gn) was superior which exhibited 0% disease incidence and severity compared to the negative control which was 95%, 79% respectively, also the combined application of the botanical extracts (Gr+Gn) reduced the disease incidence and severity significantly. All of the treatment increased plant growth criteria represented by dry weight of the plant compared to the control.

Biocontrol of Rhizoctonia Solani in Cucumber Plants Using Endobacteria: Effects On Disease Severity and Chlorophyll Content

“…The cucumber plant belongs to the cucurbit family, Cucurabitaceae, and it is one of diffusively vegetable crops in the world [1]. Many fungal pathogens affect cucumber plants, the most important of which are Pythium aphanidermatum, Rhizoctonia solani, Fusarium oxysporum, and Macrophomina phaseolina [2,3,4]. Rhizoctonia solani is a soil fungus that is diffusively in regions of the world, causing great losses, as it can attack plant seeds and seedlings as well as crop roots of different plant hosts [5].…”