Integrated metabolic profiling and transcriptome analysis of pigment accumulation in diverse petal tissues in the lily cultivar ‘Vivian’

Yin, Xiaojuan; Lin, Xinyue; Liu, Yuxuan; Irfan, Muhammad; Chen, Lijing; Zhang, Li

doi:10.1186/s12870-020-02658-z

Cited by 15 publications

(14 citation statements)

References 90 publications

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“…In this study, we characterized LvMYB5, a member of the SG6 subgroup of MYB positive regulators that activate the anthocyanin biosynthesis pathway. The C-terminal sequence in LvMYB5 differs from those in other MYB proteins ( Supplementary Figure 4 ), which may be due to species-specific differences ( Yin et al, 2020 ). Recently, R3-MYB inhibitors have also been reported from lily ( Sakai et al, 2019 ), and these inhibitors may be derived from n-terminal truncation of SG4 subgroup MYB proteins ( LaFountain and Yuan, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

“…The coordinated expression of genes that encode enzymes in the flavonoid biosynthesis pathway, also called structural genes, including chalcone synthase ( CHS ), chalcone isomerase ( CHI ), flavanone 3-hydroxylase ( F3H ), flavonoid 3′-hydroxylase ( F3′H ), dihydroflavonol-4-reductase ( DFR ), and anthocyanidin synthase ( ANS ), as well as UDP-glucose:flavonoid-3-O-glucosyltransferase ( 3GT ), can directly affect the biosynthesis of anthocyanins, and thus affect flower color ( Nishihara and Nakatsuka, 2011 ; Yin et al, 2021 ). It has been shown that flower color formation is closely related to expression of the late structural genes DFR, ANS , and 3GT , which may directly affect flower color formation in lily ( Yin et al, 2020 ). Among these genes, ANS , which encodes anthocyanidin synthase, is an important enzyme that converts colorless leucoanthocyanidins into colored anthocyanidins ( Jaakola, 2013 ; Li et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

“…In particular, the negative regulators of anthocyanin biosynthesis are less studied compared to TFs that positively regulate floral pigmentation. Yin et al (2020) carried out a detailed analysis of lily petal RNA-seq (PRJNA649743) data and characterized genes related to the regulation of lily petal coloring, including structural genes and MYB transcription factor genes. The FPKM value of LvMYB5 (TRINITY_DN103447_c0_g1) was increased during lily flower coloration, and was possibly promoting the coloring of lily flowers.…”

Section: Introductionmentioning

confidence: 99%

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Regulation of MYB Transcription Factors of Anthocyanin Synthesis in Lily Flowers

Yin

Zhang

et al. 2021

Front. Plant Sci.

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Flower color is the decisive factor that affects the commercial value of ornamental flowers. Therefore, it is important to study the regulation of flower color formation in lily to discover the positive and negative factors that regulate this important trait. In this study, MYB transcription factors (TFs) were characterized to understand the regulatory mechanism of anthocyanin biosynthesis in lily. Two R2R3-MYB TFs, LvMYB5, and LvMYB1, were found to regulate anthocyanin biosynthesis in lily flowers. LvMYB5, which has an activation motif, belongs to the SG6 MYB protein subgroup of Arabidopsis thaliana. Transient expression of LvMYB5 indicated that LvMYB5 can promote coloration in Nicotiana benthamiana leaves, and that expression of LvMYB5 increases the expression levels of NbCHS, NbDFR, and NbANS. VIGS experiments in lily petals showed that the accumulation of anthocyanins was reduced when LvMYB5 was silenced. Luciferase assays showed that LvMYB5 can promote anthocyanin synthesis by activating the ANS gene promoter. Therefore, LvMYB5 plays an important role in flower coloration in lily. In addition, the transient expression experiment provided preliminary evidence that LvMYB1 (an R2R3-MYB TF) inhibits anthocyanin synthesis in lily flowers. The discovery of activating and inhibitory factors related to anthocyanin biosynthesis in lily provides a theoretical basis for improving flower color through genetic engineering. The results of our study provide a new direction for the further study of the mechanisms of flower color formation in lilies.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Regulation of MYB Transcription Factors of Anthocyanin Synthesis in Lily Flowers

Yin

Zhang

et al. 2021

Front. Plant Sci.

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“…The relative expression level was determined according to Sun et al (2020). The 2 − Ct methods were used to calculate the relative expression level of AnDHN (Yin et al, 2020).…”

Section: Real-time-qpcrmentioning

confidence: 99%

AnDHN, a Dehydrin Protein From Ammopiptanthus nanus, Mitigates the Negative Effects of Drought Stress in Plants

et al. 2021

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Dehydrins (DHNs) play crucial roles in a broad spectrum of abiotic stresses in model plants. However, the evolutionary role of DHNs has not been explored, and the function of DHN proteins is largely unknown in Ammopiptanthus nanus (A. nanus), an ancient and endangered legume species from the deserts of northwestern China. In this study, we isolated a drought-response gene (c195333_g1_i1) from a drought-induced RNA-seq library of A. nanus. Evolutionary bioinformatics showed that c195333_g1_i1 is an ortholog of Arabidopsis DHN, and we renamed it AnDHN. Moreover, DHN proteins may define a class of proteins that are evolutionarily conserved in all angiosperms that have experienced a contraction during the evolution of legumes. Arabidopsis plants overexpressing AnDHN exhibited morpho-physiological changes, such as an increased germination rate, higher relative water content (RWC), higher proline (PRO) content, increased peroxidase (POD) and catalase (CAT) activities, lower contents of malondialdehyde (MDA), H2O2 and O2–, and longer root length. Our results showed that the transgenic lines had improved drought resistance with deep root system architecture, excellent water retention, increased osmotic adjustment, and enhanced reactive oxygen species (ROS) scavenging. Furthermore, the transgenic lines also had enhanced salt and cold tolerance. Our findings demonstrate that AnDHN may be a good candidate gene for improving abiotic stress tolerance in crops.Key Message: Using transcriptome analysis in Ammopiptanthus nanus, we isolated a drought-responsive gene, AnDHN, that plays a key role in enhancing abiotic stress tolerance in plants, with strong functional diversification in legumes.

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“…Flavonoids are involved in the formation and development of flowers, fruits, and seeds in plants and complement antioxidant activity, UV protection, and protection against biotic and abiotic attacks by plant pathogens (Dixon and Paiva, 1995;Winkel-Shirley, 2002). Quercetin and gossypetin are known flavonoids involved in synthesizing yellow pigments in plants (Harborne, 1965;Harborne, 1968;Ververidis et al, 2007;Li et al, 2020;Yin et al, 2020;Fu et al, 2021). Aside from pigmentation, flavonoids are involved in various physiological functions under stress conditions (Nakabayashi et al, 2014;Santos-Buelga et al, 2014;Fan et al, 2016;Liang and He, 2018;Xu and Rothstein, 2018).…”

Section: Introductionmentioning

confidence: 99%

Insight into the molecular mechanisms of leaf coloration in Cymbidium ensifolium

Cao

Han

Chen³

et al. 2022

Front. Genet.

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Cymbidiumensifolium L. is a significant ornamental plant in Orchidaceae. Aside from its attractive flowers, its leaf coloration is also an important ornamental trait. However, there is an apparent lack of studies concerning the intricate mechanism of leaf coloration in C. ensifolium. In this study, we report a systematic evaluation of leaf coloration utilizing transcriptome and metabolome profiles of purple, yellow, and green leaves. In total, 40 anthocyanins and 67 flavonoids were quantified along with chlorophyll content. The tissue–transcriptome profile identified 26,499 differentially expressed genes (DEGs). The highest chlorophyll contents were identified in green leaves, followed by yellow and purple leaves. We identified key anthocyanins and flavonoids associated with leaf coloration, including cyanidin-3-O-sophoroside, naringenin-7-O-glucoside, delphinidin, cyanidin, petunidin, and quercetin, diosmetin, sinensetin, and naringenin chalcone. Moreover, genes encoding UDP-glucoronosyl, UDP-glucosyl transferase, chalcone synthesis, flavodoxin, cytochrome P450, and AMP-binding enzyme were identified as key structural genes affecting leaf coloration in C. ensifolium. In summary, copigmentation resulting from several key metabolites modulated by structural genes was identified as governing leaf coloration in C. ensifolium. Further functional verification of the identified DEGs and co-accumulation of metabolites can provide a tool to modify leaf color and improve the aesthetic value of C. ensifolium.

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Integrated metabolic profiling and transcriptome analysis of pigment accumulation in diverse petal tissues in the lily cultivar ‘Vivian’

Cited by 15 publications

References 90 publications

Regulation of MYB Transcription Factors of Anthocyanin Synthesis in Lily Flowers

Regulation of MYB Transcription Factors of Anthocyanin Synthesis in Lily Flowers

AnDHN, a Dehydrin Protein From Ammopiptanthus nanus, Mitigates the Negative Effects of Drought Stress in Plants

Insight into the molecular mechanisms of leaf coloration in Cymbidium ensifolium

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