Integrated metabolomic and transcriptomic strategies to reveal alkali-resistance mechanisms in wild soybean during post-germination growth stage

Wang, Xiaoning; Hu, Yi; Wang, Yuming; Wang, Yida; Gao, Shujuan; Zhang, Tao; Guo, Jixun; Shi, Lianxuan

doi:10.1007/s00425-023-04129-9

Cited by 2 publications

(1 citation statement)

References 56 publications

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“…These stress factors culminate in the diminished stability of plant cell membranes and cell wall structures, the inhibition of enzyme synthesis within plants, and a reduction in photosynthetic efficiency [6][7][8]. Extensive research has confirmed the impact of stress on crops such as soybeans [9], rice [10], and wheat [11] in saline-alkali soil environments. Undoubtedly, saline soils present a challenging environment for habitation.…”

Section: Introductionmentioning

confidence: 99%

Effects of NaHCO3 Stress on Black Locust (Robinia pseudoacacia L.) Physiology, Biochemistry, and Rhizosphere Bacterial Communities

Liu,

Chen,

Zhang

et al. 2023

Microorganisms

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Soil salinization has become an ecological and environmental problem that cannot be ignored. Tetraploid black locust (Robinia pseudoacacia L.) is a leguminous tree with characteristics of drought and saline-alkali tolerance. Rhizosphere bacteria are the primary functional microorganisms within the plant root system, and they play a crucial role in regulating plant growth and enhancing stress tolerance. However, there is still a lack of research on the effect of saline-alkali stress on the bacterial community structure in the rhizosphere of black locusts. In this study, we applied 0, 50, 100, and 150 mM NaHCO3 stress to diploid (2×) and tetraploid (4×) black locusts for 16 days. We used 16S rDNA sequencing to investigate the changes in the rhizosphere bacterial communities. Furthermore, we evaluated soil enzyme activity and plant physiological characteristics to explore the response of rhizosphere bacteria to NaHCO3 stress. The results demonstrated that the 4× plant exhibited superior alkali resistance compared to its 2× plant counterpart under NaHCO3 stress. Simultaneously, it was observed that low concentrations of NaHCO3 stress notably increased the abundance of rhizosphere bacteria in both plant types, while reducing their diversity. The impact of stress on the rhizosphere bacterial community weakened as the stress concentration increased. The application of NaHCO3 stress caused a significant change in the composition of the bacterial community in the rhizosphere. Additionally, alkaline salt stress influences the diversity of rhizosphere bacterial communities, which are linked to soil enzyme activities. These data will help us better understand the relationship between the dominant rhizosphere bacterial community and black locust. They will also provide a reference for further improving the alkali resistance of black locust by enhancing the soil bacterial community.

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Section: Introductionmentioning

confidence: 99%

Effects of NaHCO3 Stress on Black Locust (Robinia pseudoacacia L.) Physiology, Biochemistry, and Rhizosphere Bacterial Communities

Liu,

Chen,

Zhang

et al. 2023

Microorganisms

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Integrated Metabolomics and Transcriptomics Analyses Reveal Resistance to Salt Stress in Wild Soybean (Glycine soja) During the Post‐Germination Growth Period

Hu,

Luan,

Wang

et al. 2024

J Agronomy Crop Science

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Due to increasingly serious soil salinisation, exploring high‐quality closely related wild species is an effective means to solve food security problems. In this study, based on comprehensive metabolomics and transcriptomics analyses of the types, quantities, metabolic pathways and gene expression of small molecule metabolites in cotyledons and embryo axis/root, we report the strategies used by barren‐tolerant wild soybean (GS2) to resist salt stress during the post‐germination period. Our results showed that salt tolerance in GS2 cotyledons mainly involves the enhanced mobilisation of reserves, including lipid and sugar breakdown and utilisation, as well as protein breakdown and, in particular, the transport of amino acids to the embryo axis/root. Moreover, antioxidant capacity is enhanced through the promotion of ascorbic acid and naringin synthesis. We also found that under salt stress, the GS2 embryo axis/root accumulates proline by promoting the ornithine biosynthetic pathway, while stimulating glutathione metabolism to eliminate excess reactive oxygen species and restore oxidative balance. In addition, to establish and elongate the embryo axis/root, lignin synthesis is enhanced by the promotion of the shikimic acid pathway, which compensates for the decrease in cell wall support caused by salt stress. This study lays the foundation for developing and utilising high‐quality wild plant resources.

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Integrated metabolomic and transcriptomic strategies to reveal alkali-resistance mechanisms in wild soybean during post-germination growth stage

Cited by 2 publications

References 56 publications

Effects of NaHCO3 Stress on Black Locust (Robinia pseudoacacia L.) Physiology, Biochemistry, and Rhizosphere Bacterial Communities

Effects of NaHCO3 Stress on Black Locust (Robinia pseudoacacia L.) Physiology, Biochemistry, and Rhizosphere Bacterial Communities

Integrated Metabolomics and Transcriptomics Analyses Reveal Resistance to Salt Stress in Wild Soybean (Glycine soja) During the Post‐Germination Growth Period

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