Integrated metabolomics and mechanism to reveal the protective effect of kaempferol on pulmonary arterial hypertension

Yi, Jie; Wang, Xiaoying; Song, Kexin; Ren, Jiping; Che, Haixia; Yu, Hong; Li, Qian

doi:10.1016/j.jpba.2022.114662

Cited by 11 publications

(4 citation statements)

References 27 publications

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“…2C. Moreover, the association and change law of differential metabolites could be quickly obtained by cluster heatmap analysis [23]. The cluster heatmap showed that the SHR-NCW and SHR-Untreated groups were clearly divided into two different bands, and most differential kidney metabolites were well clustered (Fig.…”

Section: Identification and Classification Analysis Of Differential K...mentioning

confidence: 99%

Underlying anti-hypertensive mechanism of the Mizuhopecten yessoensis derived peptide NCW in spontaneously hypertensive rats via widely targeted kidney metabolomics

Xue¹,

Zhao²,

Wu³

et al. 2024

Food Science and Human Wellness

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The angiotensin-converting enzyme (ACE) inhibitory peptide NCW derived from Mizuhopecten yessoensis has been demonstrated to have significant in vivo anti-hypertensive effect, however, its anti-hypertensive mechanism is still not fully clarified. This study established a UPLC-Q-TRAP-MS/MS-based widely targeted kidney metabolomics approach to explore the changes of kidney metabolic profiles and to clarify the anti-hypertensive mechanism of peptide NCW in spontaneously hypertensive rats (SHRs). Multivariate statistical analysis indicated that the kidney metabolic profiles were clearly separated between the SHR-NCW and SHR-Untreated groups. A total of 85 metabolites were differentially regulated, and 16 metabolites were identified as the potential kidney biomarkers, e.g., 3-hydroxybutyrate, malonic acid, deoxycytidine, and L-aspartic acid. The peptide NCW might regulate kidney metabolic disorder of SHRs to alleviate hypertension by suppressing inflammation and improving nitric oxide production under the regulation of linoleic acid metabolism, folate related pathways, synthesis and degradation of ketone bodies, pyrimidine metabolism, β-alanine metabolism, and retinal metabolism.

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Section: Identification and Classification Analysis Of Differential K...mentioning

confidence: 99%

Underlying anti-hypertensive mechanism of the Mizuhopecten yessoensis derived peptide NCW in spontaneously hypertensive rats via widely targeted kidney metabolomics

Xue¹,

Zhao²,

Wu³

et al. 2024

Food Science and Human Wellness

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“…Western blot analysis 20 mg samples of lung tissue and 10 6 PASMCs were lysed (100 μL lysis buffer was added to 10 mg tissue or 10 6 cells) to extract protein samples as previously reported. 27 A BCA protein assay kit was used to determine the protein concentrations. 20 μg proteins loaded in every well of the gels were separated by SDS-PAGE and then transferred onto nitrocellulose membranes.…”

Section: Echocardiography and Hemodynamic Evaluationmentioning

confidence: 99%

Characterization of PKCα-rutin interactions and their application as a treatment strategy for pulmonary arterial hypertension by inhibiting ferroptosis

Che,

Yi,

Zhao

et al. 2024

Food Funct.

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“…Kaempferol, a natural flavonol, seems to be able to slow down the onset of PAH. Its beneficial effect on pulmonary arteries remodeling requires the upregulation of the amino acid and arachidonic acid metabolism, as revealed by metabolomics [56].…”

Section: Pah Pathogenesismentioning

confidence: 99%

Metabolomics in Pulmonary Hypertension—A Useful Tool to Provide Insights into the Dark Side of a Tricky Pathology

Bassareo,

D’Alto

2023

IJMS

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Pulmonary hypertension (PH) is a multifaceted illness causing clinical manifestations like dyspnea, fatigue, and cyanosis. If left untreated, it often evolves into irreversible pulmonary arterial hypertension (PAH), leading to death. Metabolomics is a laboratory technique capable of providing insights into the metabolic pathways that are responsible for a number of physiologic or pathologic events through the analysis of a biological fluid (such as blood, urine, and sputum) using proton nuclear magnetic resonance spectroscopy or mass spectrometry. A systematic review was finalized according to the PRISMA scheme, with the goal of providing an overview of the research papers released up to now on the application of metabolomics to PH/PAH. So, eighty-five papers were identified, of which twenty-four concerning PH, and sixty-one regarding PAH. We found that, from a metabolic standpoint, the hallmarks of the disease onset and progression are an increase in glycolysis and impaired mitochondrial respiration. Oxidation is exacerbated as well. Specific metabolic fingerprints allow the characterization of some of the specific PH and PAH subtypes. Overall, metabolomics provides insights into the biological processes happening in the body of a subject suffering from PH/PAH. The disarranged metabolic pathways underpinning the disease may be the target of new therapeutic agents. Metabolomics will allow investigators to make a step forward towards personalized medicine.

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Integrated metabolomics and mechanism to reveal the protective effect of kaempferol on pulmonary arterial hypertension

Cited by 11 publications

References 27 publications

Underlying anti-hypertensive mechanism of the Mizuhopecten yessoensis derived peptide NCW in spontaneously hypertensive rats via widely targeted kidney metabolomics

Underlying anti-hypertensive mechanism of the Mizuhopecten yessoensis derived peptide NCW in spontaneously hypertensive rats via widely targeted kidney metabolomics

Characterization of PKCα-rutin interactions and their application as a treatment strategy for pulmonary arterial hypertension by inhibiting ferroptosis

Metabolomics in Pulmonary Hypertension—A Useful Tool to Provide Insights into the Dark Side of a Tricky Pathology

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