Integrated Microfluidic Compact Disc Device with Potential Use in Both Centralized and Point-of-Care Laboratory Settings

Inganäs, M.; Dérand, Helene; Eckersten, Ann; Ekstrand, Gunnar; Honerud, Ann-Kristin; Jesson, Gérald; Thorsén, Gunnar; Söderman, Tobias; Andersson, P.

doi:10.1373/clinchem.2005.053181

Cited by 46 publications

(31 citation statements)

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“…The conventional method involves differential lysis of sperm and epithelial cells, employing centrifugation to separate the epithelial cell DNA (female fraction) from the sperm cells (male fraction). Although not routine, microfluidic control of flow on microdevices has been shown possible using centrifugation as a driving force in a compact disc‐like device (11) and for clinical applications that include preparation of plasma from whole blood (12). While the approach has not been applied to forensic differential extraction, other methods have been pursued as an alternative for obtaining male and female fractions on microfluidic devices.…”

Section: Sample Processing Stepsmentioning

confidence: 99%

Forensic DNA Analysis on Microfluidic Devices: A Review

Horsman

Bienvenue

Blasier

et al. 2007

Journal of Forensic Sciences

159

115

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The advent of microfluidic technology for genetic analysis has begun to impact forensic science. Recent advances in microfluidic separation of short-tandem-repeat (STR) fragments has provided unprecedented potential for improving speed and efficiency of DNA typing. In addition, the analytical processes associated with sample preparation--which include cell sorting, DNA extraction, DNA quantitation, and DNA amplification--can all be integrated with the STR separation in a seamless manner. The current state of these microfluidic methods as well as their advantages and potential shortcomings are detailed. Recent advances in microfluidic device technology, as they pertain to forensic DNA typing, are discussed with a focus on the forensic community.

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Section: Sample Processing Stepsmentioning

confidence: 99%

Forensic DNA Analysis on Microfluidic Devices: A Review

Horsman

Bienvenue

Blasier

et al. 2007

Journal of Forensic Sciences

159

115

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“…Numerous ''lab-on-a-disk'' products have already been launched in the market. [46][47][48][49][50][51][52][53] The paper is structured as follows. The subsequent sections describe the basic centrifugal operating principles to realize laboratory unit operations such as flow control and metering, particle (blood) sedimentation as well as mixing in batch mode and continuous flow.…”

Section: Introductionmentioning

confidence: 99%

Integrated Sample Preparation, Reaction, and Detection on a High-Frequency Centrifugal Microfluidic Platform

Steigert

Grumann

Brenner

et al. 2005

JALA: Journal of the Association for Laboratory Automation

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W e extend the toolbox of lab procedures in life sciences by development of centrifugal microfluidics for high-level process integration. This is accomplished by implementing novel functional principles for sedimentation, batch-mode mixing, frequencydependent online flow control, and optical read-out, which can be integrated into a process chain. The modular centrifugal setup comprises a microstructured disposable polymer disk as well as a reusable spinning and detection unit. We successfully developed centrifugal microfluidic technologies, which are suitable for sample preparation, process engineering, personalized diagnostics, and hematology, on this platform. (JALA 2005;10:331-41)

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“…Similarly, inherent centrifugation of samples [21][22][23] applied to sample preparation is a particular strength.The technological precursor of these systems are the centrifugal analyzers which became popular in the 1970s and 1980s [24] for automating a very limited number of simple assay steps, on rather macroscopic sample volumes, and readout on a rotor-based instrument. Currently the main areas of application are immunoassays [25][26][27][28], nucleic acid testing [29][30][31][32][33][34][35][36], and cell sorting/identification [37][38][39][40][41][42][43]. These lab-on-a-disc systems make specific use of the interplay of the frequency controlled centrifugal force and capillary action in a surface-functionalized network of micro-channels and cavities.…”

mentioning

confidence: 99%

Siphon-Induced Droplet Break-Off for Enhanced Mixing on a Centrifugal Platform

Burger¹,

Kinahan

Cayron

et al. 2019

Inventions

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We present a powerful and compact batch-mode mixing and dilution technique for centrifugal microfluidic platforms. Siphon structures are designed to discretize continuous flows into a sequence of droplets of volumes as low as 100 nL. Using a passive, self-regulating 4-step mechanism, discrete volumes of two fluids are alternatingly issued into a common intermediate chamber. At its base, a capillary valve acts as a fluidic shift register; a single droplet is held in place while two or more droplets merge and pass through the capillary stop. These merged droplets are advectively mixed as they pass through the capillary valve and into the receiving chamber. Mixing is demonstrated for various combinations of liquids such as aqueous solutions as well as saline solutions and human plasma. The mixing quality is assessed on a quantitative scale by using a colorimetric method based on the mixing of potassium thiocyanate and iron(III) chloride, and in the case of human plasma using a spectroscopic method. For instance, volumes of 5 µL have been mixed in less than 20 s. Single-step dilutions up to 1:5 of plasma in a standard phosphate buffer solution are also demonstrated. This work describes the preliminary development of the mixing method which has since been integrated into a commercially available microfluidic cartridge.Inventions 2020, 5, 1 2 of 14 microfluidic systems constitute a subset which uses the rotation of the chip, which is typically of a similar geometry as a Compact Disc™ or DVD™, to enable most pumping and valving operations. These systems can be further sub-divided into 'Bioanalytical Screening CDs' and 'Lab-on-a-Disc (LoaD)'. Bioanalytical screening CDs focus on adapting optical disc drive (ODD) technology (e.g., Compact Disc, DVD) for biological detection [9]. This technology takes advantage of the high-density data storage capability as well as the favorable pricing for its main constituents, i.e., the optical pickup unit, the spindle drive, and the optical disc substrates [10][11][12][13][14].The LoaD [15][16][17][18][19][20] is typically used to automate the standard laboratory steps conducted in a biology lab to enable a fully automated sample-to-answer system. The LoaD is particularly applicable for deployment in the field (point-of-use) or at the hospital bed-side (point-of-care) as the chips can be loaded at atmospheric pressure (via pipette or syrette) without issues regarding sealing or priming. The use of centrifugation for pumping also reduces the cost and complexity of support instrumentation as just low-cost spindle motors are required rather than specialized micropumps. Similarly, inherent centrifugation of samples [21][22][23] applied to sample preparation is a particular strength.The technological precursor of these systems are the centrifugal analyzers which became popular in the 1970s and 1980s [24] for automating a very limited number of simple assay steps, on rather macroscopic sample volumes, and readout on a rotor-based instrument. Currently the main areas of application are immuno...

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Integrated Microfluidic Compact Disc Device with Potential Use in Both Centralized and Point-of-Care Laboratory Settings

Cited by 46 publications

References 0 publications

Forensic DNA Analysis on Microfluidic Devices: A Review

Forensic DNA Analysis on Microfluidic Devices: A Review

Integrated Sample Preparation, Reaction, and Detection on a High-Frequency Centrifugal Microfluidic Platform

Siphon-Induced Droplet Break-Off for Enhanced Mixing on a Centrifugal Platform

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