Integrated microfluidic device to monitor unseen Escherichia coli contamination in mammalian cell culture

Dervisevic, Esma; Dervisevic, Muamer; Ang, Bryan; Carthew, James; Tuck, Kellie L.; Voelcker, Nicolas H.; Cadarso, Víctor J.

doi:10.1016/j.snb.2022.131522

Cited by 4 publications

(2 citation statements)

References 33 publications

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“…PAT has been extensively used for contamination detection in bioprocesses, some exploring combinations of two or more methods, and some modifying and enhancing the current methods [3,59,60]. For instance, a light microscope and/or molecular probes are traditionally used to detect microbial contamination in cell culture media.…”

Section: Process Analytical Technology (Pat)mentioning

confidence: 99%

Methods and Analysis of Biological Contaminants in the Biomanufacturing Industry

et al. 2023

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The advent of bioprocessing has revolutionized the biomanufacturing industry, leading to the rise of biotherapeutics derived from biologic products such as chimeric antigen receptor (CAR) T-cells used for targeted cancer treatment and the Vero cell line for the production of viral vectors and vaccines. Despite these promising developments, most biologic products are characterized by fragile macromolecular structures that are heterogenous with a purity profile that varies with each batch making them susceptible to microorganism contamination. Regulatory oversight of biologic products is imperative to ensure adherence to good manufacturing practices and compliance with quality management systems. Current quality assurance protocols during production include monoclonality during cell line development, real-time monitoring of process parameters, flow cytometry for microbial monitoring, polymerase chain reaction, and immunoassay techniques to amplify DNA sequences related to bacterial or biological contaminants. FDA guidance recommends the implementation of process analytical technology within biomanufacturing production to measure critical quality parameters, which includes screening for potential biological contamination. Future advancements in bioprocess monitoring and control should capitalize on providing cheap, real-time, and sensitive detection. Biosensors, mass spectrometry, and polymerase chain reaction present robust, rapid, and real-time capabilities for multiplexed detection of contaminant analytes and have shown promise in meeting these needs. This review discusses the main biological contaminants of bioprocesses, European Union and FDA regulatory guidelines for monitoring and control within biologics production, existing methods and their limitations, and future advancements for biological contamination detection.

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Section: Process Analytical Technology (Pat)mentioning

confidence: 99%

Methods and Analysis of Biological Contaminants in the Biomanufacturing Industry

et al. 2023

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“…Hence, this development step represents one of the greatest challenges in this field. In this context, microfluidics has acquired huge importance over the last years, as a branch of science and technology that allows accurate manipulation and monitoring of the fluids on micrometric scale channels. − Microfluidic devices have applications in several areas, including chemical synthesis, molecular biology, tissue engineering, and NP screening in terms of transport and efficiency . Due to countless advances and innovations, microfluidic devices are expected to be the key to improving the controlled synthesis of NPs and accelerating their transition to clinical evaluation.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic Devices: A Tool for Nanoparticle Synthesis and Performance Evaluation

Gimondi,

Ferreira,

Reis

et al. 2023

ACS Nano

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The use of nanoparticles (NPs) in nanomedicine holds great promise for the treatment of diseases for which conventional therapies present serious limitations. Additionally, NPs can drastically improve early diagnosis and follow-up of many disorders. However, to harness their full capabilities, they must be precisely designed, produced, and tested in relevant models. Microfluidic systems can simulate dynamic fluid flows, gradients, specific microenvironments, and multiorgan complexes, providing an efficient and cost-effective approach for both NPs synthesis and screening. Microfluidic technologies allow for the synthesis of NPs under controlled conditions, enhancing batch-to-batch reproducibility. Moreover, due to the versatility of microfluidic devices, it is possible to generate and customize endless platforms for rapid and efficient in vitro and in vivo screening of NPs’ performance. Indeed, microfluidic devices show great potential as advanced systems for small organism manipulation and immobilization. In this review, first we summarize the major microfluidic platforms that allow for controlled NPs synthesis. Next, we will discuss the most innovative microfluidic platforms that enable mimicking in vitro environments as well as give insights into organism-on-a-chip and their promising application for NPs screening. We conclude this review with a critical assessment of the current challenges and possible future directions of microfluidic systems in NPs synthesis and screening to impact the field of nanomedicine.

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Bioreactor contamination monitoring using off-gassed volatile organic compounds (VOCs)

Linderholm,

Bhandari,

Borras

et al. 2024

Anal Bioanal Chem

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Integrated microfluidic device to monitor unseen Escherichia coli contamination in mammalian cell culture

Cited by 4 publications

References 33 publications

Methods and Analysis of Biological Contaminants in the Biomanufacturing Industry

Methods and Analysis of Biological Contaminants in the Biomanufacturing Industry

Microfluidic Devices: A Tool for Nanoparticle Synthesis and Performance Evaluation

Bioreactor contamination monitoring using off-gassed volatile organic compounds (VOCs)

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