Integrated Microfluidic Preconcentration and Nucleic Amplification System for Detection of Influenza A Virus H1N1 in Saliva

Kim, Yong Hee; Abafogi, Abdurhaman Teyib; Tran, Buu Minh; Kim, Jae-Won; Lee, Ji Oon; Chen, Zhenzhong; Bae, Pan Kee; Park, Kyoungsook; Shin, Yong‐Beom; Noort, Danny van; Lee, Nae Yoon; Park, Sungsu

doi:10.3390/mi11020203

Cited by 27 publications

(22 citation statements)

References 38 publications

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“…For this reason, it is better to pre-concentrate the virus from a large volume sample before nucleic acid extraction. For instance, Kim et al (2020a) designed a microfluidic pre-concentration and nucleic amplification system (FPNAS) in which the anti-H1N1, antibody-conjugated, magnetic nanoparticles pre-concentrated the H1N1 virus from a large volume of saliva and, then, RT-PCR was used to detect the H1N1 virus. This approach improved the LOD by 10 orders of magnitude compared to non-treated samples ( Fig.…”

Section: Potential Biosensors For Point-of-care Sars-cov-2 Detectionmentioning

confidence: 99%

“…6 (A) Schematic describing the operation protocol of on-chip pre-concentration and nucleic acid amplification. Reproduced with permission from ( Kim et al, 2020a ). (B) Workflow of free-flow electrophoretic virus particles at pre-concentration, followed by thermal lysis and gel-electrophoretic nucleic acid extraction.…”

Section: Potential Biosensors For Point-of-care Sars-cov-2 Detectionmentioning

confidence: 99%

See 1 more Smart Citation

Detection of COVID-19: A review of the current literature and future perspectives

Liu

Wang

et al. 2020

Biosensors and Bioelectronics

353

309

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The rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to the coronavirus disease 2019 (COVID-19) worldwide pandemic. This unprecedented situation has garnered worldwide attention. An effective strategy for controlling the COVID-19 pandemic is to develop highly accurate methods for the rapid identification and isolation of SARS-CoV-2 infected patients. Many companies and institutes are therefore striving to develop effective methods for the rapid detection of SARS-CoV-2 ribonucleic acid (RNA), antibodies, antigens, and the virus. In this review, we summarize the structure of the SARS-CoV-2 virus, its genome and gene expression characteristics, and the current progression of SARS-CoV-2 RNA, antibodies, antigens, and virus detection. Further, we discuss the reasons for the observed false-negative and false-positive RNA and antibody detection results in practical clinical applications. Finally, we provide a review of the biosensors which hold promising potential for point-of-care detection of COVID-19 patients. This review thereby provides general guidelines for both scientists in the biosensing research community and for those in the biosensor industry to develop a highly sensitive and accurate point-of-care COVID-19 detection system, which would be of enormous benefit for controlling the current COVID-19 pandemic.

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Section: Potential Biosensors For Point-of-care Sars-cov-2 Detectionmentioning

confidence: 99%

Section: Potential Biosensors For Point-of-care Sars-cov-2 Detectionmentioning

confidence: 99%

Detection of COVID-19: A review of the current literature and future perspectives

Liu

Wang

et al. 2020

Biosensors and Bioelectronics

353

309

View full text Add to dashboard Cite

show abstract

“…Various exciting directions are expected for the future of LOC highly integrated devices for diagnostic and therapeutic are some of the interesting applications of LOC devices. For instance, rapid detection of viral infections [152,153] and early detection of cancer biomarkers [154,155] directly from body fluids are some of the highly attractive subjects in this area.…”

Section: Discussion and Future Perspectivesmentioning

confidence: 99%

Evolution of Biochip Technology: A Review from Lab-on-a-Chip to Organ-on-a-Chip

et al. 2020

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Following the advancements in microfluidics and lab-on-a-chip (LOC) technologies, a novel biomedical application for microfluidic based devices has emerged in recent years and microengineered cell culture platforms have been created. These micro-devices, known as organ-on-a-chip (OOC) platforms mimic the in vivo like microenvironment of living organs and offer more physiologically relevant in vitro models of human organs. Consequently, the concept of OOC has gained great attention from researchers in the field worldwide to offer powerful tools for biomedical researches including disease modeling, drug development, etc. This review highlights the background of biochip development. Herein, we focus on applications of LOC devices as a versatile tool for POC applications. We also review current progress in OOC platforms towards body-on-a-chip, and we provide concluding remarks and future perspectives for OOC platforms for POC applications.

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“…Kim et al recently designed a microfluidic‐based method for detecting H1N1 influenza, and the results suggested that the limit of detection (LOD) of molecular diagnostics for the virus can be lowered by systematically combining immunomagnetic separation and RT‐PCR in one microfluidic device 40 . Moreover, RT‐PCR in situ has been successfully used for the diagnosis of Zika virus 68 .…”

Section: Microfluidic Devicesmentioning

confidence: 99%

“…As a result, these devices may also help detect SARS‐CoV‐2 and accelerate the process of diagnosis and rehabilitation, ultimately lowering the death rate. These devices can use several techniques such as RT‐PCR, RT‐LAMP, nested PCR, nucleic acid hybridization, ELISA, or fluorescence‐based Assays 40‐44 …”

Section: Introductionmentioning

confidence: 99%

Microfluidic devices for detection of RNA viruses

Basiri

Heidari

Nadi

et al. 2020

Reviews in Medical Virology

105

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Summary There is a long way to go before the coronavirus disease 2019 (Covid‐19) outbreak comes under control. qRT‐PCR is currently used for the detection of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), the causative agent of Covid‐19, but it is expensive, time‐consuming, and not as sensitive as it should be. Finding a rapid, easy‐to‐use, and cheap diagnostic method is necessary to help control the current outbreak. Microfluidic systems provide a platform for many diagnostic tests, including RT‐PCR, RT‐LAMP, nested‐PCR, nucleic acid hybridization, ELISA, fluorescence‐Based Assays, rolling circle amplification, aptamers, sample preparation multiplexer (SPM), Porous Silicon Nanowire Forest, silica sol‐gel coating/bonding, and CRISPR. They promise faster, cheaper, and easy‐to‐use methods with higher sensitivity, so microfluidic devices have a high potential to be an alternative method for the detection of viral RNA. These devices have previously been used to detect RNA viruses such as H1N1, Zika, HAV, HIV, and norovirus, with acceptable results. This paper provides an overview of microfluidic systems as diagnostic methods for RNA viruses with a focus on SARS‐CoV‐2.

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Integrated Microfluidic Preconcentration and Nucleic Amplification System for Detection of Influenza A Virus H1N1 in Saliva

Cited by 27 publications

References 38 publications

Detection of COVID-19: A review of the current literature and future perspectives

Detection of COVID-19: A review of the current literature and future perspectives

Evolution of Biochip Technology: A Review from Lab-on-a-Chip to Organ-on-a-Chip

Microfluidic devices for detection of RNA viruses

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