Integrated microfluidic systems with sample preparation and nucleic acid amplification

Yin, Juxin; Suo, Yuanjie; Zou, Zheyu; Sun, Jingjing; Zhang, Shan; Wang, Ben; Xu, Yawei; Darland, Diane C.; Zhao, Julia Xiaojun; Mu, Ying

doi:10.1039/c9lc00389d

Cited by 98 publications

(54 citation statements)

References 158 publications

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“…The microfluidic system was developed over 20 years ago to translate conventional bulk models into miniaturized systems in diverse areas, including chemical and biological experiments. 11,12 The rapid acceptance of miniaturized microfluidic systems has largely been driven by the advances in various cell-based assays, including direct and indirect interactions, the release and reception of soluble factors, and the transmission of electrical signals. 13 Microfluidic systems provide significant advantages for these research fields, such as the precise control of microscale structures and fluid flow, as well as spatiotemporal control of cells, chemical concentration, and temperature or oxygen gradients.…”

Section: Methods For Chemical Gradient Generationmentioning

confidence: 99%

Recent Developments in Bacterial Chemotaxis Analysis Based on the Microfluidic System

Jeong

2021

SLAS Technology

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Section: Methods For Chemical Gradient Generationmentioning

confidence: 99%

Recent Developments in Bacterial Chemotaxis Analysis Based on the Microfluidic System

Jeong

2021

SLAS Technology

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“…Microfluidics featuring at reduced sample consumption and shortened reaction time has been extensively studied for nucleic acid analysis, because of its capability to integrate different operation units, such as sample pretreatment, nucleic acid extraction and amplification, and signal detection into a single microfluidic device [349][350][351][352]. A microfluidic cassette was designed for nucleic acid detection of Zika Virus [353].…”

Section: Microfluidic Devicementioning

confidence: 99%

Nucleic Acids Analysis

et al. 2020

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In recent years, multi-modal entity linking (MEL) has garnered increasing attention in the research community due to its significance in numerous multi-modal applications. Video, as a popular means of information transmission, has become prevalent in people's daily lives. However, most existing MEL methods primarily focus on linking textual and visual mentions or offline videos's mentions to entities in multi-modal knowledge bases, with limited efforts devoted to linking mentions within online video content. In this paper, we propose a task called Online Video Entity Linking (OVEL), aiming to establish connections between mentions in online videos and a knowledge base with high accuracy and timeliness. To facilitate the research works of OVEL, we specifically concentrate on live delivery scenarios and construct a live delivery entity linking dataset called LIVE. Besides, we propose an evaluation metric that considers timelessness, robustness, and accuracy. Furthermore, to effectively handle OVEL task, we leverage a memory block managed by a Large Language Model and retrieve entity candidates from the knowledge base to augment LLM performance on memory management. The experimental results prove the effectiveness and efficiency of our method.

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“…After the first demonstration of a miniaturized total chemical analysis system (μ-TAS) by Manz et al (1990) , researchers in the last 30 years have developed numerous molecular detection systems utilizing microfluidic ( Kolluri et al, 2017 ; Koo et al, 2017 ; Liu et al, 2011 ; Q. Liu et al, 2018a , Liu et al, 2018c ; Petralia et al, 2013 ; Wu et al, 2014 ; Yan et al, 2017 ; Yin et al, 2019b ; Zhang et al, 2016 ) or paper-based devices ( Choi et al, 2016 ; Deng et al, 2017 ; Liu et al, 2017 ; Loo et al, 2017 ; Rodriguez et al, 2015 ). Furthermore, several commercial platforms such as GeneXpert Systems (Cepheid, USA), ARIES Systems (Luminex, USA), BioFire FilmArray Torch (BioFire Diagnostics, USA), and Integrated Cycler (Focus Diagnostics, USA) have been developed for rapid molecular diagnosis of human diseases.…”

Section: Introductionmentioning

confidence: 99%

Advances in point-of-care nucleic acid extraction technologies for rapid diagnosis of human and plant diseases

Paul

Ostermann

Wei

2020

Biosensors and Bioelectronics

135

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Global health and food security constantly face the challenge of emerging human and plant diseases caused by bacteria, viruses, fungi, and other pathogens. Disease outbreaks such as SARS, MERS, Swine Flu, Ebola, and COVID-19 (on-going) have caused suffering, death, and economic losses worldwide. To prevent the spread of disease and protect human populations, rapid point-of-care (POC) molecular diagnosis of human and plant diseases play an increasingly crucial role. Nucleic acid-based molecular diagnosis reveals valuable information at the genomic level about the identity of the disease-causing pathogens and their pathogenesis, which help researchers, healthcare professionals, and patients to detect the presence of pathogens, track the spread of disease, and guide treatment more efficiently. A typical nucleic acid-based diagnostic test consists of three major steps: nucleic acid extraction, amplification, and amplicon detection. Among these steps, nucleic acid extraction is the first step of sample preparation, which remains one of the main challenges when converting laboratory molecular assays into POC tests. Sample preparation from human and plant specimens is a time-consuming and multi-step process, which requires well-equipped laboratories and skilled lab personnel. To perform rapid molecular diagnosis in resource-limited settings, simpler and instrument-free nucleic acid extraction techniques are required to improve the speed of field detection with minimal human intervention. This review summarizes the recent advances in POC nucleic acid extraction technologies. In particular, this review focuses on novel devices or methods that have demonstrated applicability and robustness for the isolation of high-quality nucleic acid from complex raw samples, such as human blood, saliva, sputum, nasal swabs, urine, and plant tissues. The integration of these rapid nucleic acid preparation methods with miniaturized assay and sensor technologies would pave the road for the “sample-in-result-out” diagnosis of human and plant diseases, especially in remote or resource-limited settings.

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Integrated microfluidic systems with sample preparation and nucleic acid amplification

Abstract: Integrated microfluidic systems with sample preparation and nucleic acid amplification can increase treatment efficiency and improve care.

Cited by 98 publications

References 158 publications

Recent Developments in Bacterial Chemotaxis Analysis Based on the Microfluidic System

Recent Developments in Bacterial Chemotaxis Analysis Based on the Microfluidic System

Nucleic Acids Analysis

Advances in point-of-care nucleic acid extraction technologies for rapid diagnosis of human and plant diseases

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