Integrated MicroRNA and Secretome Analysis of Human Endometrial Organoids Reveal the miR-3194-5p/Aquaporin/S100A9 Module in Regulating Trophoblast Functions

Dong, Yang; Li, Jianlin; Cao, Dandan; Zhong, Jiangming; Liu, Xiaofeng; Duan, Yong‐Gang; Lee, Ckf; Yeung, WilliamS.B.; Lee, Cheuk‐Lun; Chiu, Philip C.N.

doi:10.1016/j.mcpro.2023.100526

Cited by 5 publications

(1 citation statement)

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“…These organoids show potent stability in retaining genetic traits during cell division and differentiation, and their ability to restore secretory characteristics under thawing conditions after cryopreservation has been fully tested [ 30 ]. Additionally, positive responses to estrogen and progesterone have been observed in epithelial cells of endometrial organoids, paving the way for studies on endometrial-related diseases [ 31 , 32 ]. Notably, endometrial stromal, angiogenic, and immune cells that have been identified in the human endometrium can be cultured along with epithelial SCs in vitro.…”

Section: Engineering Technologies Focusing On the Endometriummentioning

confidence: 99%

Bioengineering approaches for the endometrial research and application

Dai,

Liang,

Guo

et al. 2024

Materials Today Bio

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Section: Engineering Technologies Focusing On the Endometriummentioning

confidence: 99%

Bioengineering approaches for the endometrial research and application

Dai,

Liang,

Guo

et al. 2024

Materials Today Bio

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JPT2 Affects Trophoblast Functions and Macrophage Polarization and Metabolism, and Acts as a Potential Therapeutic Target for Recurrent Spontaneous Abortion

Chen,

Song,

et al. 2024

Advanced Science

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Recurrent spontaneous abortion (RSA) is a pregnancy‐related condition with complex etiology. Trophoblast dysfunction and abnormal macrophage polarization and metabolism are associated with RSA; however, the underlying mechanisms remain unknown. Jupiter microtubule‐associated homolog 2 (JPT2) is essential for calcium mobilization; however, its role in RSA remains unclear. In this study, it is found that the expression levels of JPT2, a nicotinic acid adenine dinucleotide phosphate‐binding protein, are decreased in the villous tissues of patients with RSA and placental tissues of miscarried mice. Mechanistically, it is unexpectedly found that abnormal JPT2 expression regulates trophoblast function and thus involvement in RSA via c‐Jun N‐terminal kinase (JNK) signaling, but not via calcium mobilization. Specifically, on the one hand, JPT2 deficiency inhibits trophoblast adhesion, migration, and invasion by inhibiting the JNK/atypical chemokine receptor 3 axis. On the other hand, trophoblast JPT2 deficiency contributes to M1 macrophage polarization by promoting the accumulation of citrate and reactive oxygen species via inhibition of the JNK/interleukin‐6 axis. Self‐complementary adeno‐associated virus 9‐JPT2 treatment alleviates embryonic resorption in abortion‐prone mice. In summary, this study reveals that JPT2 mediates the remodeling of the immune microenvironment at the maternal–fetal interface, suggesting its potential as a therapeutic target for RSA.

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