Integrated multi-omics analyses reveal the key microbial phylotypes affecting anaerobic digestion performance under ammonia stress

Zhang, Hong; Yuan, Wenduo; Dong, Qin; Wu, Di; Yang, Pingjin; Peng, Yun; Li, Lei; Peng, Xiangjun

doi:10.1016/j.watres.2022.118152

Cited by 71 publications

(9 citation statements)

References 18 publications

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“…When compared with the results of previous studies on AD of water hyacinth with dung inoculum, this study recorded a lower methane production (Tasnim et al 2017 ). Reports by Westerholm et al ( 2018 ) and Zhang et al ( 2022 ) similarly confirm the detrimental impact of ammonia on synthrophic acetate oxidizing bacteria during AD. Additionally, exclusion of foreign microbes such as those found in cow dung inoculum in the digesters could have also contributed to reduced methane production, due to inefficiency of the indigenous microbial community of water hyacinth inoculum to improve methane production (Horváth et al 2016 ).…”

Section: Discussionmentioning

confidence: 61%

Bioaugmentation potential of inoculum derived from anaerobic digestion feedstock for enhanced methane production using water hyacinth

Linda

Roopnarain

Tekere

et al. 2023

World J Microbiol Biotechnol

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The utilisation of water hyacinth for production of biogas is considered to be a solution to both its control and the global renewable energy challenge. In this instance, an investigation was conducted to evaluate the potential of water hyacinth inoculum to enhance methane production during anaerobic digestion (AD). Chopped whole water hyacinth (10% (w/v)) was digested to prepare an inoculum consisting mainly of water hyacinth indigenous microbes. The inoculum was incorporated in the AD of freshly chopped whole water hyacinth to set up different ratios of water hyacinth inoculum and water hyacinth mixture with appropriate controls. The results of batch tests with water hyacinth inoculum showed a maximal cumulative volume of 211.67 ml of methane after 29 days of AD as opposed to 88.6 ml of methane generated from the control treatment without inoculum. In addition to improving methane production, inclusion of water hyacinth inoculum reduced the electrical conductivity (EC) values of the resultant digestate, and, amplification of nifH and phoD genes in the digestate accentuates it as a potential soil ameliorant. This study provides an insight into the potential of water hyacinth inoculum to enhance methane production and contribute to the feasibility of the digestate as a soil fertility enhancer.

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Section: Discussionmentioning

confidence: 61%

Bioaugmentation potential of inoculum derived from anaerobic digestion feedstock for enhanced methane production using water hyacinth

Linda

Roopnarain

Tekere

et al. 2023

World J Microbiol Biotechnol

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“…While previous studies have examined the adaptation of the microbiome to increasing levels of ammonia, the mRNA-level response, particularly regarding mechanisms involved in the recovery from ammonia-induced stress, remains poorly understood. More into detail, integrated metagenomics and metaproteomics focused only on elucidating the impact of ammonia on methanogens and syntrophs, as well as activity of key enzymes during AD. , However, upregulation of enzymes under ammonia-stressed conditions, potentially leading to a mechanism for controlling ammonia toxicity and stabilizing the AD process, is still largely unexplored. Therefore, it is important to use genome-centric metatranscriptomics to map the early response of the microbiota, which can aid in understanding and managing the turn-on and turn-off steps of the AD process.…”

Section: Resultsmentioning

confidence: 99%

Decoding Microbial Responses to Ammonia Shock Loads in Biogas Reactors through Metagenomics and Metatranscriptomics

Gaspari,

Ghiotto,

Centurion

et al. 2023

Environ. Sci. Technol.

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The presence of elevated ammonia levels is widely recognized as a significant contributor to process inhibition in biogas production, posing a common challenge for biogas plant operators. The present study employed a combination of biochemical, genome-centric metagenomic and metatranscriptomic data to investigate the response of the biogas microbiome to two shock loads induced by single pulses of elevated ammonia concentrations (i.e., 1.5 g NH 4 + /L R and 5 g NH 4 + /L R ). The analysis revealed a microbial community of high complexity consisting of 364 Metagenome Assembled Genomes (MAGs). The hydrogenotrophic pathway was the primary route for methane production during the entire experiment, confirming its efficiency even at high ammonia concentrations. Additionally, metatranscriptomic analysis uncovered a metabolic shift in the methanogens Methanothrix sp. MA6 and Methanosarcina f lavescens MX5, which switched their metabolism from the acetoclastic to the CO 2 reduction route during the second shock. Furthermore, multiple genes associated with mechanisms for maintaining osmotic balance in the cell were upregulated, emphasizing the critical role of osmoprotection in the rapid response to the presence of ammonia. Finally, this study offers insights into the transcriptional response of an anaerobic digestion community, specifically focusing on the mechanisms involved in recovering from ammonia-induced stress.

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“…This was approximately 3.5 times less than that of the Control digester, at 714 mg-FAN L −1 . A study by Zhang, Yuan [ 37 ] found that 337 mg-FAN L −1 decreased methane yield by 65 %. This aligns with the findings of this study where a significant reduction in methane yield was experienced in Control digesters ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Wood biochar enhances methanogenesis in the anaerobic digestion of chicken manure under ammonia inhibition conditions

Ngo,

Khudur,

Krohn

et al. 2023

Heliyon

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Integrated multi-omics analyses reveal the key microbial phylotypes affecting anaerobic digestion performance under ammonia stress

Cited by 71 publications

References 18 publications

Bioaugmentation potential of inoculum derived from anaerobic digestion feedstock for enhanced methane production using water hyacinth

Bioaugmentation potential of inoculum derived from anaerobic digestion feedstock for enhanced methane production using water hyacinth

Decoding Microbial Responses to Ammonia Shock Loads in Biogas Reactors through Metagenomics and Metatranscriptomics

Wood biochar enhances methanogenesis in the anaerobic digestion of chicken manure under ammonia inhibition conditions

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