Integrated multi-omics reveals cellular and molecular interactions governing the invasive niche of basal cell carcinoma

Yerly, Laura; Pich-Bavastro, Christine; Domizio, Jérémy Di; Wyss, Tania; Tissot-Renaud, Stéphanie; Cangkrama, Michael; Gilliet, Michel; Werner, Sabine; Kuonen, François

doi:10.1038/s41467-022-32670-w

Cited by 33 publications

(31 citation statements)

References 71 publications

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“…The mechanisms by which aggressive BCCs develop are beginning to be fully elucidated. It was recently reported that transcriptional reprogramming occurs at the leading edge of the tumor–stroma interface in infiltrative BCC cases, thereby giving rise to tumor cells with a collective migratory phenotype and cancer-associated fibroblasts with extracellular matrix remodeling properties [ 25 ]. Moreover, the activin A signaling pathway regulates transcriptional tumor–stroma interactions and spatial organization.…”

Section: Discussionmentioning

confidence: 99%

Recurrence or de novo? Intradermal Basal Cell Carcinoma of the Scrotum: A Report of Two Cases

et al. 2023

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Basal cell carcinoma (BCC) is the most common cutaneous malignancy, usually occurring in sun-exposed areas. Although BCC in the scrotal region is uncommon, it carries a higher risk of metastasis than BCC at other sites. Here, we report two cases of BCC that developed in the scrotal region: Case 1 presented as a superficial nodule and Case 2 as a subcutaneous nodule. Histopathologically, both tumors lacked continuity with the surface epidermis and formed an intradermal nodule. In Case 1, BCC occurred for the first time and presumably developed de novo. Case 2 underwent excision of a scrotal BCC 5 years previously, and the histopathological diagnosis at that time was nodular BCC. However, when the original specimen was re-examined, it was determined that, although the tumor had been completely resected, part of the lesion had moved away from the nodular area to represent a micronodular phenotype, an aggressive BCC subtype. We hypothesized that partial evolution from a nodular to a micronodular phenotype may have contributed to the recurrence of BCC in Case 2.

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Section: Discussionmentioning

confidence: 99%

Recurrence or de novo? Intradermal Basal Cell Carcinoma of the Scrotum: A Report of Two Cases

et al. 2023

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“…35 In basal cell carcinoma (BCC), the tumour-stroma interface was genetically separated, with a total of 86 genes found to be differentially expressed between the infiltrative and nodular tumour regions. 34 A high percentage of the genes was observed in a fibroblast cluster which was reported to drive BCC infiltration. 34 The spatial genomic landscape of tissues in clinical trials and drug development and testing can be particularly useful in identifying specific areas responsive to the drug and deciphering pathways that are associated with each response.…”

Section: Skin Cancermentioning

confidence: 99%

“…34 A high percentage of the genes was observed in a fibroblast cluster which was reported to drive BCC infiltration. 34 The spatial genomic landscape of tissues in clinical trials and drug development and testing can be particularly useful in identifying specific areas responsive to the drug and deciphering pathways that are associated with each response. Head and neck squamous cell carcinomas (HNSCC) are classified as the most common malignancies in the pharynx, larynx and oral cavity.…”

Section: Skin Cancermentioning

confidence: 99%

“…Ligand receptor mapping revealed that TSK cells participated in autocrine and paracrine interactions mostly with cancer‐associated fibroblasts (CAFs), endothelial cells and macrophages that may contribute to tumour progression, immunosuppression and heterogeneity 35 . In basal cell carcinoma (BCC), the tumour‐stroma interface was genetically separated, with a total of 86 genes found to be differentially expressed between the infiltrative and nodular tumour regions 34 . A high percentage of the genes was observed in a fibroblast cluster which was reported to drive BCC infiltration 34 …”

Section: Applications In Skin Researchmentioning

confidence: 99%

“…In basal cell carcinoma (BCC), the tumour‐stroma interface was genetically separated, with a total of 86 genes found to be differentially expressed between the infiltrative and nodular tumour regions 34 . A high percentage of the genes was observed in a fibroblast cluster which was reported to drive BCC infiltration 34 …”

Section: Applications In Skin Researchmentioning

confidence: 99%

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Spatial transcriptomics in human skin research

Tekkela

Theocharidis

McGrath

et al. 2023

Experimental Dermatology

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Tissues and organs of multicellular organisms are spatially organised by the compartmentalisation of cell types and subpopulations that are coordinated in internal gene regulatory networks, and by signalling from their surrounding tissue environment. In order to perform a subset of biologic processes, these cells differ in terms of localisation, morphology and gene expression patterns. 1 The development of single-cell methods has enabled studies that can retain cellular properties and heterogeneity at a single-cell resolution, which has led to a more detailed understanding of physiologic and disease conditions. 2 One of the most popular methods, single-cell RNA-sequencing (scRNA-seq), has made it possible to profile the whole transcriptome of each individual cell. 3,4 However, it results in the loss of information on spatial relationships among the cell populations since it entails dismantling of the original tissue into single-cell suspensions through mechanical and enzymatic dissociation steps, which could perturb or alter the cell's gene expression. 5 Recently, various techniques have been developed aiming for transcriptome mapping while preserving the spatial location of the expressed transcripts within intact tissues. 6 These methods, collectively known as spatial transcriptomics (ST), have been voted as the method of the year 2020 by Nature Methods and expect to provide remarkable novel insights in the

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Spatially Resolved Transcriptomics Technology Facilitates Cancer Research

Wang,

Zhi,

et al. 2023

Advanced Science

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Single cell RNA sequencing (scRNA‐seq) provides a great convenience for studying tumor occurrence and development for its ability to study gene expression at the individual cell level. However, patient‐derived tumor tissues are composed of multiple types of cells including tumor cells and adjacent non‐malignant cells such as stromal cells and immune cells. The spatial locations of various cells in situ tissues plays a pivotal role in the occurrence and development of tumors, which cannot be elucidated by scRNA‐seq alone. Spatially resolved transcriptomics (SRT) technology emerges timely to explore the unrecognized relationship between the spatial background of a particular cell and its functions, and is increasingly used in cancer research. This review provides a systematic overview of the SRT technologies that are developed, in particular the more widely used cutting‐edge SRT technologies based on next‐generation sequencing (NGS). In addition, the main achievements by SRT technologies in precisely unveiling the underappreciated spatial locations on gene expression and cell function with unprecedented high‐resolution in cancer research are emphasized, with the aim of developing more effective clinical therapeutics oriented to a deeper understanding of the interaction between tumor cells and surrounding non‐malignant cells.

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Integrated multi-omics reveals cellular and molecular interactions governing the invasive niche of basal cell carcinoma

Cited by 33 publications

References 71 publications

Recurrence or de novo? Intradermal Basal Cell Carcinoma of the Scrotum: A Report of Two Cases

Recurrence or de novo? Intradermal Basal Cell Carcinoma of the Scrotum: A Report of Two Cases

Spatial transcriptomics in human skin research

Spatially Resolved Transcriptomics Technology Facilitates Cancer Research

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