Integrated Multilevel Surveillance of the World's Infecting Microbes and Their Resistance to Antimicrobial Agents

O’Brien, Thomas F.; Stelling, John

doi:10.1128/cmr.00021-10

Cited by 78 publications

(73 citation statements)

References 125 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Antimicrobial susceptibility data analyses were made using WHONET software (22). Univariate analysis was done using the chi-square test or Fisher's exact test (if the number was less than 10).…”

Section: Methodsmentioning

confidence: 99%

Epidemiology and Molecular Characterization of Macrolide-Resistant Streptococcus pyogenes in Taiwan

et al. 2014

View full text Add to dashboard Cite

Recovery from respiratory (throat swab) specimens was an independent factor associated with ERY resistance. emm1 and emm11 GAS isolates were significantly associated with ERY s , while emm22 was detected only in ERY r GAS. In addition, emm106 isolates were prevalent among the abscess/pus isolates, whereas emm12 isolates were strongly associated with a respiratory (throat) origin. In addition to identifying factors associated with ERY resistance in GAS, our study provides helpful information on the changing GAS epidemiology in Taiwan.

show abstract

Section: Methodsmentioning

confidence: 99%

Epidemiology and Molecular Characterization of Macrolide-Resistant Streptococcus pyogenes in Taiwan

et al. 2014

View full text Add to dashboard Cite

show abstract

“…One of the advantages of well-organized surveillance is larger number of collected samples, which doubtlessly highlight which pathogen, when isolated, is the biggest threat [30]. Significant increase in collected blood-culture bottles and detection of MRSA after initiation of national surveillance in our study are direct effects of more systematic data collection and reporting in laboratories during the active surveillance.…”

Section: Discussionmentioning

confidence: 63%

Differences in MRSA prevalence and resistance patterns in a tertiary center before and after joining an international program for surveillance of antimicrobial resistance

Djurić

Jovanović²,

Branka³

et al. 2017

Acta Microbiologica et Immunologica Hungarica

View full text Add to dashboard Cite

Methicillin-resistant Staphylococcus aureus (MRSA) emerged as one of the most important causes of hospital-acquired bloodstream infections (BSIs), especially the multidrug resistant clones. The aim of the present study was to compare prevalence and resistance patterns of MRSA bacteremia in the major tertiary-care academic and referral center in Serbia before and after implementing an active antimicrobial resistance (AMR) surveillance. Laboratory-based before-after study was conducted during a two-year period (January 2012 to December 2013) in Clinical Centre of Serbia. Isolation and identification of bacterial strains were done following standard microbiological procedures. During the AMR surveillance, nearly twice more bloodstream samples were collected compared to the year without surveillance (1,528 vs. 855). In total, 43 isolates of MRSA were identified. MRSA was significantly more prevalent during the AMR surveillance compared to the previous year [14 (66.7%) to 29 (76.3%); P = 0.046]. During the AMR surveillance, MRSA more frequently originated from medical departments compared to intensive care unit, surgical department, and internal medicine (P = 0.027) indicating increasing MRSA infections in patients with less severe clinical condition and no apparent risk factors. Higher prevalence of MRSA and its lower susceptibility to erythromycin were revealed by implementation of active AMR surveillance, which may reflect more thoughtful collection of bloodstream samples from patients with suspected BSI.

show abstract

“…In the past, microbial surveillance systems have improved infection control by helping acquire knowledge concerning risk factors, trends in resistance, and how to improve therapeutics (41). A recently published international expert opinion on the management of infection due to azole-resistant A. fumigatus recommends reconsidering azole monotherapy in regions where azole resistance rates exceed 10% (42).…”

Section: Discussionmentioning

confidence: 99%

Culture-Based Methods and Molecular Tools for Azole-Resistant Aspergillus fumigatus Detection in a Belgian University Hospital

et al. 2017

View full text Add to dashboard Cite

Azole-resistant Aspergillus fumigatus is an increasing worldwide problem with major clinical implications. Surveillance is warranted to guide clinicians to provide optimal treatment to patients. To investigate azole resistance in clinical Aspergillus isolates in our institution, a Belgian university hospital, we conducted a laboratory-based surveillance between June 2015 and October 2016. Two different approaches were used: a prospective culture-based surveillance using VIPcheck on unselected A. fumigatus (n ϭ 109 patients, including 19 patients with proven or probable invasive aspergillosis [IA]), followed by molecular detection of mutations conferring azole resistance, and a retrospective detection of azole-resistant A. fumigatus in bronchoalveolar lavage fluid using the commercially available AsperGenius PCR (n ϭ 100 patients, including 29 patients with proven or probable IA). By VIPcheck, 25 azole-resistant A. fumigatus specimens were isolated from 14 patients (12.8%). Of these 14 patients, only 2 had proven or probable IA (10.5%). Mutations at the cyp51A gene were observed in 23 of the 25 A. fumigatus isolates; TR 34 /L98H was the most prevalent mutation (46.7%), followed by TR 46 / Y121F/T289A (26.7%). Twenty-seven (27%) patients were positive for the presence of Aspergillus species by AsperGenius PCR. A. fumigatus was detected by AsperGenius in 20 patients, and 3 of these patients carried cyp51A mutations. Two patients had proven or probable IA and cyp51A mutation (11.7%). Our study has shown that the detection of azole-resistant A. fumigatus in clinical isolates was a frequent finding in our institution. Hence, a rapid method for resistance detection may be useful to improve patient management. Centers that care for immunocompromised patients should perform routine surveillance to determine their local epidemiology.KEYWORDS VIPcheck, AsperGenius, cyp51A, cyp51B, hapE A zole resistance in Aspergillus fumigatus is an emerging problem worldwide, with major epidemiological and clinical implications (1-6). Mold active triazoles are commonly used as first line treatment and prophylaxis of invasive aspergillosis (IA) (7). Mutations in the cyp51A gene, which encodes the target of azoles, the lanosterol 14␣-demethylase, represent the most commonly reported mechanism conferring azole resistance and consequently treatment failure in A. fumigatus (8-10). The most prevalent mutation (TR 34 /L98H) involves the insertion of a 34-bp tandem repeat (TR 34 ) in the promoter region of the cyp51A gene and a point mutation within the gene leading to an amino acid substitution (L98H). More recently, an additional cyp51A-mediated resistant genotype (TR 46 /Y121F/T289A) has been reported: a 46-bp tandem repeat (TR 46 ) in the promoter of cyp51A gene and two point mutations within the gene

show abstract

Integrated Multilevel Surveillance of the World's Infecting Microbes and Their Resistance to Antimicrobial Agents

Cited by 78 publications

References 125 publications

Epidemiology and Molecular Characterization of Macrolide-Resistant Streptococcus pyogenes in Taiwan

Epidemiology and Molecular Characterization of Macrolide-Resistant Streptococcus pyogenes in Taiwan

Differences in MRSA prevalence and resistance patterns in a tertiary center before and after joining an international program for surveillance of antimicrobial resistance

Culture-Based Methods and Molecular Tools for Azole-Resistant Aspergillus fumigatus Detection in a Belgian University Hospital

Contact Info

Product

Resources

About