Integrated Neurophotonics: Toward Dense Volumetric Interrogation of Brain Circuit Activity—at Depth and in Real Time

Moreaux, Laurent; Yatsenko, Dimitri; Sacher, Wesley D.; Choi, Jaebin; Lee, Changhyuk; Kubat, Nicole J.; Cotton, R.; Boyden, Edward S.; Lin, Michael Z.; Tian, Lin; Tolias, Andreas S.; Poon, Joyce K. S.; Shepard, Kenneth L.; Roukes, M. L.

doi:10.1016/j.neuron.2020.09.043

Cited by 53 publications

(39 citation statements)

References 175 publications

(214 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In October 2020, Moreaux, Yatsenko, and Sacher, et al, proposed what they called a "new paradigm" for brain imaging [51]. Unlike the clunky and often disputed significance of functional magnetic resonance imaging scans (fMRI), Moreaux and his team proposed the implantation of neurophotonics deep in the brain which would be capable of recording neuronal activity at the scale of the single cell and in real time.…”

Section: The Neurochemical Brainmentioning

confidence: 99%

The Neuropolitics of Brain Science and Its Implications for Human Enhancement and Intellectual Property Law

2020

View full text Add to dashboard Cite

As we learn more about how the brain functions, the study of the brain changes what we know about human creativity and innovation and our ability to enhance the brain with technology. The possibilities of direct brain-to-brain communication, the use of cognitive enhancing drugs to enhance human intelligence and creativity, and the extended connections between brains and the larger technological world, all suggest areas of linkage between intellectual property (IP) law and policy and the study of the brain science. Questions of importance include: Who owns creativity in such a world when humans are enhanced with technology? And how does one define an original work of authorship or invention if either were created with the aid of an enhancement technology? This paper suggests that new conceptualizations of the brain undermine the notion of the autonomous individual and may serve to locate creativity and originality beyond that of individual creation. In this scenario, the legal fiction of individual ownership of a creative work will be displaced, and as this paper warns, under current conditions the IP policies which may take its place will be of concern absent a rethinking of human agency in the neuropolitical age.

show abstract

Section: The Neurochemical Brainmentioning

confidence: 99%

The Neuropolitics of Brain Science and Its Implications for Human Enhancement and Intellectual Property Law

2020

View full text Add to dashboard Cite

show abstract

“…To achieve imaging at depth, these instruments also require implantation of their requisite GRIN lenses (typically, with 0.3–2 mm diameter); this results in even greater displacement of brain tissue while still delivering only restricted fields of view. The limitations with existing state-of-the-art imaging approaches at depth have led to the search for alternatives in which the imager itself is given a shank form factor and directly inserted into the tissue 9 , 10 .…”

Section: Introductionmentioning

confidence: 99%

Toward implantable devices for angle-sensitive, lens-less, multifluorescent, single-photon lifetime imaging in the brain using Fabry–Perot and absorptive color filters

et al. 2022

Self Cite

View full text Add to dashboard Cite

Implantable image sensors have the potential to revolutionize neuroscience. Due to their small form factor requirements; however, conventional filters and optics cannot be implemented. These limitations obstruct high-resolution imaging of large neural densities. Recent advances in angle-sensitive image sensors and single-photon avalanche diodes have provided a path toward ultrathin lens-less fluorescence imaging, enabling plenoptic sensing by extending sensing capabilities to include photon arrival time and incident angle, thereby providing the opportunity for separability of fluorescence point sources within the context of light-field microscopy (LFM). However, the addition of spectral sensitivity to angle-sensitive LFM reduces imager resolution because each wavelength requires a separate pixel subset. Here, we present a 1024-pixel, 50 µm thick implantable shank-based neural imager with color-filter-grating-based angle-sensitive pixels. This angular-spectral sensitive front end combines a metal–insulator–metal (MIM) Fabry–Perot color filter and diffractive optics to produce the measurement of orthogonal light-field information from two distinct colors within a single photodetector. The result is the ability to add independent color sensing to LFM while doubling the effective pixel density. The implantable imager combines angular-spectral and temporal information to demix and localize multispectral fluorescent targets. In this initial prototype, this is demonstrated with 45 μm diameter fluorescently labeled beads in scattering medium. Fluorescent lifetime imaging is exploited to further aid source separation, in addition to detecting pH through lifetime changes in fluorescent dyes. While these initial fluorescent targets are considerably brighter than fluorescently labeled neurons, further improvements will allow the application of these techniques to in-vivo multifluorescent structural and functional neural imaging.

show abstract

“…At present, the real-time direct monitoring of large-scale neuronal activity can truly revolutionize brain research. Integrated neurophotonics has been introduced for dense volumetric imaging of brain circuit activity at deep brain has been proposed [ 168 ]. Hand-held MSOT technology was recently employed for the characterization of hemodynamic changes related with neuronal activity induced by optogenetic stimulation of intra-cortical connections in the rat brain [ 169 ].…”

Section: Introductionmentioning

confidence: 99%

Multi-scale optoacoustic molecular imaging of brain diseases

Razansky

Klohs

2021

Eur J Nucl Med Mol Imaging

View full text Add to dashboard Cite

The ability to non-invasively visualize endogenous chromophores and exogenous probes and sensors across the entire rodent brain with the high spatial and temporal resolution has empowered optoacoustic imaging modalities with unprecedented capacities for interrogating the brain under physiological and diseased conditions. This has rapidly transformed optoacoustic microscopy (OAM) and multi-spectral optoacoustic tomography (MSOT) into emerging research tools to study animal models of brain diseases. In this review, we describe the principles of optoacoustic imaging and showcase recent technical advances that enable high-resolution real-time brain observations in preclinical models. In addition, advanced molecular probe designs allow for efficient visualization of pathophysiological processes playing a central role in a variety of neurodegenerative diseases, brain tumors, and stroke. We describe outstanding challenges in optoacoustic imaging methodologies and propose a future outlook.

show abstract

Integrated Neurophotonics: Toward Dense Volumetric Interrogation of Brain Circuit Activity—at Depth and in Real Time

Cited by 53 publications

References 175 publications

The Neuropolitics of Brain Science and Its Implications for Human Enhancement and Intellectual Property Law

The Neuropolitics of Brain Science and Its Implications for Human Enhancement and Intellectual Property Law

Toward implantable devices for angle-sensitive, lens-less, multifluorescent, single-photon lifetime imaging in the brain using Fabry–Perot and absorptive color filters

Multi-scale optoacoustic molecular imaging of brain diseases

Contact Info

Product

Resources

About