Integrated Physiological and Transcriptomic Analyses Revealed Improved Cold Tolerance in Cucumber (Cucumis sativus L.) by Exogenous Chitosan Oligosaccharide

Tan, Chong; Li, Na; Wang, Yidan; Yang, Lu; Cao, Ruifang; Ye, Xueling

doi:10.3390/ijms24076202

Cited by 16 publications

(7 citation statements)

References 84 publications

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“…In addition, cinnamic acid was rapidly converted to trans-2-hydroxycinnamate under the catalysis of cinnamate 2-hydroxylase, followed by the generation of coumarine through the upregulation of β-glucosidase. Studies have shown that upregulation of PAL , C4H , and 4CL in plants can increase plant tolerance to biotic or abiotic stress [ 49 , 50 ]. Among them, overexpression of PAL has been proven to reduce the level of ROS in Arabidopsis seeds and leaves, increase the activity of POD, CAT, and the content of flavonoids, and reduce the accumulation of MDA [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

Biochar-Dual Oxidant Composite Particles Alleviate the Oxidative Stress of Phenolic Acid on Tomato Seed Germination

Shen

Peng

et al. 2023

Antioxidants

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Phenolic acid is a well-known allelochemical, but also a pollutant in soil and water impeding crop production. Biochar is a multifunctional material widely used to mitigate the phenolic acids allelopathic effect. However, phenolic acid absorbed by biochar can still be released. In order to improve the removal efficiency of phenolic acids by biochar, the biochar-dual oxidant (BDO) composite particles were synthesized in this study, and the underlying mechanism of the BDO particles in ameliorating p-coumaric acid (p-CA) oxidative damage to tomato seed germination was revealed. Upon p-CA treatment, the BDO composite particles application increased the radical length, radical surface area, and germination index by 95.0%, 52.8%, and 114.6%, respectively. Compared to using biochar or oxidants alone, the BDO particles addition resulted in a higher removal rate of p-CA and produced more O2•−, HO•, SO4•− and 1O2 radicals via autocatalytic action, suggesting that BDO particles removed phenolic acid by both adsorption and free radical oxidation. The addition of BDO particles maintained the levels of the antioxidant enzyme activity close to the control, and reduced the malondialdehyde and H2O2 by 49.7% and 49.5%, compared to the p-CA treatment. Integrative metabolomic and transcriptomic analyses revealed that 14 key metabolites and 62 genes were involved in phenylalanine and linoleic acid metabolism, which increased dramatically under p-CA stress but down-regulated with the addition of BDO particles. This study proved that the use of BDO composite particles could alleviate the oxidative stress of phenolic acid on tomato seeds. The findings will provide unprecedented insights into the application and mechanism of such composite particles as continuous cropping soil conditioners.

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Section: Discussionmentioning

confidence: 99%

Biochar-Dual Oxidant Composite Particles Alleviate the Oxidative Stress of Phenolic Acid on Tomato Seed Germination

Shen

Peng

et al. 2023

Antioxidants

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“…Incorporating chitosan oligosaccharide in the study amplified the cold stress response in cucumber seedlings. RNA sequencing data unveiled the expression profiles of CsCCO gene under these conditions [ 33 ]. Notably, genes such as CsNCED6, CsNCED5b, CsNCED5a, CsCCD8, CsCCD7, CsNCED2, CsCCDL-a and CsCCD4b exhibited activation or upregulation in response to cold stress and chitosan oligosaccharide treatment.…”

Section: Discussionmentioning

confidence: 99%

“…The expression analysis of all CCO genes to enhance cold tolerance in cucumber was performed by applying chitosan oligosaccharide through a spray method. This analysis utilized previously gathered high-throughput sequencing data [ 33 ].…”

Section: Methodsmentioning

confidence: 99%

Genome-wide identification of CCO gene family in cucumber (Cucumis sativus) and its comparative analysis with A. thaliana

Akram,

Siddique,

Shafiq

et al. 2023

BMC Plant Biol

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Carotenoid cleavage oxygenase (CCO) is an enzyme capable of converting carotenoids into volatile, aromatic compounds and it plays an important role in the production of two significant plant hormones, i.e., abscisic acid (ABA) and strigolactone (SL). The cucumber plant genome has not been mined for genomewide identification of the CCO gene family. In the present study, we conducted a comprehensive genome-wide analysis to identify and thoroughly examine the CCO gene family within the genomic sequence of Cucumis sativus L. A Total of 10 CCO genes were identified and mostly localized in the cytoplasm and chloroplast. The CCO gene is divided into seven subfamilies i.e. 3 NCED, 3 CCD, and 1 CCD-like (CCDL) subfamily according to phylogenetic analysis. Cis-regulatory elements (CREs) analysis revealed the elements associated with growth and development as well as reactions to phytohormonal, biotic, and abiotic stress conditions. CCOs were involved in a variety of physiological and metabolic processes, according to Gene Ontology annotation. Additionally, 10 CCO genes were regulated by 84 miRNA. The CsCCO genes had substantial purifying selection acting upon them, according to the synteny block. In addition, RNAseq analysis indicated that CsCCO genes were expressed in response to phloem transportation and treatment of chitosan oligosaccharides. CsCCD7 and CsNCED2 showed the highest gene expression in response to the exogenous application of chitosan oligosaccharides to improve cold stress in cucumbers. We also found that these genes CsCCD4a and CsCCDL-a showed the highest expression in different plant organs with respect to phloem content. The cucumber CCO gene family was the subject of the first genome-wide report in this study, which may help us better understand cucumber CCO proteins and lay the groundwork for the gene family's future cloning and functional investigations.

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“…Soil amendment with chitosan enhanced leaf photosynthesis, leaf biomass, gas exchange, and chlorophyll fluorescence in lettuce [252]. Chitosan application reduced damage caused by cold stress in cucumber seedlings [253]. The application of chitosan and biochar was reported to improve wheat's biochemical, physiological, anatomical, and morphological traits under drought stress [254].…”

Section: Chitosanmentioning

confidence: 99%

Recent Advancements in Mitigating Abiotic Stresses in Crops

Oyebamiji,

Adigun,

Shamsudin

et al. 2024

Horticulturae

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In recent years, the progressive escalation of climate change scenarios has emerged as a significant global concern. The threat to global food security posed by abiotic stresses such as drought, salinity, waterlogging, temperature stress (heat stress, freezing, and chilling), and high heavy metal accumulation is substantial. The implementation of any of these stresses on agricultural land induces modifications in the morphological, biochemical, and physiological processes of plants, leading to diminished rates of germination, growth, photosynthesis, respiration, hormone and enzyme activity disruption, heightened oxidative stress, and ultimately, a reduction in crop productivity. It is anticipated that the frequency of these stresses will progressively escalate in the future as a result of a rise in climate change events. Therefore, it is crucial to develop productive strategies to mitigate the adverse effects of these challenges on the agriculture industry and improve crop resilience and yield. Diverse strategies have been implemented, including the development of cultivars that are resistant to climate change through the application of both conventional and modern breeding techniques. An additional application of the prospective and emerging technology of speed breeding is the acceleration of tolerance cultivar development. Additionally, plant growth regulators, osmoprotectants, nutrient and water management, planting time, seed priming, microbial seed treatment, and arbuscular mycorrhiza are regarded as effective methods for mitigating abiotic stresses. The application of biochar, kaolin, chitosan, superabsorbent, yeast extract, and seaweed extract are examples of promising and environmentally benign agronomic techniques that have been shown to mitigate the effects of abiotic stresses on crops; however, their exact mechanisms are still not yet fully understood. Hence, collaboration among researchers should be intensified to fully elucidate the mechanisms involved in the action of the emerging technologies. This review provides a comprehensive and current compilation of scientific information on emerging and current trends, along with innovative strategies to enhance agricultural productivity under abiotic stress conditions.

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Integrated Physiological and Transcriptomic Analyses Revealed Improved Cold Tolerance in Cucumber (Cucumis sativus L.) by Exogenous Chitosan Oligosaccharide

Cited by 16 publications

References 84 publications

Biochar-Dual Oxidant Composite Particles Alleviate the Oxidative Stress of Phenolic Acid on Tomato Seed Germination

Biochar-Dual Oxidant Composite Particles Alleviate the Oxidative Stress of Phenolic Acid on Tomato Seed Germination

Genome-wide identification of CCO gene family in cucumber (Cucumis sativus) and its comparative analysis with A. thaliana

Recent Advancements in Mitigating Abiotic Stresses in Crops

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