Integrated proteomics and metabolomics for dissecting the mechanism of global responses to salt and alkali stress in Suaeda corniculata

Abstract

Background and aims Soil alkalinity and salinity are two of the most common environmental stress factors that impact plant growth and productivity. S. corniculata is native to the saline-alkali soil of Northeast China which shows a higher alkali tolerance than other suaeda plants. It will be very important to identify the effect of salt and alkali stress on S. corniculata through proteomics and metabolomics analysis to improve understanding of the resistance of plants. Methods S. corniculata seedlings were exposed to salt and alkali stress for 72 h, respectively. Metabolic changes were quantified by conducting MS-based proteomics and NMR-based metabolomics analysis.Results The response of S. corniculata to salt stress was distinct from that of plants subjected to alkali stress at both physiological and molecular levels. The integrated-omic studies identified 22 and 19 differentially expressed proteins and metabolites mainly involved in carbohydrate metabolism and amino acid metabolism. Conclusions On account of the up-regulation of energy metabolism and higher accumulation of organic osmolytes, S. corniculata shows high pH resistance when it suffers alkali stress. These findings provide insights into the different regulatory mechanisms in halophyte S. corniculata response to salt and alkali stresses.

Metabolomics Analysis Reveals the Alkali Tolerance Mechanism in Puccinellia tenuiflora Plants Inoculated with Arbuscular Mycorrhizal Fungi

“…The application of metabolomics methods offers a rational way to reveal the implications of these metabolic changes on a broad scale [31]. Currently, metabolomics-based technologies have been broadly employed to clarify the metabolic responses of various plants (both halophytes and glycophytes) to salt stress, such as Hordeum vulgare [32], Oryza sativa [33], Suaeda corniculata [34], Glycine max [30], and Zea mays [35]. These studies accelerate the understanding of the molecular mechanisms of plant salt tolerance at the metabolic level.…”

Isobaric tags for relative and absolute quantification‐based proteomic analysis of Puccinellia tenuiflora inoculated with arbuscular mycorrhizal fungi reveal stress response mechanisms in alkali‐degraded soil

“…Proteomics have been used as a useful tool for facilitating the comparison of complex mixtures of proteins, and it is now widely applied for exploring the molecular mechanisms of plants in response to environmental stresses (Lv et al, ). Recently, proteomics‐based technologies have been broadly employed to identify the proteins responsible for salinity tolerance in halophytes such as Suaeda corniculata (Pang, Zhang, Zang, Wei, & Yan, ), Halogeton glomeratus (Wang, Yao, Li, Meng, & Ma, ), and Tangut nitraria (Cheng et al, ), which has accelerated the understanding of the protein‐level molecular mechanisms of plant salt tolerance. However, the effect of AMF on salinity stress tolerance in halophytes has not been explored using proteomics.…”

Proteomic and Metabolomic Analyses of the Inflorescences Axis of Castor (Ricinus conununis L.) at Different Developmental Stages

“…Castor plants bear, separate male and female owers on the same individual; a castor haplometrotic in orescence bears only female owers, and a castor monoecious in orescence bears both female and male owers [4][5][6][7][8]. The Lm female line castor, which is cultivated by the Tongliao Agricultural Science Institution, is used in the hybridization of species and strains as a male sterility line or maintainer line.…”