Integrated transcriptome and metabolite profiling highlights the role of benzoxazinoids in wheat resistance against Fusarium crown rot

Duan, Shuonan; Jin, Jingjing; Gao, Yutian; Jin, Changlin; Mu, Junyi; Zhen, Wenchao; Sun, Qixin; Xie, Chaojie; Ma, Jun

doi:10.1016/j.cj.2021.06.004

Cited by 20 publications

(16 citation statements)

References 68 publications

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“…They are involved in both aerial and below-ground defense against pests and diseases. Though we could not verify the amounts of BX accumulation due to the absence of authentic standards, reports on wheat and maize have demonstrated that transcript abundance results in their high accumulation and defense against diseases and pests (Duan et al, 2021;Sue et al, 2021).…”

Section: Activated Diterpene Synthases and Bx Genes Induce Phytoalexi...mentioning

confidence: 81%

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Hrip1 mediates rice cell wall fortification and phytoalexins elicitation to confer immunity against Magnaporthe oryzae

Ninkuu

Yan²,

Zhang³

et al. 2022

Front. Plant Sci.

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Magnaporthe oryzae is a potent fungus that adversely affects rice yield. Combinatorial techniques of prevention, toxic chemicals, and fungicide are used to remedy rice blast infection. We reported the role of Hrip1 in cell death elicitation and expression of systematic acquired resistance that could potentially stifle M. oryzae infection. In this study, transcriptome and metabolomic techniques were used to investigate the mechanism by which Hrip1 reprogramed the transcriptome of rice seedlings to confer immunity against M. oryzae. Our results showed that Hrip1 induces cell wall thickening and phytoalexin elicitation to confer immunity against M. oryzae infection. Hrip1 activates key lignin biosynthetic genes and myeloblastosis transcription factors that act as molecular switches for lignin production. Lignin content was increased by 68.46% and more after 48 h onwards in Hrip1-treated seedlings compared to the control treatment. Further analysis of cell wall morphology using the transmission electron microscopy technique revealed over 100% cell wall robustness. Hrip1 also induced the expression of 24 diterpene synthases. These include class I and II terpene synthases, cytochrome P450 subfamilies (OsCYP76M and OsCYP71Z), and momilactones synthases. The relationship between the expression of these genes and metabolic elicitation was analyzed using ultra-performance liquid chromatography–tandem mass spectrometry. Enhanced amounts of momilactones A and B, oryzalactone, and phytocassane A and G were detected in the Hrip1-treated leaves. We also identified seven benzoxazinoid genes (BX1-BX7) that could improve rice immunity. Our findings show that Hrip1 confers dual immunity by leveraging lignin and phytoalexins for physical and chemical resistance. This study provides novel insights into the mechanisms underlying Hrip1-treated plant immunity.

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Section: Activated Diterpene Synthases and Bx Genes Induce Phytoalexi...mentioning

confidence: 81%

“…Hrip1 activated seven BXs genes in the rice genome. Although we could not quantify these metabolites due to the absence of authentic standards, reports on wheat and maize showed that transcript abundance of BX genes results in high BX glycoside accumulation (Duan et al, 2021;Sue et al, 2021).…”

Section: Discussionmentioning

confidence: 96%

Hrip1 mediates rice cell wall fortification and phytoalexins elicitation to confer immunity against Magnaporthe oryzae

Ninkuu

Yan²,

Zhang³

et al. 2022

Front. Plant Sci.

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“…Phenyl, aryl, aliphatic rings, and aliphatic chain, often containing hydroxylated functionalities, can be bound to the parent phenolic ring (Figure 3). They contribute to various traits, such as pigmentation and resistance to pathogens in plants [132][133][134], and are generally present in food, spices, or food preservatives or belong to the list of generally recognized as safe (GRAS) compounds [135].…”

Section: Major Plant Phenolic Compounds and Their Effect On Fusariummentioning

confidence: 99%

“…This phenolic acid undergoes other enzymatic transformations, yielding a broad range of related phenylpropanoids [137] (Scheme 2). They contribute to various traits, such as pigmentation and resistance to pathogens in plants [132][133][134], and are generally present in food, spices, or food preservatives or belong to the list of generally recognized as safe (GRAS) compounds [135].…”

Section: Major Plant Phenolic Compounds and Their Effect On Fusariummentioning

confidence: 99%

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Bioprospecting Phenols as Inhibitors of Trichothecene-Producing Fusarium: Sustainable Approaches to the Management of Wheat Pathogens

Chtioui

Balmas

Delogu

et al. 2022

Toxins

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Fusarium spp. are ubiquitous fungi able to cause Fusarium head blight and Fusarium foot and root rot on wheat. Among relevant pathogenic species, Fusarium graminearum and Fusarium culmorum cause significant yield and quality loss and result in contamination of the grain with mycotoxins, mainly type B trichothecenes, which are a major health concern for humans and animals. Phenolic compounds of natural origin are being increasingly explored as fungicides on those pathogens. This review summarizes recent research activities related to the antifungal and anti-mycotoxigenic activity of natural phenolic compounds against Fusarium, including studies into the mechanisms of action of major exogenous phenolic inhibitors, their structure-activity interaction, and the combined effect of these compounds with other natural products or with conventional fungicides in mycotoxin modulation. The role of high-throughput analysis tools to decipher key signaling molecules able to modulate the production of mycotoxins and the development of sustainable formulations enhancing potential inhibitors’ efficacy are also discussed.

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A MYB family transcription factor TdRCA1 from wild emmer wheat regulates anthocyanin biosynthesis in coleoptile

Li,

Zhang,

et al. 2024

Theor Appl Genet

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Integrated transcriptome and metabolite profiling highlights the role of benzoxazinoids in wheat resistance against Fusarium crown rot

Cited by 20 publications

References 68 publications

Hrip1 mediates rice cell wall fortification and phytoalexins elicitation to confer immunity against Magnaporthe oryzae

Hrip1 mediates rice cell wall fortification and phytoalexins elicitation to confer immunity against Magnaporthe oryzae

Bioprospecting Phenols as Inhibitors of Trichothecene-Producing Fusarium: Sustainable Approaches to the Management of Wheat Pathogens

A MYB family transcription factor TdRCA1 from wild emmer wheat regulates anthocyanin biosynthesis in coleoptile

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