Integrated transcriptomic and metabolomic analyses revealed the regulatory mechanism of sulfur application in grain yield and protein content in wheat (Triticum aestivum L.)

Liu, Zhilian; Liu, Dongcheng; Fu, Xiaoyi; Du, Xiaodong; Zhang, Yuechen; Zhen, Wenchao; Li, Shan; Yang, Haichuan; He, Suqin; Li, Ruiqi

doi:10.3389/fpls.2022.935516

Cited by 3 publications

(1 citation statement)

References 67 publications

(70 reference statements)

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“…The identification of untargeted metabolites was conducted using UHPLC-MS/MS (ultra-performance liquid chromatography and tandem mass spectrometry) on a Vanquish UHPLC coupled with an Orbitrap Q Exactive TM HF mass spectrometer (Thermo Fisher, Dreieich, Germany) at Beijing Novogene Biotechnology Co., Ltd. (Beijing, China). The analytical conditions were consistent with those described in a previous study [55].…”

Section: Metabolome Analysissupporting

confidence: 63%

Transcriptomic and Metabolomic Analyses Reveal the Response to Short-Term Drought Stress in Bread Wheat (Triticum aestivum L.)

Fu,

Liu,

et al. 2024

Agronomy

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Drought stress, a major abiotic stress, significantly affects wheat (Triticum aestivum L.) production globally. To identify genes and metabolic pathways crucial for responding to short-term drought stress, we conducted transcriptomic and metabolomic analyses of winter wheat cultivar Jimai 418 at four developmental stages: jointing (GS31), booting (GS45), anthesis (GS65), and 8 days after anthesis (DAA8). Transcriptomic analysis identified 14,232 differentially expressed genes (DEGs) under drought stress compared to the control. Specifically, 1387, 4573, 7380, and 892 DEGs were identified at the four developmental stages, respectively. Enriched pathways associated with these DEGs included plant hormone signal transduction, mitogen-activated protein kinase (MAPK) signaling, galactose metabolism, and starch and sucrose metabolism. Totals of 222, 633, 358, and 38 differentially accumulated metabolites (DAMs) were identified at the four stages, respectively. Correlation analysis of both datasets revealed DEGs and DAMs associated with plant hormone signal transduction, arginine and proline metabolism, ABC transporters, and amino acid biosynthesis. These findings offer significant insights into Jimai 418’s molecular response to short-term drought stress. The identified DEGs, DAMs, and enriched pathways contribute to our understanding of wheat drought tolerance. This research will facilitate further investigations into drought tolerance mechanisms and guide the breeding of wheat varieties with enhanced drought resistance.

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Section: Metabolome Analysissupporting

confidence: 63%

Transcriptomic and Metabolomic Analyses Reveal the Response to Short-Term Drought Stress in Bread Wheat (Triticum aestivum L.)

Fu,

Liu,

et al. 2024

Agronomy

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Gene regulatory networks underlying sulfate deficiency responses in plants

Fernández,

Miño,

Canales

et al. 2024

Journal of Experimental Botany

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Sulfur (S) is an essential macronutrient for plants and its availability in soils is a relevant determinant for plant growth and development. Current regulatory policies aimed at reducing industrial S emissions and changes in agronomical practices have caused a decline in S contents in soils worldwide. Deficiency of sulfate — the primary form of S accessible to plants in soil — has adverse effects on both crop yield and nutritional quality. Therefore, recent research has increasingly focused on unraveling the molecular mechanisms through which plants detect and adapt to a limiting supply of sulfate. A significant part of these studies involves the use of omics technologies and has generated comprehensive catalogs of sulfate deficiency-responsive genes and processes, principally in Arabidopsis thaliana, with a few studies centering on crop species such as wheat, rice or members of the Brassica genus. Although we know that sulfate deficiency elicits an important reprogramming of the transcriptome, the transcriptional regulators orchestrating this response are not yet well understood. In this review, we summarize the knowledge on gene expression responses to sulfate deficiency and current efforts on the identification of transcription factors relevant for controlling this response. We further compare the transcriptional response and putative regulators between Arabidopsis thaliana and two relevant crop plants, rice and tomato, to gain insights into common mechanisms of response to sulfate deficiency.

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Combined Transcriptomic and Metabolomic Analyses Reveal the Mechanisms by Which the Interaction Between Sulfur and Nitrogen Affects Garlic Yield and Quality

Ren,

Pan,

Deng

et al. 2024

Horticulturae

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Nitrogen and sulfur are essential macronutrients in plant growth and development, and their interaction profoundly influences gene expression, metabolic activities, and adaptability in plants, directly affecting plant growth and yield. Garlic (Allium sativum L.) is a crop of significant economic and medicinal value. However, despite the critical role of the nitrogen–sulfur interaction in garlic’s adaptability, yield, and quality, the specific mechanisms underlying these effects remain unclear. In this study, transcriptomic and metabolomic analyses were employed to investigate the effects of combined sulfur and nitrogen application on garlic bulb tissues. The results show that the combined application of sulfur and nitrogen significantly increased the diameter and weight of garlic bulbs by 14.96% and 35.47%, respectively. The content of alliin increased by 28.48%, while the levels of abscisic acid (ABA), jasmonic acid (JA), salicylic acid (SA), and gibberellin (GA) increased by 15.82%, 12.94%, 32.34%, and 48.13%, respectively. Additionally, the activities of alliinase, superoxide dismutase (SOD), and catalase (CAT) were enhanced by 7.93%, 4.48%, and 19.74%, respectively. Moreover, the application of sulfur and nitrogen significantly reduced the malondialdehyde (MDA) content and peroxidase (POD) activity in garlic bulbs by 29.66% and 9.42%, respectively, thereby improving garlic’s adaptability and growth potential. Transcriptomic analysis revealed differentially expressed genes in several key pathways, including plant hormone signal transduction, RNA degradation, glutathione metabolism, amino acid biosynthesis, and glycerophospholipid metabolism. Metabolomic analysis identified 80 differentially abundant metabolites primarily consisting of amino acids, indole carboxylic acids, and fatty acids. The integrated transcriptomic and metabolomic analyses highlighted the pivotal roles of glutathione metabolism, glycerophospholipid metabolism, and amino acid biosynthesis pathways in the synergistic effects of sulfur and nitrogen. This study not only provides critical scientific evidence for understanding the mechanisms underlying the nitrogen–sulfur interaction’s impact on the yield and quality of garlic but also offers a scientific basis for optimizing nutrient management strategies to enhance garlic yield and quality.

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Integrated transcriptomic and metabolomic analyses revealed the regulatory mechanism of sulfur application in grain yield and protein content in wheat (Triticum aestivum L.)

Abstract: Integrated transcriptomic and metabolomic analyses revealed the regulatory mechanism of sulfur application in grain yield and protein content in wheat (Triticum aestivum L.). Front.

Cited by 3 publications

References 67 publications

Transcriptomic and Metabolomic Analyses Reveal the Response to Short-Term Drought Stress in Bread Wheat (Triticum aestivum L.)

Transcriptomic and Metabolomic Analyses Reveal the Response to Short-Term Drought Stress in Bread Wheat (Triticum aestivum L.)

Gene regulatory networks underlying sulfate deficiency responses in plants

Combined Transcriptomic and Metabolomic Analyses Reveal the Mechanisms by Which the Interaction Between Sulfur and Nitrogen Affects Garlic Yield and Quality

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