Integrated transcriptomic and metabolomics analysis reveals abscisic acid signal transduction and sugar metabolism pathways as defense responses to cold stress in Argyranthemum frutescens

Xu, Hongyuan; Li, Jingjing; Wang, Lijun; Li, Xiaoyü; Liu, Yanqing; Wang, Xuan; Gao, Tingting; Ma, Yueping

doi:10.1016/j.envexpbot.2022.105115

Cited by 22 publications

(16 citation statements)

References 91 publications

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“…Interestingly, we noticed that the soluble sugar content, ROS content, CAT activity and APX activity of WT were decreased after cold stress in this study. Further, we found similar results of soluble sugar content (Wang et al, 2022;Xu et al, 2023), ROS content (Wang et al, 2022;Shu et al, 2023) and antioxidative enzyme activity (Wang et al, 2022, Liu et al, Hao et al, 2022Liu et al, 2022) have been reported in previous studies. This results probably due to the impairment of the physiological metabolic sites of soluble sugar, ROS, CAT and APX by cold stress (Hao et al, 2022).…”

Section: Discussionsupporting

confidence: 90%

“…This results probably due to the impairment of the physiological metabolic sites of soluble sugar, ROS, CAT and APX by cold stress (Hao et al, 2022). In addition, the soluble sugar content, ROS content, CAT activity and APX activity of plants fluctuated under cold stress (Wang et al, 2022;Xu et al, 2023;Shu et al, 2023), rather than continuous increasing or decreasing, resulting in decreased content or activity of the physiological characteristics in a period of time after cold stress.…”

Section: Discussionmentioning

confidence: 99%

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Multi-omics approach reveals the contribution of OsSEH1 to rice cold tolerance

Jia

Zhang

et al. 2023

Front. Plant Sci.

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As low environmental temperature adversely affects the growth, development and geographical distribution, plants have evolved multiple mechanisms involving changing physiological and metabolic processes to adapt to cold stress. In this study, we revealed that nucleoporin-coding gene OsSEH1 was a positive regulator of cold stress in rice. Physiological assays showed that the activity of antioxidant enzymes showed a significant difference between osseh1 knock-out lines and wild type under cold stress. Metabolome analysis revealed that the contents of large-scale flavonoids serving as ROS scavengers were lower in osseh1 mutants compared with wild type under cold stress. Transcriptome analysis indicated that the DEGs between osseh1 knock-out lines and wild type plants were enriched in defense response, regulation of hormone levels and oxidation-reduction process. Integration of transcriptomic and metabolic profiling revealed that OsSEH1 plays a role in the oxidation-reduction process by coordinately regulating genes expression and metabolite accumulation involved in phenylpropanoid and flavonoid biosynthetic pathway. In addition, Exogenous ABA application assays indicated that osseh1 lines had hypersensitive phenotypes compared with wild type plants, suggesting that OsSEH1 may mediate cold tolerance by regulating ABA levels.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Multi-omics approach reveals the contribution of OsSEH1 to rice cold tolerance

Jia

Zhang

et al. 2023

Front. Plant Sci.

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“…Additionally, our results highlight the involvement of starch and sucrose metabolism, ABA transporters, and the MAPK signaling pathway in floral development, corroborating Wu et al‘s findings, where they treated the plants with cold stress and performed the transcriptome analysis [ 57 ]. Similar results were obtained by another research group who treated the Argyranthemum frutescens with cold stress [ 58 ]. Our targeted analysis of flowering-related gene ontology (GO) terms further facilitated a granular view of the regulatory landscape governing floral organ development.…”

Section: Discussionsupporting

confidence: 89%

Dynamic transcriptome analysis provides molecular insights into underground floral differentiation in Adonis Amurensis Regel & Radde

Xin,

Zhang,

Wang

et al. 2024

BMC Genom Data

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Understanding flower developmental processes is a prerequisite for improving flowering ‘plants’ production. Adonis amurensis is a fascinating spring ephemeral plant that develops its flower organs underground. Nevertheless, knowledge of the molecular mechanisms driving this particular process is scarce. Herein, we examined transcriptional changes during underground flower differentiation in A. amurensis and unveiled key differently regulated genes and pathways. High-throughput RNA sequencing of meristems at different flower developmental stages, including flower primordium (FP), sepal stage (SE), perianth primordium (PE), stamen stage (ST), and pistil stage (PI), identified 303,234 unigenes that showed 44.79% similarity with sequences in Aquilegia coerulea. Correlations, principal component, and differentially expressed genes (DEGs) analyses revealed that few molecular changes occurred during the transition from PE to ST. Many DEGs exhibited stage-specific regulations. Transcription factor (TF) and phytohormone family genes are critical regulators of the floral differentiation process in A. amurensis. The most differentially regulated TFs were MADS, FAR1, MYBs, AP2/ERF, B3, C2H2, and LOBs. We filtered out 186 candidate genes for future functional studies, including 18 flowering/circadian-related, 32 phytohormone-related, and TF family genes. Our findings deepen our understanding of the underground flower differentiation process and offer critical resources to dissect its regulatory network in A. amurensis. These findings establish a foundational platform for researchers dedicated to exploring the unique phenotypic characteristics of this specific flowering modality and delving into the intricate molecular mechanisms underpinning its regulation and expression.

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“…Studies have found that ABA can up-regulate the expression of PAL, C4H, CHS, chalcone lsomerase (CHI), and other key enzyme genes in plants, enhance phenylpropane metabolic pathway, and promote the synthesis of flavonoids [ 49 , 52 , 53 ]. In addition, some studies have also found that ABA can promote the expression of MYB-related transcription factors, and activate the binding of MYB to the promoters of downstream key enzyme genes to activate transcription [ 52 , 54 – 57 ]. Hamed et al found that when broad bean plants were given cycocel, ABA levels in the plants was increased both before and during flowering [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

“…ACC overexpression increased the content of malonyl-CoA. Increased ABA level up-regulates PAL and C4H gene expression, enhances phenylpropane metabolic pathway, increases 4-coumaroyl-CoA levels [ 61 ], activated the expression of related MYB transcription factors [ 52 , 57 ], and enhances the synthesis of flavonoid precursors [ 62 ]. All these provided sufficient substrates and enzymes for the synthesis of flavonoids in Hypericum attenuatum Choisy, and promoted the synthesis and accumulation of flavonoids by the organism using the substrates.…”

Section: Discussionmentioning

confidence: 99%

Effects of plant growth regulators on the contents of rutin, hyperoside and quercetin in Hypericum attenuatum Choisy

et al. 2023

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To explore the accumulation of rutin, hyperoside and quercetin in Hypericum attenuatum Choisy under treatment with different plant growth regulators, 100 mg/L, 200 mg/L and 300 mg/L cycocel, 100 mg/L, 200 mg/L and 300 mg/L mepiquat chloride and 1 mg/L, 2 mg/L and 3 mg/L naphthalene acetic acid were foliage sprayed on Hypericum attenuatum Choisy plants at the early growth stage. We sampled and determined the important flavonoid contents at the flowering stage. The results showed that the three plant growth regulators had different effects on the accumulation of rutin, hyperoside and quercetin in the leaves, stems and flowers of Hypericum attenuatum Choisy at the flowering stage. After spraying 1 mg/L naphthalene acetic acid at the early growth stage, the rutin contents in the leaves, stems and flowers increased by approximately 60.33%, 223.85% and 192.02%, respectively (P < 0.05). Spraying 100 mg/L mepiquat chloride increased the hyperoside contents in the leaves and flowers by approximately 7.77% and 12.87%, respectively (P < 0.05). Spraying 2 mg/L naphthalene acetic acid significantly increased the quercetin contents in the flowers and leaves by approximately 95.62% and 47.85%, respectively (P < 0.05). Therefore, at the early growth stage, spraying 1 mg/L naphthalene acetic acid significantly increased rutin content, spraying 100 mg/L mepiquat chloride significantly increased hyperoside content, and spraying 2 mg/L naphthalene acetic acid significantly increased quercetin content in Hypericum attenuatum Choisy. In conclusion, the accumulation of flavonoids in Hypericum attenuatum Choisy was regulated by plant growth regulators.

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Integrated transcriptomic and metabolomics analysis reveals abscisic acid signal transduction and sugar metabolism pathways as defense responses to cold stress in Argyranthemum frutescens

Cited by 22 publications

References 91 publications

Multi-omics approach reveals the contribution of OsSEH1 to rice cold tolerance

Multi-omics approach reveals the contribution of OsSEH1 to rice cold tolerance

Dynamic transcriptome analysis provides molecular insights into underground floral differentiation in Adonis Amurensis Regel & Radde

Effects of plant growth regulators on the contents of rutin, hyperoside and quercetin in Hypericum attenuatum Choisy

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