Integrated transcriptomics and metabolomics analyses provide insights into the mechanisms of capitulum architecture in Argyranthemum frutescens (Asteraceae)

Wang, Lijun; Li, Xiaoyü; Xu, Hongyuan; Li, Jingjing; Wang, Xuan; Liu, Yanqing; Zhao, Liang; Ma, Yueping

doi:10.1016/j.scienta.2023.112362

Cited by 3 publications

(1 citation statement)

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“…The sample extracts were analyzed based on the UPLC-ESI-MS/MS system. The analytical conditions were conducted as previously described [17,46,47], using a UPLC column, (Agilent SB-C18, 1.8 µm, 2.1 × 100 mm), with a mobile phase consisting of solvent A, which was pure water with 0.1% formic acid, and solvent B, which was acetonitrile with 0.1% formic acid. The gradient program was 95% A and 5% B at 0 min, a linear gradient to 5% A and 95% B within 9 min, then 5% A and 95% B for 1 min, and 95% A and 5% B adjusted within 0.1 min, and maintained for 2.9 min.…”

Section: Metabolite Extraction and Widely Targeted Metabolome Analysismentioning

confidence: 99%

Combined Widely Targeted Metabolomic, Transcriptomic, and Spatial Metabolomic Analysis Reveals the Potential Mechanism of Coloration and Fruit Quality Formation in Actinidia chinensis cv. Hongyang

Mao,

Gao,

Wang

et al. 2024

Foods

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Postharvest kiwifruit (Actinidia chinensis cv. Hongyang) pulp is mainly composed of outer yellow-flesh (LR) and inner red-flesh (HR). However, information about the differences in coloration and fruit quality between these two parts are limited. In this study, widely targeted metabolomic, transcriptomic, and spatial metabolomic analyses were used to reveal the potential mechanism of coloration and fruit quality formation. The results show that a total of 1001 metabolites were identified in Hongyang kiwifruit, and the accumulation of 211 metabolites were significantly higher in the HR than LR, including 69 flavonoids, 53 phenolic acids, and 38 terpenoids. There were no significant differences in the content of citric acid, quinic acid, glucose, fructose, or sucrose between the LR and HR. These results were consistent with the results from the RNA-seq profile and spatial metabolomic analysis. In addition, a total of 23 key candidate genes related to flesh color and fruit quality formation were identified and validated by qRT-PCR analysis. This study provides a theoretical basis for elucidating the underlying mechanism of the formation of kiwifruit flesh color and fruit quality.

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Section: Metabolite Extraction and Widely Targeted Metabolome Analysismentioning

confidence: 99%