Integrated view of molecular diagnosis and prognosis of dengue viral infection: future prospect of exosomes biomarkers

Rana, Rashmi; Kant, Ravi; Kaul, Dinesh; Sachdev, Anil; Ganguly, Nirmal Kumar

doi:10.1007/s11010-021-04326-8

Cited by 5 publications

(4 citation statements)

References 160 publications

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“…These therapies have found applications in the fields of regenerative medicine, pharmacology, and vaccinology, as shall be discussed in the following section. Moreover, the quantitative increase in circulating exosomes observed during viral infections often correlates with the severity of the disease, as illustrated by flavivirus infections, notably with Dengue virus [ 139 , 179 ]. Exosomes are thus biomarkers of viral disease progression.…”

Section: Exosomes and Biology Of Infectionmentioning

confidence: 99%

Exosome-Mediated Antigen Delivery: Unveiling Novel Strategies in Viral Infection Control and Vaccine Design

El Safadi,

Mokhtari,

Krejbich

et al. 2024

Vaccines

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Exosomes are small subtypes of extracellular vesicles (EVs) naturally released by different types of cells into their environment. Their physiological roles appear to be multiple, yet many aspects of their biological activities remain to be understood. These vesicles can transport and deliver a variety of cargoes and may serve as unconventional secretory vesicles. Thus, they play a crucial role as important vectors for intercellular communication and the maintenance of homeostasis. Exosome production and content can vary under several stresses or modifications in the cell microenvironment, influencing cellular responses and stimulating immunity. During infectious processes, exosomes are described as double-edged swords, displaying both beneficial and detrimental effects. Owing to their tractability, the analysis of EVs from multiple biofluids has become a booming tool for monitoring various pathologies, from infectious to cancerous origins. In this review, we present an overview of exosome features and discuss their particular and ambiguous functions in infectious contexts. We then focus on their properties as diagnostic or therapeutic tools. In this regard, we explore the capacity of exosomes to vectorize immunogenic viral antigens and their function in mounting adaptive immune responses. As exosomes provide interesting platforms for antigen presentation, we further review the available data on exosome engineering, which enables peptides of interest to be exposed at their surface. In the light of all these data, exosomes are emerging as promising avenues for vaccine strategies.

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Section: Exosomes and Biology Of Infectionmentioning

confidence: 99%

Exosome-Mediated Antigen Delivery: Unveiling Novel Strategies in Viral Infection Control and Vaccine Design

El Safadi,

Mokhtari,

Krejbich

et al. 2024

Vaccines

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show abstract

“…Infection with certain viral pathogens is frequently accompanied by an excessive release of cytokines. These include MERS-CoV, influenza virus (e.g., H1N1, H5N1), and hemorrhagic fevers (e.g., Dengue, Ebola, and Crimean-Congo virus infections) [ 125 , 126 , 127 , 128 , 129 ]. Different viruses are associated with diverse patterns of cytokine release, but so far, this has not been operationalized in terms of a comprehensive and universally effective therapeutic approach.…”

Section: Unleashing the Cytokine Cascadementioning

confidence: 99%

Cytokine Storm—Definition, Causes, and Implications

Jarczak

Nierhaus

2022

IJMS

118

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The human innate and adaptive immune systems consist of effector cells producing cytokines (interleukins, interferons, chemokines, and numerous other mediators). Usually, a fragile equilibrium of pro- and anti-inflammation effects is maintained by complex regulatory mechanisms. Disturbances of this homeostasis can lead to intricate chain reactions resulting in a massive release of cytokines. This may result in a drastic self-reinforcement of various feedback mechanisms, which can ultimately lead to systemic damage, multi-organ failure, or death. Not only pathogens can initiate such disturbances, but also congenital diseases or immunomodulatory therapies. Due to the complex and diverse interactions within the innate and adaptive immune systems, the understanding of this important clinical syndrome is incomplete to date and effective therapeutic approaches remain scarce.

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“…Particularly, exosomes, when genetically modified or amalgamated with materials [ 19 ], demonstrate enhanced therapeutic properties including active ingredient enrichment, targeted delivery, and overcoming physiological barriers to penetration [ 20 ]—traits not present in traditional singular products[ 21 , 22 ]. Moreover, exosomes serve diagnostic [ 23 ] and therapeutic purposes in wound-related applications, such as complex wound repair [ 24 ], graft success enhancement [ 25 ], treatment of related complications, and acting as diagnostic biomarkers [ 26 ]. Yet, the severely limited yield and propensity for loss of exosomes curtail their full potential realization.…”

Section: Introductionmentioning

confidence: 99%

Enhanced burn wound healing by controlled-release 3D ADMSC-derived exosome-loaded hyaluronan hydrogel

Zhu,

Hu,

Kong

et al. 2024

Regenerative Biomaterials

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Adipose mesenchymal stem cell (ADMSC)-derived exosomes (ADMSC-Exos) have shown great potential in regenerative medicine and been evidenced benefiting wound repair such as burns. However, the low yield, easy loss after direct coating, and no suitable loading system to improve their availability and efficacy hinder their clinical application for wound healing. And few studies focused on the comparison of biological functions between exosomes derived from different culture techniques, especially in exosome-releasing hydrogel system. Therefore, we designed a high-performance Exosome controllable releasing hydrogel system for burn wound healing, namely loading 3D printed microfiber culture derived exosomes in a highly biocompatible hyaluronic acid (HA). In this project, we compared the biological functions in vitro and in a burn model among exosomes derived from the conventional two-dimensional (2D) plate culture (2D-Exos), microcarrier culture (2.5D-Exos), and 3D printed microfiber culture (3D-Exos). Results showed that compared with 2D-Exos and 2.5D-Exos, 3D-Exos promoted HACATs and HUVECs cell proliferation and migration more significantly. Additionally, 3D-Exos had stronger angiogenesis-promoting effects in tube formation of (HUVECs) cells. Moreover, we found HA loaded 3D-Exos showed better burn wound healing promotion compared to 2D-Exos and 2.5D-Exos, including accelerated burn wound healing rate and better collagen remodeling. The study findings reveal that the hyaluronic acid (HA)-loaded, controllable-release 3D-Exos repair system distinctly augments therapeutic efficacy in terms of wound healing, while concurrently introducing a facile application approach. This system markedly bolsters the exosomal loading efficiency, provides a robust protective milieu, and potentiates the inherent biological functionalities of the exosomes. Our findings provide a rationale for more efficient utilization of high-quality and high-yield 3D exosomes in the future, and a novel strategy for healing severe burns.

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Integrated view of molecular diagnosis and prognosis of dengue viral infection: future prospect of exosomes biomarkers

Cited by 5 publications

References 160 publications

Exosome-Mediated Antigen Delivery: Unveiling Novel Strategies in Viral Infection Control and Vaccine Design

Exosome-Mediated Antigen Delivery: Unveiling Novel Strategies in Viral Infection Control and Vaccine Design

Cytokine Storm—Definition, Causes, and Implications

Enhanced burn wound healing by controlled-release 3D ADMSC-derived exosome-loaded hyaluronan hydrogel

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