Integrating chemotaxonomic-based metabolomics data with DNA barcoding for plant identification: A case study on south-east African Erythroxylaceae species

Alberts, P.S.F.; Meyer, J.J.M.

doi:10.1016/j.sajb.2021.10.005

Cited by 6 publications

(5 citation statements)

References 91 publications

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“…A DNA barcode was incorporated to address the challenge of chemical plasticity, as the genetic makeup of a particular species should be more stable under various environmental conditions [ 52 ]. In plants, the establishment and refinement of DNA barcodes have been more challenging due to the distinct genetic diversity among different species [ 53 ].…”

Section: Resultsmentioning

confidence: 99%

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Essential Oil Chemotypes and Genetic Variability of Cinnamomum verum Leaf Samples Commercialized and Cultivated in the Amazon

et al. 2022

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Cinnamomum verum (Lauraceae), also known as “true cinnamon” or “Ceylon cinnamon” has been widely used in traditional folk medicine and cuisine for a long time. The systematics of C. verum presents some difficulties due to genetic variation and morphological similarity between other Cinnamomum species. The present work aimed to find chemical and molecular markers of C. verum samples from the Amazon region of Brazil. The leaf EOs and the genetic material (DNA) were extracted from samples cultivated and commercial samples. The chemical composition of the essential oils from samples of C. verum cultivated (Cve1-Cve5) and commercial (Cve6-c-Cv9-c) was grouped by multivariate statistical analysis of Principal Component Analysis (PCA). The major compounds were rich in benzenoids and phenylpropanoids, such as eugenol (0.7–91.0%), benzyl benzoate (0.28–76.51%), (E)-cinnamyl acetate (0.36–32.1%), and (E)-cinnamaldehyde (1.0–19.73%). DNA barcodes were developed for phylogenetic analysis using the chloroplastic regions of the matK and rbcL genes, and psbA-trnH intergenic spacer. The psbA-trnH sequences provided greater diversity of nucleotides, and matK confirmed the identity of C. verum. The combination of DNA barcode and volatile profile was found to be an important tool for the discrimination of C. verum varieties and to examine the authenticity of industrial sources.

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Section: Resultsmentioning

confidence: 99%

“…Thus, developing a rapid and feasible method for the identification of Cinnamomum plants is needed to prevent their adulteration of trees [ 62 ]. DNA barcodes can be incorporated to address the challenge of chemical plasticity, as the genetic makeup of a particular species should be more stable under various environmental conditions [ 52 ].…”

Section: Resultsmentioning

confidence: 99%

Essential Oil Chemotypes and Genetic Variability of Cinnamomum verum Leaf Samples Commercialized and Cultivated in the Amazon

et al. 2022

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“…15, 16 Identification with these loci showed good results up to the genus level, although the species identified have undergone various growth or developmental stages. 12 This research provides novel information about purnajiwa species in Bali. Several studies have been conducted to optimize the amplification conditions in applying purnajiwa barcoding using COI and Ehoscn01a markers.…”

Section: Original Articlementioning

confidence: 96%

“…9,10 Several DNA barcodes, including internal transcribed spacer 2 (ITS2), psbA-trnH (intergenic spacer region), matK (maturase K), and rbcL (ribulose-1,5-bisphosphate carboxylase/ large oxygenase subunit) regions, are used to authenticate medicinal plants. 9,11, 12 The…”

Section: Introductionmentioning

confidence: 99%

Application of DNA Barcoding for authentication of Balinese traditional medicinal plant Purnajiwa (Kopsia arborea Blume. and Euchresta horsfieldii) (Lesch.) Benn

Ariati¹,

Sasadara

Wirawan

et al. 2022

Bali Med J.

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Background: Purnajiwa is one of the medicinal plants in Bali. Species information regarding this plant is still unconfirmed. Currently, this plant is considered rare and endangered. Molecular identification supports authentication at the species level, which will also support conservation efforts. Method: Molecular identification using DNA Barcoding was carried out on purnajiwa from three sampling locations in Bali (Jimbaran, Mambal, and Bedugul) using rbcL primers (rbcLaF and rbcLaR). Data analysis was performed using BLAST with species on GenBank. Pairwise and similarity values were used to measure the three samples' proximity to the Gen Bank species. Result: The phylogenetic tree was constructed with the Maximum Likelihood and Tamura-3-parameter model. The analysis results show a close relationship between the purnajiwa from Jimbaran and Mambal with the species Kopsia arborea (KP095079). Meanwhile, the Purnajiwa from Bedugul showed a difference from the other two purnajiwa and was closely related to Euchresta horsfieldii (U74225). Conclusion: It can be concluded that the Purnajiwa from Jimbaran and Mambal is a species of Kopsia arborea while the Purnajiwa from Bedugul is a species of Euchresta horsfieldii.

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“…Its role could be defined as the extension of traditional phytochemical investigation following the identification of biomarkers that can be statistically correlated to bioactivity changes but also as an attempt to understand complex plant mechanisms as a part of systems biology [ 1 ]. In several studies, metabolomics has been helpful in the fingerprinting of species, genotypes or ecotypes for taxonomic or biochemical (gene discovery) purposes [ 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 ]. For example, the PCA analysis of 1 H NMR metabolite fingerprinting was used to discriminate five Verbascum species.…”

Section: Introductionmentioning

confidence: 99%

Metabolomics as a Potential Chemotaxonomical Tool: Application on the Selected Euphorbia Species Growing Wild in Serbia

Sofrenić

Anđelković

Gođevac

et al. 2023

Plants

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Chemotaxonomy presents various challenges that need to be overcome in order to obtain valid and reliable results. Individual genetic and environmental variations can give a false picture and lead to wrong conclusions. Applying a holistic approach, based on multivariate data analysis, these challenges can be overcome. Thus, a metabolomics approach has to be optimized depending on the subject of research. We used 1H NMR-based metabolomics as a potential chemotaxonomic tool on the selected Euphorbia species growing wild in Serbia. Principal components analysis (PCA), soft independent modeling by class analogy (SIMCA) and Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS-DA) were used to analyze obtained NMR data in order to reveal chemotaxonomic biomarkers. The standard protocol for plant metabolomics was optimized aiming to extract more specific metabolites, which are characteristic for the Euphorbia genus. The obtained models were validated, which revealed that variables unique for each species were associated with certain classes of molecules according to literature data. In E. salicifolia, acacetin-7-O-glycoside (not found before in the species) was detected, and the structure of the aglycone part was solved based on 2D NMR data. In the presented paper, we have shown that metabolomics can be successfully used in Euphorbia chemotaxonomy.

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Integrating chemotaxonomic-based metabolomics data with DNA barcoding for plant identification: A case study on south-east African Erythroxylaceae species

Cited by 6 publications

References 91 publications

Essential Oil Chemotypes and Genetic Variability of Cinnamomum verum Leaf Samples Commercialized and Cultivated in the Amazon

Essential Oil Chemotypes and Genetic Variability of Cinnamomum verum Leaf Samples Commercialized and Cultivated in the Amazon

Application of DNA Barcoding for authentication of Balinese traditional medicinal plant Purnajiwa (Kopsia arborea Blume. and Euchresta horsfieldii) (Lesch.) Benn

Metabolomics as a Potential Chemotaxonomical Tool: Application on the Selected Euphorbia Species Growing Wild in Serbia

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