Integrating clinical data mining, network analysis and experimental validation reveal the anti-inflammatory mechanism of Huangqin Qingre Chubi Capsule in rheumatoid arthritis treatment

Hu, Yuedi; Liu, Jian; Qi, Yajun; Zhou, Qiao; Li, Yang; Cong, Chengzhi; Chen, Yiming

doi:10.1016/j.jep.2024.118077

Cited by 2 publications

(1 citation statement)

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“…ZL201310011369.8; Anhui medical system drug approval No. Z20050062), made up of the following eight traditional Chinese medicines: 31–33 Scutellaria baicalensis Georgi ( http://mpns.kew.org ), Gardenia jasminoides J.Ellis ( http://mpns.kew.org ), Clematis chinensis Osbeck ( http://mpns.kew.org ), Juglans regia L. ( http://mpns.kew.org ), Astragalus mongholicus Bunge ( http://mpns.kew.org ), Coix lacryma-jobi L. ( http://mpns.kew.org ), Tripterygium wilfordi i Hook. f. ( http://mpns.kew.org ), and Scolopendra subspinipes mutilans L. Koch ( https://db.ouryao.com ), combined in a ratio of 10:9:10:5:20:20:10:1.…”

Section: Introductionmentioning

confidence: 99%

Validation of Jianpi Qingre Tongluo Recipe in Reducing Inflammation and Dyslipidemia in Osteoarthritis via Lnc RNA HOTAIR/APN/PI3K/AKT

Chen,

Liu,

Wang

et al. 2024

IJGM

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Objective Jianpi Qingre Tongluo Recipe (JQP) has been widely used in clinical practice, and its anti-Osteoarthritis (OA) effectiveness and specific mechanism have been concerned. This study aims to explore the clinical effect of JQP in reducing inflammation and dyslipidemia in OA and the molecular mechanism. Methods The clinical efficacy of JQP in OA treatment was assessed through data mining. Through the network pharmacology technology, the interactive network of “active component-target-disease” was developed, the interaction relationship of the related proteins was analyzed, and enrichment analysis of gene pathway biological process was conducted. Molecular docking was carried out with PyMOL and AutodockTools-1.5.7. Finally, cell experiments were used to verify JQP’s delay of immune inflammation in OA. Results We found that JQP could ameliorate the immune inflammatory and lipid metabolism indicators; reduce VAS and SAS score in OA. A total of 98 genes overlapped between target genes of JQP and OA. TNF, IL-6, IL-1β, and AKT1 shared the highest centrality among all target genes. KEGG analysis unveiled that 98 intersection genes were predominantly enriched in PI3K/AKT pathway in the anti-OA system. In vitro, after peripheral blood mononuclear cell (PBMC) stimulation, inflammatory cytokines imbalances and the expressions of adiponectin (APN) were decreased in osteoarthritis-chondrocytes (OA-CH). Furthermore, JQP-containing serum protected OA-CHs through down-regulating HOTAIR levels, thereby up-regulating APN and depressing PI3K/AKT pathway. Conclusion This study suggests that JQP might reduce inflammation and improve lipid metabolism of OA by regulating HOTAIR/APN/PI3K/AKT. Our results bring a new solution for OA.

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Section: Introductionmentioning

confidence: 99%