Integrating eggshell-derived CaCO3/MgO nanocomposites and chitosan into a biomimetic scaffold for bone regeneration

Huang, Yazhuo; Ji, Yongrong; Kang, Zewen; Li, Fang; Ge, Shengfang; Yang, Da‐Peng; Ruan, Jing; Fan, Xianqun

doi:10.1016/j.cej.2020.125098

Cited by 76 publications

(51 citation statements)

References 49 publications

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“…Table 1 and Table 2 summarize the definition of the critical sized defect in the selected studies. In eight of the included papers Wistar Rats were used [ 5 , 6 , 12 , 13 , 14 , 15 , 16 , 17 ], six studies used Sprague-Dawley rats [ 8 , 18 , 19 , 20 , 21 , 22 ] and seven New Zealand rabbits [ 6 , 23 , 24 , 25 , 26 , 27 , 28 ]. The calvaria critical-sized bone defect was used in 90% of the included papers to assess new bone formation ( Table 1 and Table 2 ).…”

Section: Resultsmentioning

confidence: 99%

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The Influence of Eggshell on Bone Regeneration in Preclinical In Vivo Studies

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et al. 2020

Biology

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The aim of this study is to systemically review the available evidence on the in vivo behavior of eggshell as a guided bone regeneration substitute material. Five databases (PubMed, Cochrane, Web of Science, Scopus, EMBASE) were searched up to October 2020. In vivo animal studies with a bone defect model using eggshell as a grafting material were included. Risk of bias was assessed using SYRCLE tool and the quality assessment using the ARRIVE guidelines. Overall, a total of 581 studies were included in the study, 187 after duplicate removal. Using the inclusion and exclusion criteria 167 records were further excluded. The full text of the remaining 20 articles was assessed for eligibility and included in the qualitative and quantitative assessment synthesis. There were different methods of obtaining eggshell grafting materials. Eggshell is a biocompatible grafting material, with osteoconduction proprieties. It forms new bone similar to Bio-Oss and demineralized freeze-dried bone matrix. It can be combined with other materials to enhance its proprieties. Due to the high variability of the procedures, animals, production and assessment methods, no meta-analysis could be performed. Eggshell might be considered a promising biomaterial to be used in bone grafting procedures, though further research is needed.

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Section: Resultsmentioning

confidence: 99%

“…Follow-ups ranged from 2 to 24 weeks. A single observation interval was reported for eight of the included studies [ 5 , 6 , 12 , 13 , 15 , 22 , 23 , 28 ], while the rest had multiple observation points.…”

Section: Resultsmentioning

confidence: 99%

The Influence of Eggshell on Bone Regeneration in Preclinical In Vivo Studies

Opriș

Dinu

Băciuţ

et al. 2020

Biology

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“…It is noted that tunable osteogenesis and degradation rates are important for repairing the bone defect. Huang et al (2020) prepared a novel composite CaCO 3 /MgO/CMC/BMP2 scaffold with high mechanical strength, mineralization ability, biomimetic activity and osteogenic differentiation. The sustainable release of Mg 2+ and BMP2 played an important role in activating the phosphorylation of ERK1/2 and Akt pathways, which guided the osteogenesis formation using an in-situ rat calvarial defect model (Figure 6).…”

Section: Cs-based Hydrogels For Bone Regenerationmentioning

confidence: 99%

Section: Cs-based Hydrogelsmentioning

confidence: 99%

Recent Advances of Chitosan-Based Injectable Hydrogels for Bone and Dental Tissue Regeneration

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Tan

Zeng

et al. 2020

Front. Bioeng. Biotechnol.

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Traditional strategies of bone repair include autografts, allografts and surgical reconstructions, but they may bring about potential hazard of donor site morbidity, rejection, risk of disease transmission and repetitive surgery. Bone tissue engineering (BTE) is a multidisciplinary field that offers promising substitutes in biopharmaceutical applications, and chitosan (CS)-based bone reconstructions can be a potential candidate in regenerative tissue fields owing to its low immunogenicity, biodegradability, bioresorbable features, low-cost and economic nature. Formulations of CS-based injectable hydrogels with thermo/pH-response are advantageous in terms of their highwater imbibing capability, minimal invasiveness, porous networks, and ability to mold perfectly into an irregular defect. Additionally, CS combined with other naturally-derived or synthetic polymers and bioactive agents has proven to be an effective alternative to autologous bone and dental grafts. In this review, we will highlight the current progress in the development of preparation methods, physicochemical properties and applications of CS-based injectable hydrogels and their perspectives in bone and dental regeneration. We believe this review is intended as starting point and inspiration for future research effort to develop the next generation of tissue-engineering scaffold materials.

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“…Cell-based therapies are advanced therapeutic strategies that have brought promise for some severe diseases 7 - 9 . Unfortunately, cell-based therapy has still not become popularized in clinical applications.…”

Section: Introductionmentioning

confidence: 99%

Progenitor cell-derived exosomes endowed with VEGF plasmids enhance osteogenic induction and vascular remodeling in large segmental bone defects

et al. 2021

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Large segmental bone regeneration remains a great challenge due to the lack of vascularization in newly formed bone. Conventional strategies primarily combine bone scaffolds with seed cells and growth factors to modulate osteogenesis and angiogenesis. Nevertheless, cell-based therapies have some intrinsic issues regarding immunogenicity, tumorigenesis, bioactivity and off-the-shelf transplantation. Exosomes are nano-sized (50-200 nm) extracellular vesicles with a complex composition of proteins, nucleic acids and lipids, which are attractive as therapeutic nanoparticles for disease treatment. Exosomes also have huge potential as desirable drug/gene delivery vectors in the field of regenerative medicine due to their excellent biocompatibility and efficient cellular internalization. Methods: We developed a cell-free tissue engineering system using functional exosomes in place of seed cells. Gene-activated engineered exosomes were constructed by using ATDC5-derived exosomes to encapsulate the VEGF gene. The specific exosomal anchor peptide CP05 acted as a flexible linker and effectively combined the engineered exosome nanoparticles with 3D-printed porous bone scaffolds. Results: Our findings demonstrated that engineered exosomes play dual roles as an osteogenic matrix to induce the osteogenic differentiation of mesenchymal stem cells and as a gene vector to controllably release the VEGF gene to remodel the vascular system. In vivo evaluation further verified that the engineered exosome-mediated bone scaffolds could effectively induce the bulk of vascularized bone regeneration. Conclusion: In our current work, we designed specifically engineered exosomes based on the requirements of vascularized bone repair in segmental bone defects. This work simultaneously illuminates the potential of functional exosomes in acellular tissue engineering.

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Integrating eggshell-derived CaCO3/MgO nanocomposites and chitosan into a biomimetic scaffold for bone regeneration

Cited by 76 publications

References 49 publications

The Influence of Eggshell on Bone Regeneration in Preclinical In Vivo Studies

The Influence of Eggshell on Bone Regeneration in Preclinical In Vivo Studies

Recent Advances of Chitosan-Based Injectable Hydrogels for Bone and Dental Tissue Regeneration

Progenitor cell-derived exosomes endowed with VEGF plasmids enhance osteogenic induction and vascular remodeling in large segmental bone defects

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