Integrating graphene oxide, functional DNA and nucleic-acid-manipulating strategies for amplified biosensing

Liu, Meng; Zhang, Wenqing; Chang, Dingran; Zhang, Qiang; Brennan, John D.; Li, Yingfu

doi:10.1016/j.trac.2015.03.027

Cited by 37 publications

(19 citation statements)

References 89 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since its discovery, graphene has been widely used in bioassays [269-271]. GO is an extraordinary optical sensing material that can serve either as an energy acceptor or as an energy donor for a fluorophore.…”

Section: Carbon Materials-based Fluorescent Nanoprobes For Sensingmentioning

confidence: 99%

Fluorescent nanoprobes for sensing and imaging of metal ions: Recent advances and future perspectives

et al. 2016

View full text Add to dashboard Cite

Summary Recent advances in nanoscale science and technology have generated nanomaterials with unique optical properties. Over the past decade, numerous fluorescent nanoprobes have been developed for highly sensitive and selective sensing and imaging of metal ions, both in vitro and in vivo. In this review, we provide an overview of the recent development of the design and optical properties of the different classes of fluorescent nanoprobes based on noble metal nanomaterials, upconversion nanoparticles, semiconductor quantum dots, and carbon-based nanomaterials. We further detail their application in the detection and quantification of metal ions for environmental monitoring, food safety, medical diagnostics, as well as their use in biomedical imaging in living cells and animals.

show abstract

Section: Carbon Materials-based Fluorescent Nanoprobes For Sensingmentioning

confidence: 99%

Fluorescent nanoprobes for sensing and imaging of metal ions: Recent advances and future perspectives

et al. 2016

View full text Add to dashboard Cite

show abstract

“…[14] Furthermore, both AB and MB are widely available commercially with diverse surface chemistries for attachment. [15] rGO was examined because of its large surface area, its ability to function as an effective quencher for fluorescence, and its ability to adsorb single-stranded DNA molecules nonspecifically, [16] which could provide a simple way to immobilize F-EC1.…”

Section: Evaluation Of Different Surfaces For Immobilization Of F-ec1mentioning

confidence: 99%

“…After multiple washes,~3.1 pmol remained attached to the surface. Reduced graphene oxide has π-rich conjugation domains and can adsorb single-stranded nucleic acid molecules, such as DNAzymes, via π-π stacking interactions [16] so the version of F-EC1 used in the rGO experiment was not biotinylated.…”

Section: Evaluation Of Different Surfaces For Immobilization Of F-ec1mentioning

confidence: 99%

A Syringe‐Based DNAzyme Sensor for Bacterial Detection

et al. 2021

Self Cite

View full text Add to dashboard Cite

We present a simple approach for bacterial detection that uses a bead‐bound RNA‐cleaving DNAzyme (RcD) and a syringe. The RcD used in this study, F‐EC1, is fluorescently labelled and can be specifically activated by a protein target released specifically by E. coli. Upon immobilization on agarose beads, the kinetic behaviour of F‐EC1 remains similar to that in solution. The F‐EC1‐agarose system was able to detect as few as 1,000 CFUs of E. coli and retained high recognition specificity. A syringe‐based biosensor was subsequently designed, where F‐EC1‐bearing agarose beads were loaded onto a filter‐containing column. Upon incubation with an E. coli containing reaction mixture, the cleavage fragment released from the beads was filtered onto a nitrocellulose paper to be detected. The sensor was used to achieve the detection of E. coli in water, milk, and apple juice. This method is cost‐effective, simple to use, and can be adapted to other ligand‐responsive RNA‐cleaving DNAzymes.

show abstract

“…Another advantage of GO is the existence of the hydroxyl, carboxyl, epoxy groups and π rich conjugation domains on its surface that makes GO a suitable carrier for interacting with targeting agents and drugs. 23–25…”

Section: Introductionmentioning

confidence: 99%

“…Another advantage of GO is the existence of the hydroxyl, carboxyl, epoxy groups and p rich conjugation domains on its surface that makes GO a suitable carrier for interacting with targeting agents and drugs. [23][24][25] In this study, a dual-aptamer-functionalized graphene oxide (DAFGO) complex was designed using two kinds of aptamers (Sgc8c and ATP aptamers) and GO for identification of Molt-4 cells (T-cell line, ALL). This assay is totally efficient and sensitive and could be used as a novel method for visualization of cancer cells.…”

Section: Introductionmentioning

confidence: 99%

Identification and imaging of leukemia cells using dual-aptamer-functionalized graphene oxide complex

et al. 2017

View full text Add to dashboard Cite

Acute lymphoblastic leukemia is the most common malignancy in children. Patient improvement completely depends on the diagnosis of acute lymphoblastic leukemia. So there is a great demand for diagnosis of acute lymphoblastic leukemia. In this study, a novel assay based on dual-aptamer (Sgc8c and ATP aptamers)-functionalized graphene oxide (DAFGO) complex was designed for the identification of Molt-4 cells (human acute lymphoblastic leukemia T-cell). This assay relies on the internalization of DAFGO complex into Molt-4 cells, but not into U266 cells, using Sgc8c aptamer as molecular recognition probe, and release of FAM-labeled ATP aptamer from the complex in the presence of high amounts of ATP in lysosome, leading to a strong fluorescence emission. Formation of DAFGO complex was analyzed by fluorometric analysis and gel retardation assay. The internalization of complex was monitored by flow cytometry and fluorescence microscopy in Molt-4 (target) and U266 cells (nontarget) with DAFGO complex. Our results showed that the developed complex was efficiently internalized into target cells and induced a strong fluorescence emission.

show abstract

Integrating graphene oxide, functional DNA and nucleic-acid-manipulating strategies for amplified biosensing

Cited by 37 publications

References 89 publications

Fluorescent nanoprobes for sensing and imaging of metal ions: Recent advances and future perspectives

Fluorescent nanoprobes for sensing and imaging of metal ions: Recent advances and future perspectives

A Syringe‐Based DNAzyme Sensor for Bacterial Detection

Identification and imaging of leukemia cells using dual-aptamer-functionalized graphene oxide complex

Contact Info

Product

Resources

About