Integrating High-Resolution and Solid-State Magic Angle Spinning NMR Spectroscopy and a Transcriptomic Analysis of Soybean Tissues in Response to Water Deficiency

Coutinho, Isabel; Moraes, Tiago Bueno; Mertz-Henning, L. M.; Nepomuceno, Alexandre Lima; Giordani, Willian; Marcolino-Gomes, Juliana; Santagneli, Silvia H.; Colnago, Luiz Alberto

doi:10.1002/pca.2702

Cited by 11 publications

(24 citation statements)

References 46 publications

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“…To account for the diversity of plant species, their evolving traits during growth, and the development of genetically modified organisms, statistical analysis approaches, more commonly undertaken in metabolomics and 1 H HR-MAS studies of natural products, begin to extend to ssNMR research of lignocellulose. For example, such strategies have been applied to monitor water deficiency effects in order to better understand drought stress on plants . Unfortunately, ssNMR lacks the time efficiency to survey a large number of samplesa mandatory step to match the success of metabolomics in solution NMR. , …”

Section: Solid-state Nmr Studies Of Plant Materialsmentioning

confidence: 99%

“…For example, such strategies have been applied to monitor water deficiency effects in order to better understand drought stress on plants. 332 Unfortunately, ssNMR lacks the time efficiency to survey a large number of samplesa mandatory step to match the success of metabolomics in solution NMR. 333,334 Since NMR resolves differences in chemical environments, changes that occur on biomaterials after they are subjected to any form of exterior physical or chemical perturbation can be easily detected using well-established chemical shifts, 335−337 with the assistance of an online chemical shift database.…”

Section: Polymer Contacts In Secondary Cell Wallsmentioning

confidence: 99%

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Solid-State NMR Investigations of Extracellular Matrixes and Cell Walls of Algae, Bacteria, Fungi, and Plants

et al. 2021

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Extracellular matrixes (ECMs), such as the cell walls and biofilms, are important for supporting cell integrity and function and regulating intercellular communication. These biomaterials are also of significant interest to the production of biofuels and the development of antimicrobial treatment. Solid-state nuclear magnetic resonance (ssNMR) and magic-angle spinning-dynamic nuclear polarization (MAS-DNP) are uniquely powerful for understanding the conformational structure, dynamical characteristics, and supramolecular assemblies of carbohydrates and other biomolecules in ECMs. This review highlights the recent high-resolution investigations of intact ECMs and native cells in many organisms spanning across plants, bacteria, fungi, and algae. We spotlight the structural principles identified in ECMs, discuss the current technical limitation and underexplored biochemical topics, and point out the promising opportunities enabled by the recent advances of the rapidly evolving ssNMR technology.

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Section: Solid-state Nmr Studies Of Plant Materialsmentioning

confidence: 99%

Section: Polymer Contacts In Secondary Cell Wallsmentioning

confidence: 99%

Solid-State NMR Investigations of Extracellular Matrixes and Cell Walls of Algae, Bacteria, Fungi, and Plants

et al. 2021

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“…An increasing number of studies are now using HRMAS NMR to better understand human daily food consumption. Published reports in this arena have included such topics as onion, 127 almonds, 128 grapes, 129 apples, 130 soybean, 131 and smart crops. 132,133 Of note, the results obtained from such studies now appear to be influencing the operations of certain food industries.…”

Section: Metabolomics Studies Of Plants and Foodmentioning

confidence: 99%

High‐resolution magic angle spinning NMR for intact biological specimen analysis: Initial discovery, recent developments, and future directions

Cheng

2022

NMR in Biomedicine

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High‐resolution magic angle spinning (HRMAS) NMR, an approach for intact biological material analysis discovered more than 25 years ago, has been advanced by many technical developments and applied to many biomedical uses. This article provides a history of its discovery, first by explaining the key scientific advances that paved the way for HRMAS NMR's invention, and then by turning to recent developments that have profited from applying and advancing the technique during the last 5 years. Developments aimed at directly impacting healthcare include HRMAS NMR metabolomics applications within studies of human disease states such as cancers, brain diseases, metabolic diseases, transplantation medicine, and adiposity. Here, the discussion describes recent HRMAS NMR metabolomics studies of breast cancer and prostate cancer, as well as of matching tissues with biofluids, multimodality studies, and mechanistic investigations, all conducted to better understand disease metabolic characteristics for diagnosis, opportune windows for treatment, and prognostication. In addition, HRMAS NMR metabolomics studies of plants, foods, and cell structures, along with longitudinal cell studies, are reviewed and discussed. Finally, inspired by the technique's history of discoveries and recent successes, future biomedical arenas that stand to benefit from HRMAS NMR‐initiated scientific investigations are presented.

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“…For instance, the study of soybean leaves grown with and without water-deficiency stress with HRMAS, revealed differences in the metabolite profiles. A total of 30 metabolites were identified, with the amino acid metabolites present in the metabolite profile of soybean leaves with water-deficient stress [ 129 ]. HRMAS can therefore aid in elucidating plant response mechanisms.…”

Section: 4ir Technologies and Plant Metabolomicsmentioning

confidence: 99%

Metabolomics-Guided Elucidation of Plant Abiotic Stress Responses in the 4IR Era: An Overview

et al. 2021

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Plants are constantly challenged by changing environmental conditions that include abiotic stresses. These are limiting their development and productivity and are subsequently threatening our food security, especially when considering the pressure of the increasing global population. Thus, there is an urgent need for the next generation of crops with high productivity and resilience to climate change. The dawn of a new era characterized by the emergence of fourth industrial revolution (4IR) technologies has redefined the ideological boundaries of research and applications in plant sciences. Recent technological advances and machine learning (ML)-based computational tools and omics data analysis approaches are allowing scientists to derive comprehensive metabolic descriptions and models for the target plant species under specific conditions. Such accurate metabolic descriptions are imperatively essential for devising a roadmap for the next generation of crops that are resilient to environmental deterioration. By synthesizing the recent literature and collating data on metabolomics studies on plant responses to abiotic stresses, in the context of the 4IR era, we point out the opportunities and challenges offered by omics science, analytical intelligence, computational tools and big data analytics. Specifically, we highlight technological advancements in (plant) metabolomics workflows and the use of machine learning and computational tools to decipher the dynamics in the chemical space that define plant responses to abiotic stress conditions.

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Integrating High-Resolution and Solid-State Magic Angle Spinning NMR Spectroscopy and a Transcriptomic Analysis of Soybean Tissues in Response to Water Deficiency

Abstract: The integration of the H HR-MAS and SSNMR spectra with the transcriptomic data provided a complete picture of the major changes in the metabolic profile of soybeans in response to water deficiency. Copyright © 2017 John Wiley& Sons, Ltd.

Cited by 11 publications

References 46 publications

Solid-State NMR Investigations of Extracellular Matrixes and Cell Walls of Algae, Bacteria, Fungi, and Plants

Solid-State NMR Investigations of Extracellular Matrixes and Cell Walls of Algae, Bacteria, Fungi, and Plants

High‐resolution magic angle spinning NMR for intact biological specimen analysis: Initial discovery, recent developments, and future directions

Metabolomics-Guided Elucidation of Plant Abiotic Stress Responses in the 4IR Era: An Overview

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