Integrating insulin into single-step culture medium regulates human embryo development in vitro

Fawzy, Mohamed; Sabry, Mohamed; Nour, Mohamed; Abdel-Rahman, Mohamed; Roshdy, Eman; Magdi, Yasmin; Abdelghafar, Hazem

doi:10.1016/j.fertnstert.2016.11.003

Cited by 5 publications

(3 citation statements)

References 37 publications

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“…The Gardner system has resulted in a plethora of studies using the threshold 3BB as the minimal grade for blastocyst usability (Arce et al , 2014; Rubio et al , 2014; Comstock et al , 2015; Munch et al , 2015). In studies using the newer simplified grading systems, similarly grade 3/C blastocysts are often not frozen or biopsied (Restelli et al , 2014; Stone et al , 2014; Hardarson et al , 2015; Richardson et al , 2015; Fawzy et al , 2017). Since low quality blastocysts can lead to live births (Capalbo et al , 2014; Wirleitner et al , 2016; Irani et al , 2017) with normal obstetric and perinatal outcomes (Bouillon et al , 2017), this bias against freezing grade ‘C’ blastocysts has limited the establishment of a lower threshold for viability.…”

Section: Blastocyst Grades and Viabilitymentioning

confidence: 99%

Blastocyst culture in the Era of PGS and FreezeAlls: Is a ‘C’ a failing grade?

Morbeck

2017

Human Reproduction Open

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Section: Blastocyst Grades and Viabilitymentioning

confidence: 99%

Blastocyst culture in the Era of PGS and FreezeAlls: Is a ‘C’ a failing grade?

Morbeck

2017

Human Reproduction Open

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“…The concept is to place different types of cell [ 69 ] or tissue cultures within environments (culture medium) that mimic the in vivo obesity environments in terms of pH [ 70 , 71 ], inflammatory factors, insulin [ 72 , 73 ], hormonal factors [ 74 , 75 ], lipids content, glucose, etc. ( Figure 1 ).…”

Section: In Vitro Obesity-related Environmentmentioning

confidence: 99%

In Vitro Mimicking of Obesity-Induced Biochemical Environment to Study Obesity Impacts on Cells and Tissues

2022

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Obesity represents a heavy burden for modern healthcare. The main challenge facing obesity research progress is the unknown underlying pathways, which limits our understanding of the pathogenesis and developing therapies. Obesity induces specific biochemical environments that impact the different cells and tissues. In this piece of writing, we suggest mimicking obesity-induced in vivo biochemical environments including pH, lipids, hormones, cytokines, and glucose within an in vitro environment. The concept is to reproduce such biochemical environments and use them to treat the tissue cultures, explant cultures, and cell cultures of different biological organs. This will allow us to clarify how the obesity-induced biochemistry impacts such biological entities. It would also be important to try different environments, in terms of the compositions and concentrations of the constitutive elements, in order to establish links between the effects (impaired regeneration, cellular inflammation, etc.) and the factors constituting the environment (hormones, cytokines, etc.) as well as to reveal dose-dependent effects. We believe that such approaches will allow us to elucidate obesity mechanisms, optimize animal models, and develop therapies as well as novel tissue engineering applications.

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“…Allegation: "The first RCT (5) was registered late." This allegation refers to our comparative study, "Integrating insulin into single-step culture medium regulates human embryo development in vitro (5)." Rebuttal: We did not report this study as a randomised, controlled trial (RCT).…”

Section: Refutationmentioning

confidence: 99%

False allegations cannot go unchallenged: an appeal to shield researchers from dubious methods and ambiguous motives

Fawzy¹

2022

Preprint

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Before launching an investigation or releasing allegations, researchers, journals, and the public should seek advice from statisticians and epidemiologists who do not work directly or indirectly with complainants or engage in whistleblowing. Journals should refrain from publishing defamatory statements based on unproven methods. In line with this concept, JAMA published an editorial (1) stating that “the first step involves determining the validity of the allegation and an assessment of whether the allegation is consistent with the definition of research misconduct... In some cases, when authors are accused of misconduct, the criticism represents a different interpretation of the data or disagreement with the statistical approach used, rather than scientific misconduct...This initial step also involves determining whether the individuals alleging misconduct have relevant conflicts of interest.” In this scientific rebuttal, I provide a real-world example of how unproven methods can damage and stress out researchers, how they should respond, and when they should disclose the case to the public.

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Integrating insulin into single-step culture medium regulates human embryo development in vitro

Cited by 5 publications

References 37 publications

Blastocyst culture in the Era of PGS and FreezeAlls: Is a ‘C’ a failing grade?

Blastocyst culture in the Era of PGS and FreezeAlls: Is a ‘C’ a failing grade?

In Vitro Mimicking of Obesity-Induced Biochemical Environment to Study Obesity Impacts on Cells and Tissues

False allegations cannot go unchallenged: an appeal to shield researchers from dubious methods and ambiguous motives

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