Integrating metabolomics and network pharmacology to explore Rhizoma Coptidis extracts against sepsis-associated acute kidney injury

Zheng, Yimei; Shi, Xuan; Hou, Jiebin; Gao, Songyan; Chao, Yufan; Ding, Jiarong; Chen, Long; Qian, Yongfang; Shao, Guojian; Si, Yanna; Chen, Wei

doi:10.1016/j.jchromb.2021.122525

Cited by 17 publications

(12 citation statements)

References 44 publications

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“…PPARα has been identified as a direct target of miR-372-3p in HK-2 cells [21,22]. Zhong et al reported that formononetin treats acute kidney injury through activation of thePPARα/Nrf2/HO-1 pathway [23].…”

Section: Data Availabilitymentioning

confidence: 99%

Long Noncoding RNA ZFAS1 Protects HK-2 Cells against Sepsis‐Induced Injury through Targeting the miR3723p/PPARα Axis

Hei

Yue

Sun

2022

Journal of Healthcare Engineering

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In septic acute kidney injury, one of the main purposes of long noncoding RNA (lncRNA) ZFAS1 is still unclear. This study is intended to analyze the effects of lncRNA ZFAS1 on the septic AKI in the HK-2 cell line. Materials and Methods. In order to construct an in vitro model of septic AKI, HK-2 cells have been treated with lipopolysaccharides. CCK-8 assay has been utilized to check the viability of HK-2 cells. The contents of inflammatory cytokines (that includes IL-1β, TNF-α, and IL-6) have been marked with enzyme-linked immune sorbent assay (ELISA). Cell apoptosis was assessed by TUNEL staining. To detect the expression of lncRNA ZFAS1 and microRNA-372-3p, quantitative reverse-transcription PCR has been used. And to confirm the connection among genes, luciferase reporter assay has been applied. Results. Overexpression of ZFAS1 alleviated LPS‐induced HK-2 cell injury. ZFAS1 positively regulated expression of α receptor activated by peroxisome proliferation (PPARα) through competitive linkage with miR-372-3p. In addition, over expression of miR-372-3p counteracted the protective effect of upward regulation of ZFAS1 on LPS-induced HK-2 cell damage, which could be reversed by over expression of PPARα. Conclusion. It is concluded that, in LPS-induced HK-2 cell injury, ZFAS1 has a protective role via modulating the miR-372-3p/PPARα axis, suggesting the potential of ZFAS1 as a protective target for septic AKI.

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Section: Data Availabilitymentioning

confidence: 99%

Long Noncoding RNA ZFAS1 Protects HK-2 Cells against Sepsis‐Induced Injury through Targeting the miR3723p/PPARα Axis

Hei

Yue

Sun

2022

Journal of Healthcare Engineering

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“… Regulating metabolism via different signaling pathways. [ 102 ] Brain Mice; CLP Berberine alleviated cognitive impairment. Blocking HMGB1/RAGE Signaling.…”

Section: Introductionmentioning

confidence: 99%

Anti-Hyperglycemic Agents in the Adjuvant Treatment of Sepsis: Improving Intestinal Barrier Function

Wang¹,

Li²,

Wang³

et al. 2022

DDDT

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Intestinal barrier injury and hyperglycemia are common in patients with sepsis. Bacteria translocation and systemic inflammatory response caused by intestinal barrier injury play a significant role in sepsis occurrence and deterioration, while hyperglycemia is linked to adverse outcomes in sepsis. Previous studies have shown that hyperglycemia is an independent risk factor for intestinal barrier injury. Concurrently, increasing evidence has indicated that some anti-hyperglycemic agents not only improve intestinal barrier function but are also beneficial in managing sepsis-induced organ dysfunction. Therefore, we assume that these agents can block or reduce the severity of sepsis by improving intestinal barrier function. Accordingly, we explicated the connection between sepsis, intestinal barrier, and hyperglycemia, overviewed the evidence on improving intestinal barrier function and alleviating sepsis-induced organ dysfunction by anti-hyperglycemic agents (eg, metformin, peroxisome proliferators activated receptor-γ agonists, berberine, and curcumin), and summarized some common characteristics of these agents to provide a new perspective in the adjuvant treatment of sepsis.

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“…Cordyceps sinensis has a protective effect on LPS induced intestinal injury, which can promote cell proliferation and restore intestinal barrier function (Gu et al, 2015). Rhizoma Coptidis protects against sepsis related AKI by acting on HO-1, NOS2 and PPAR α and other 17 target proteins (Zheng et al, 2021). Lonicera japonica Thunb.…”

Section: Single Herbal Medicinesmentioning

confidence: 99%

Traditional Chinese Medicine: A promising strategy to regulate inflammation, intestinal disorders and impaired immune function due to sepsis

Wang

Chen

et al. 2022

Front. Pharmacol.

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Sepsis is described as a dysregulation of the immune response to infection, which leads to life-threatening organ dysfunction. The interaction between intestinal microbiota and sepsis can’t be ignored. Furthermore, the intestinal microbiota may regulate the progress of sepsis and attenuate organ damage. Thus, maintaining or restoring microbiota may be a new way to treat sepsis. Traditional Chinese medicine (TCM) assumes a significant part in the treatment of sepsis through multi-component, multi-pathway, and multi-targeting abilities. Moreover, TCM can prevent the progress of sepsis and improve the prognosis of patients with sepsis by improving the imbalance of intestinal microbiota, improving immunity and reducing the damage to the intestinal barrier. This paper expounds the interaction between intestinal microbiota and sepsis, then reviews the current research on the treatment of sepsis with TCM, to provide a theoretical basis for its clinical application.

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Integrating metabolomics and network pharmacology to explore Rhizoma Coptidis extracts against sepsis-associated acute kidney injury

Cited by 17 publications

References 44 publications

Long Noncoding RNA ZFAS1 Protects HK-2 Cells against Sepsis‐Induced Injury through Targeting the miR3723p/PPARα Axis

Long Noncoding RNA ZFAS1 Protects HK-2 Cells against Sepsis‐Induced Injury through Targeting the miR3723p/PPARα Axis

Anti-Hyperglycemic Agents in the Adjuvant Treatment of Sepsis: Improving Intestinal Barrier Function

Traditional Chinese Medicine: A promising strategy to regulate inflammation, intestinal disorders and impaired immune function due to sepsis

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