Integrating serum microRNAs and electronic health records improved the diagnosis of tuberculosis

Gao, Shanxing; Chen, Chunguang; Zhuang, Chunbo; Zeng, Yu-Ling; Zeng, Zhenzhen; Yong-min, YU; Ming, Liang; Zhao, Junwei

doi:10.1002/jcla.23871

Cited by 5 publications

(4 citation statements)

References 41 publications

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“…Qi et al, reported that the combination of miR-361-5p, miR-889, and miR-576-3p displayed strong potential for diagnostic utility with a high AUC value of 0.863 [ 51 ]. Additionally, previous studies have suggested that miR-30c and miR-142-3p serve as diagnostic biomarkers, with AUC values of 0.67 and 0.75, respectively [ 52 ]. Jia-Yi Cui et al, reported that AUC values of miR-769-5p, miR-320a, and miR-22-3p range from 0.69 to 0.97 in qRT-PCR validation [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of MicroRNAs as Potential Blood-Based Biomarkers for Diagnosis and Therapeutic Monitoring of Active Tuberculosis

Kim

Park

et al. 2022

Diagnostics

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Early diagnosis increases the treatment success rate for active tuberculosis (ATB) and decreases mortality. MicroRNAs (miRNAs) have been studied as blood-based markers of several infectious diseases. We performed miRNA profiling to identify differentially expressed (DE) miRNAs using whole blood samples from 10 healthy controls (HCs), 15 subjects with latent tuberculosis infection (LTBI), and 12 patients with ATB, and investigated the expression of the top six miRNAs at diagnosis and over the treatment period in addition to performing miRNA-target gene network and gene ontology analyses. miRNA profiling identified 84 DE miRNAs in patients with ATB, including 80 upregulated and four downregulated miRNAs. Receiver operating characteristic curves of the top six miRNAs exhibited excellent distinguishing efficiency with an area under curve (AUC) value > 0.85. Among them, miR-199a-3p and miR-6886-3p can differentiate between ATB and LTBI. Anti-TB treatment restored the levels of miR-199b-3p, miR-199a-3p, miR-16-5p, and miR-374c-5p to HC levels. Furthermore, 108 predicted target genes were related to the regulation of cellular amide metabolism, intrinsic apoptotic signaling, translation, transforming growth factor beta receptor signaling, and cysteine-type endopeptidase activity. The DE miRNAs identified herein are potential biomarkers for diagnosis and therapeutic monitoring in ATB.

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Section: Discussionmentioning

confidence: 99%

Identification of MicroRNAs as Potential Blood-Based Biomarkers for Diagnosis and Therapeutic Monitoring of Active Tuberculosis

Kim

Park

et al. 2022

Diagnostics

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“…These circulating plasma miRNAs can further enable the differential diagnosis of latent and active TB. Diagnostic performance of miRNA can be increased by integrating serum miRNAs with diagnostic models developed by miRNA characteristics and electronic health records (HERs) [61,62] and bioinformatics analysis [63]. Even though miRNA-based detection of TB is convenient in diagnosis in children, the method is challenging to adopt in resource-limited settings.…”

Section: Microrna Detectionmentioning

confidence: 99%

Tuberculosis Diagnosis: Updates and Challenges

Shivakumar¹,

Shettigar²

2023

Bacterial Infectious Diseases Annual Volume 2023

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Tuberculosis (TB) is caused by a single infectious agent, Mycobacterium tuberculosis, and a public health concern due to increased cases of drug-resistance and high mortality rates. Rapid identification of tuberculosis is necessary for its early treatment and to prevent the emergence of drug-resistant strains. For effective management of patients, rapid, cost-effective, and point-of-care (POC) diagnostic methods are required. The commonly used screening and identification methods are clinical examination, radiography, sputum smear microscopy, culture method, serological method, and tuberculin skin test. In addition, several molecular methods such as NAAT based GeneXpert, loop-mediated isothermal amplification (LAMP), line probe assay (LPA), whole genome sequencing (WGS) and other non-invasive methods of lateral flow urine lipoarabinomannan assay (LF-LAM) and eNose assays are developed. Sputum smear microscopy, Xpert MTB/RIF, and LED-Fluorescence microscopy (LED-FM) are the preferred methods to use in peripheral laboratories. The non-invasive methods of tuberculosis diagnosis are more beneficial in patients from whom collecting sputum sample is difficult particularly in children and HIV co-infected patients. Molecular methods can simultaneously identify the pathogen, M. tuberculosis, and mutations in drug-resistance genes. Even though, many advanced methods are currently available, accurate and affordable diagnostic method for tuberculosis is still challenging. Here, we review and highlight the uses and challenges of currently available conventional and advanced diagnostic methods of tuberculosis screening and diagnosis.

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“…After obtaining this result, they utilized a combination of proteins and miRNAs to further improve the model ( 185 ). Another group combined miR-142-3p with electronic health record (EHR) data to detect TB, and the AUC reached 0.94 ( 186 ). Compared with LTBI patients, hsa-miR-1246, hsa-miR-2110, hsa-miR-370-3p, hsa-miR-193b-5p, and hsa-miR-28-3p are specifically expressed in TB cohorts ( 187 ).…”

Section: Noncoding Rnas As Biomarkers In Tuberculosismentioning

confidence: 99%

Immune regulation and emerging roles of noncoding RNAs in Mycobacterium tuberculosis infection

Liang

Ma²,

Gong³

et al. 2022

Front. Immunol.

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Tuberculosis, caused by Mycobacterium tuberculosis, engenders an onerous burden on public hygiene. Congenital and adaptive immunity in the human body act as robust defenses against the pathogens. However, in coevolution with humans, this microbe has gained multiple lines of mechanisms to circumvent the immune response to sustain its intracellular persistence and long-term survival inside a host. Moreover, emerging evidence has revealed that this stealthy bacterium can alter the expression of demic noncoding RNAs (ncRNAs), leading to dysregulated biological processes subsequently, which may be the rationale behind the pathogenesis of tuberculosis. Meanwhile, the differential accumulation in clinical samples endows them with the capacity to be indicators in the time of tuberculosis suffering. In this article, we reviewed the nearest insights into the impact of ncRNAs during Mycobacterium tuberculosis infection as realized via immune response modulation and their potential as biomarkers for the diagnosis, drug resistance identification, treatment evaluation, and adverse drug reaction prediction of tuberculosis, aiming to inspire novel and precise therapy development to combat this pathogen in the future.

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Integrating serum microRNAs and electronic health records improved the diagnosis of tuberculosis

Cited by 5 publications

References 41 publications

Identification of MicroRNAs as Potential Blood-Based Biomarkers for Diagnosis and Therapeutic Monitoring of Active Tuberculosis

Identification of MicroRNAs as Potential Blood-Based Biomarkers for Diagnosis and Therapeutic Monitoring of Active Tuberculosis

Tuberculosis Diagnosis: Updates and Challenges

Immune regulation and emerging roles of noncoding RNAs in Mycobacterium tuberculosis infection

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