Integrating the MasSpec Pen to the da Vinci Surgical System for <i>In Vivo</i> Tissue Analysis during a Robotic Assisted Porcine Surgery

Keating, Michael F.; Zhang, Jialing; Feider, Clara L.; Retailleau, Sascha; Reid, R. Clay; Antaris, Alexander L.; Hart, Bradley R.; Tan, Gina; Milner, Thomas E.; Miller, Kyle R.; Eberlin, Lívia S.

doi:10.1021/acs.analchem.0c02037

Cited by 59 publications

(45 citation statements)

References 32 publications

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“…We also foresee development of the devices for surgical interventions as add-ons in robotic-assisted tools such as the Da Vinci Robot (Intuitive Surgical) [84] or RoSA (Zimmer Biomet) [85] and combined with AI screening tools for minimally invasive and guided surgery. Some efforts have already been made in that direction with the development of the endoscopic REIMS system [32] and the coupling of the MasSpec Pen with the Da Vinci Robot on a porcine model simulation [86].…”

Section: Discussionmentioning

confidence: 99%

Cancer Surgery 2.0: Guidance by Real-Time Molecular Technologies

Ogrinc

Saudemont

Takáts

et al. 2021

Trends in Molecular Medicine

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In vivo cancer margin delineation during surgery remains a major challenge. Despite the availability of several image guidance techniques and intraoperative assessment, clear surgical margins and debulking efficiency remain scarce. For this reason, there is particular interest in developing rapid intraoperative tools with high sensitivity and specificity to help guide cancer surgery in vivo. Recently, several emerging technologies including intraoperative mass spectrometry have paved the way for molecular guidance in a clinical setting. We evaluate these techniques and assess their relevance for intraoperative surgical guidance and how they can transform the future of molecular cancer surgery, diagnostics, patient management and care. HighlightsCancer surgery is the first treatment in many solid tumors. Quality of the initial surgery has a strong influence on the patient outcome. Tumors must be excised at best, but accurate delineation of their edges and margin definition is a huge challenge.

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Section: Discussionmentioning

confidence: 99%

Cancer Surgery 2.0: Guidance by Real-Time Molecular Technologies

Ogrinc

Saudemont

Takáts

et al. 2021

Trends in Molecular Medicine

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“…Furthermore, MasSpec Pen has ever been introduced into the porcine upper GI tracts in a study. The accuracy of distinguishing the liver from the stomach tissues in vivo was 98%[ 60 ]. In fact, utilizing MasSpec Pen for any cancer diagnosis was solely dependent on the availability of the corresponding tissue-specific database[ 59 ].…”

Section: Pathological Diagnosis Of Gi Cancermentioning

confidence: 99%

Application of metabolomics in clinical and laboratory gastrointestinal oncology

Gao¹,

Huang²,

Fang³

et al. 2021

WJGO

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Metabolites are versatile bioactive molecules. They are not only the substrates and/or the products of enzymatic reactions but also act as the regulators in the systemic metabolism. Metabolomics is a high-throughput analytical strategy to qualify or quantify as many metabolites as possible in the metabolomes. It is an indispensable part of systems biology. The leading techniques in this field are mainly based on mass spectrometry and nuclear magnetic resonance spectroscopy. The metabolomic analysis has gained wide use in bioscience fields. In the tumor research arena, metabolomics can be employed to identify biomarkers for prediction, diagnosis, and prognosis. Chemotherapeutic effect evaluation and personalized medicine decision-making can also benefit from metabolomic analysis of patient biofluid or biopsy samples. Many cell-level studies can help in disease exploration. In this review, the basic features and principles of varied metabolomic analysis are introduced. The value of metabolomics in clinical and laboratory gastrointestinal cancer studies is discussed, especially for mass spectrometry applications. Besides, combined use of metabolomics and other tools to solve problems in cancer practice is briefly illustrated. In summary, metabolomics paves a new way to explore cancerous diseases in the light of small molecules.

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“…Further, spatial proteomics specifically in the form of mass spectrometry imaging proteomics is an emerging technology inside the surgical theater. For example, the mass spectrometry 'pen' system is a research-grade medical device that aids surgeons in excising all cancer tissue surrounding a tumor by detecting any cancer proteins remaining in the surgical margins [78][79][80].…”

Section: Opportunities and Future Directionsmentioning

confidence: 99%

Proximity labeling and other novel mass spectrometric approaches for spatiotemporal protein dynamics

Pino

Schilling

2021

Expert Review of Proteomics

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Background: Proteins are highly dynamic and their biological function is controlled by not only temporal abundance changes but also via regulated protein-protein interaction networks, which respond to internal and external perturbations. A wealth of novel analytical reagents and workflows allow studying spatiotemporal protein environments with great granularity while maintaining high throughput and ease of analysis. Areas covered: We review technology advances for measuring protein-protein proximity interactions with an emphasis on proximity labeling, and briefly summarize other spatiotemporal approaches including protein localization, and their dynamic changes over time, specifically in human cells and mammalian tissues. We focus especially on novel technologies and workflows emerging within the past 5 years. This includes enrichment-based techniques (proximity labeling and crosslinking), separationbased techniques (organelle fractionation and size exclusion chromatography), and finally sortingbased techniques (laser capture microdissection and mass spectrometry imaging). Expert opinion: Spatiotemporal proteomics is a key step in assessing biological complexity, understanding refined regulatory mechanisms, and forming protein complexes and networks. Studying protein dynamics across space and time holds promise for gaining deep insights into how protein networks may be perturbed during disease and aging processes, and offer potential avenues for therapeutic interventions, drug discovery, and biomarker development.

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Integrating the MasSpec Pen to the da Vinci Surgical System for In Vivo Tissue Analysis during a Robotic Assisted Porcine Surgery

Cited by 59 publications

References 32 publications

Cancer Surgery 2.0: Guidance by Real-Time Molecular Technologies

Cancer Surgery 2.0: Guidance by Real-Time Molecular Technologies

Application of metabolomics in clinical and laboratory gastrointestinal oncology

Proximity labeling and other novel mass spectrometric approaches for spatiotemporal protein dynamics

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