Integrating Waveguide Biosensor

S, Li; Amstutz, P; Cm, Tang; J, Hang; Zhu, Ping; Zhang, Y; Dr, Shelton; Js, Karns

doi:10.1007/978-1-60327-567-5_22

Cited by 7 publications

(5 citation statements)

References 7 publications

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“…The various modes of transduction that are fluorescence, interferometry, radiolabeling, and others, used in conjunction with waveguides and fiber optics for biodetection, have been extensively reviewed earlier [ 54 ]. This review will discuss some of the fluorescent and interferometry-based detection platforms used in conjunction with planar optical waveguides for efficient biodetection.…”

Section: Modes Of Transductionmentioning

confidence: 99%

Waveguide-Based Biosensors for Pathogen Detection

Mukundan

Anderson

Grace

et al. 2009

Sensors

172

128

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Optical phenomena such as fluorescence, phosphorescence, polarization, interference and non-linearity have been extensively used for biosensing applications. Optical waveguides (both planar and fiber-optic) are comprised of a material with high permittivity/high refractive index surrounded on all sides by materials with lower refractive indices, such as a substrate and the media to be sensed. This arrangement allows coupled light to propagate through the high refractive index waveguide by total internal reflection and generates an electromagnetic wave—the evanescent field—whose amplitude decreases exponentially as the distance from the surface increases. Excitation of fluorophores within the evanescent wave allows for sensitive detection while minimizing background fluorescence from complex, “dirty” biological samples. In this review, we will describe the basic principles, advantages and disadvantages of planar optical waveguide-based biodetection technologies. This discussion will include already commercialized technologies (e.g., Corning’s EPIC® Ô, SRU Biosystems’ BIND™, Zeptosense®, etc.) and new technologies that are under research and development. We will also review differing assay approaches for the detection of various biomolecules, as well as the thin-film coatings that are often required for waveguide functionalization and effective detection. Finally, we will discuss reverse-symmetry waveguides, resonant waveguide grating sensors and metal-clad leaky waveguides as alternative signal transducers in optical biosensing.

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Section: Modes Of Transductionmentioning

confidence: 99%

Waveguide-Based Biosensors for Pathogen Detection

Mukundan

Anderson

Grace

et al. 2009

Sensors

172

128

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“…The results showed that the new approach was feasible due to the use of an ultra-sensitive Signalyte™-II fluorescent instrument platform [31–33], allowing for a small number of bead-captured bacteria (LOD 10 CFU) to be directly detected by a fluorescent detector antibody without the need of a pre-enrichment step. In this study, we demonstrated that the same approach could be used for detection of ZAP-70 protein in CLL with a modified assay protocol.…”

Section: Discussionmentioning

confidence: 99%

Quantitative detection of zeta-chain-associated protein 70 expression in chronic lymphocytic leukemia

Zhu

Degheidy

Marti

et al. 2012

Leukemia & Lymphoma

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Overexpression of zeta-chain-associated protein 70 (ZAP-70) was recently recognized as an independent prognostic marker for the aggressive form of chronic lymphocytic leukemia (CLL). The objective of this study was to demonstrate the feasibility and implementation of quantitative detection of ZAP-70 protein in B cells to clearly distinguish patients with CLL with the aggressive form of the disease. B cells were isolated from patient blood and lysed. Released ZAP-70 protein was detected using an immunomagnetic fluorescence assay. The assay protocol was developed using Jurkat cells and recombinant ZAP-70 (rZAP-70). The limit of detection was determined to be lower than 125 Jurkat cells and 39 pg of rZAP-70 protein. The signal response was linear over a wide dynamic range, from 125 to 40 000 Jurkat cells per test (R2 = 0.9987) and from 0 to 40 000 pg rZAP-70 protein per test (R2 = 0.9928). The results from 20 patients with CLL correlated strongly with flow cytometry analysis. Concordance between the two methods for positive and negative results was 100% (7/7) and 92% (12/13), respectively, while the overall concordance between the two methods was 95%. The assay reported here is a simple, reliable and reproducible method for quantitative detection of ZAP-70 in patient leukemic cells, without the need for cell fixation or permeabilization. The ZAP-70 signal was linear over a wide dynamic range, which we believe enables quantitative assessment of small changes in ZAP-70 expression over the course of the disease and in response to therapeutic intervention.

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“…This category deals with articles on optical fiber sensor methods and applications to detect the COVID-19 virus. A significant number of optical biosensors are based mainly on the surface plasmonic resonance concept [118] including when optical components such as waveguides are used in modulation principles [119], based on a photon crystal fiber (PCF) [24], fiber optics based on wavelength [120] and by using nanolaser [23] and are categorized into optical sensors. Typically, the optical sensor consists of a light, detector and optoelectronic transducer.…”

Section: Optical Biosensorsmentioning

confidence: 99%

An Analysis Review of Detection Coronavirus Disease 2019 (COVID-19) Based on Biosensor Application

Taha

Mashhadany

Mokhtar

et al. 2020

Sensors

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Timely detection and diagnosis are essentially needed to guide outbreak measures and infection control. It is vital to improve healthcare quality in public places, markets, schools and airports and provide useful insights into the technological environment and help researchers acknowledge the choices and gaps available in this field. In this narrative review, the detection of coronavirus disease 2019 (COVID-19) technologies is summarized and discussed with a comparison between them from several aspects to arrive at an accurate decision on the feasibility of applying the best of these techniques in the biosensors that operate using laser detection technology. The collection of data in this analysis was done by using six reliable academic databases, namely, Science Direct, IEEE Xplore, Scopus, Web of Science, Google Scholar and PubMed. This review includes an analysis review of three highlights: evaluating the hazard of pandemic COVID-19 transmission styles and comparing them with Severe Acute Respiratory Syndrome (SARS) and Middle East Respiratory Syndrome (MERS) to identify the main causes of the virus spreading, a critical analysis to diagnose coronavirus disease 2019 (COVID-19) based on artificial intelligence using CT scans and CXR images and types of biosensors. Finally, we select the best methods that can potentially stop the propagation of the coronavirus pandemic.

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Integrating Waveguide Biosensor

Cited by 7 publications

References 7 publications

Waveguide-Based Biosensors for Pathogen Detection

Waveguide-Based Biosensors for Pathogen Detection

Quantitative detection of zeta-chain-associated protein 70 expression in chronic lymphocytic leukemia

An Analysis Review of Detection Coronavirus Disease 2019 (COVID-19) Based on Biosensor Application

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