Integration of a Miniature Quartz Crystal Microbalance with a Microfluidic Chip for Amyloid Beta-Aβ42 Quantitation

Tao, Wenyan; Xie, Qingji; Wang, Hairui; Ke, Shanming; Lin, Peng; Zeng, Xierong

doi:10.3390/s151025746

Cited by 19 publications

(10 citation statements)

References 32 publications

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“…QCM-D is capable of characterizing interfacial structure, binding kinetics, molecular affinity, and mechanical properties of the adsorbent . Advantages of the acoustic technique include label-free, real-time measurements and ease of miniaturization . For a 5-MHz resonator in water, the acoustic wave possesses a penetration depth of around 250 nm from the oscillator surface, which matches the size of the exosomes .…”

Section: Introductionmentioning

confidence: 99%

Acoustic Immunosensing of Exosomes Using a Quartz Crystal Microbalance with Dissipation Monitoring

et al. 2020

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Exosomes are endocytic lipid-membrane bound bodies with the potential to be used as biomarkers in cancer and neurodegenerative disease. The limitations and scarcity of current exosome characterization approaches have led to a growing demand for translational techniques, capable of determining their molecular composition and physical properties in physiological fluids. Here, we investigate label-free immunosensing, using a quartz crystal microbalance with dissipation monitoring (QCM-D), to detect exosomes by exploiting their surface protein profile. Exosomes expressing the transmembrane protein CD63 were isolated by size-exclusion chromatography from cell culture media. QCM-D sensors functionalized with anti-CD63 antibodies formed a direct immunoassay toward CD63-positive exosomes in 75% v/v serum, exhibiting a limit-of-detection of 2.9 × 108 and 1.4 × 108 exosome sized particles (ESPs)/mL for frequency and dissipation response, respectively, i.e., clinically relevant concentrations. Our proof-of-concept findings support the adoption of dual-mode acoustic analysis of exosomes, leveraging both frequency and dissipation monitoring for use in bioanalytical characterization.

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Section: Introductionmentioning

confidence: 99%

Acoustic Immunosensing of Exosomes Using a Quartz Crystal Microbalance with Dissipation Monitoring

et al. 2020

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“…Microfluidic exosome capture and lysing techniques featured in Ko 2017 and Ramshani 2019 could be utilized for isolation of neural exosomes from blood and freeing of their neuro biomarker cargo. Furthermore, once freely soluble, these neuro biomarkers can be detected for their clinical relevance and AD or PD diagnosis in a similar fashion to the previously described methods [41][42][43]47,[49][50][51]. Any combination of these techniques can be applied to enhance microfluidic neurodegenerative exosome research and offer a substantial effort in furthering more facile, real time, early detection of neurodegenerative disorders.…”

Section: Opportunities To Develop Technologies For Profiling Of Exosomal Cargo Biomarkersmentioning

confidence: 99%

“…This technique is favorable because it presents a label-free strategy for detecting aging biomarkers. The principles behind MQCM are that once the mass of a sample is loading on the piezoelectric active crystal area, there is a shift in the resonance frequency, which is directly detected by a gold-plated sensor [42]. Specific detection of Aβ42 was achieved with a cross-linker and Aβ42 antibody immobilization on the piezoelectric detecting reservoir.…”

Section: Microfluidic Detection Of Alzheimer's Disease Biomarkers: Tau Protein and Amyloid-betamentioning

confidence: 99%

The Microfluidic Toolbox for Analyzing Exosome Biomarkers of Aging

et al. 2021

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As the fields of aging and neurological disease expand to liquid biopsies, there is a need to identify informative biomarkers for the diagnosis of neurodegeneration and other age-related disorders such as cancers. A means of high-throughput screening of biomolecules relevant to aging can facilitate this discovery in complex biofluids, such as blood. Exosomes, the smallest of extracellular vesicles, are found in many biofluids and, in recent years, have been found to be excellent candidates as liquid biopsy biomarkers due to their participation in intercellular communication and various pathologies such as cancer metastasis. Recently, exosomes have emerged as novel biomarkers for age-related diseases. Hence, the study of exosomes, their protein and genetic cargo can serve as early biomarkers for age-associated pathologies, especially neurodegenerative diseases. However, a disadvantage of exosome studies includes a lack in standardization of isolating, detecting, and profiling exosomes for downstream analysis. In this review, we will address current techniques for high-throughput isolation and detection of exosomes through various microfluidic and biosensing strategies and how they may be adapted for the detection of biomarkers of age-associated disorders.

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“…In recent years, QCM systems have been miniaturised and integrated into microfluidic chips to perform detection assays ( Fig. 8 b) ( Tao et al, 2015 ; Thies et al, 2017 ). The ability of QCM to be operated under continuous or partial flow modes makes it compatible for integration with microfluidic systems.…”

Section: Research Opportunitiesmentioning

confidence: 99%

Quartz crystal microbalance-based biosensors as rapid diagnostic devices for infectious diseases

Lim

Saha

Tey

et al. 2020

Biosensors and Bioelectronics

159

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Infectious diseases are the ever-present threats to public health and the global economy. Accurate and timely diagnosis is crucial to impede the progression of a disease and break the chain of transmission. Conventional diagnostic techniques are typically time-consuming and costly, making them inefficient for early diagnosis of infections and inconvenient for use at the point of care. Developments of sensitive, rapid, and affordable diagnostic methods are necessary to improve the clinical management of infectious diseases. Quartz crystal microbalance (QCM) systems have emerged as a robust biosensing platform due to their label-free mechanism, which allows the detection and quantification of a wide range of biomolecules. The high sensitivity and short detection time offered by QCM-based biosensors are attractive for the early detection of infections and the routine monitoring of disease progression. Herein, the strategies employed in QCM-based biosensors for the detection of infectious diseases are extensively reviewed, with a focus on prevalent diseases for which improved diagnostic techniques are in high demand. The challenges to the clinical application of QCM-based biosensors are highlighted, along with an outline of the future scope of research in QCM-based diagnostics.

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Integration of a Miniature Quartz Crystal Microbalance with a Microfluidic Chip for Amyloid Beta-Aβ42 Quantitation

Cited by 19 publications

References 32 publications

Acoustic Immunosensing of Exosomes Using a Quartz Crystal Microbalance with Dissipation Monitoring

Acoustic Immunosensing of Exosomes Using a Quartz Crystal Microbalance with Dissipation Monitoring

The Microfluidic Toolbox for Analyzing Exosome Biomarkers of Aging

Quartz crystal microbalance-based biosensors as rapid diagnostic devices for infectious diseases

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