Integration of ABA, GA, and light signaling in seed germination through the regulation of ABI5

Zhao, Hongyun; Zhang, Yamei; Zheng, Yuan

doi:10.3389/fpls.2022.1000803

Cited by 30 publications

(19 citation statements)

References 101 publications

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“…This indicates that the exogenous hormone ABA might inhibit the elongation growth of the embryo and prevent the space for embryo development from expanding during the after-ripening process. ABA positively regulates seed dormancy, while GA antagonizes ABA to promote seed germination (Chen et al, 2020;Abley et al, 2021;Zhao et al, 2022). Endogenous ABA is accumulated in A. thaliana seeds during the maturation process, which induces and maintains the dormancy of seeds, and then GA content is upregulated to release dormancy during the after-ripening process (Shu et al, 2016b).…”

Section: Discussionmentioning

confidence: 99%

“…ABA positively regulates seed dormancy, while GA antagonizes ABA to promote seed germination ( Chen et al., 2020 ; Abley et al., 2021 ; Zhao et al., 2022 ). Endogenous ABA is accumulated in A. thaliana seeds during the maturation process, which induces and maintains the dormancy of seeds, and then GA content is upregulated to release dormancy during the after-ripening process ( Shu et al., 2016b ).…”

Section: Discussionmentioning

confidence: 99%

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Exogenous abscisic acid prolongs the dormancy of recalcitrant seed of Panax notoginseng

Wang

Yang

et al. 2023

Front. Plant Sci.

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The seeds of Panax notoginseng (Burk.) F. H. Chen are typically characterized by their recalcitrance and after-ripening process and exhibit a high water content at harvest as well as a high susceptibility to dehydration. Storage difficulty and the low germination of recalcitrant seeds of P. notoginseng are known to cause an obstacle to agricultural production. In this study, the ratio of embryo to endosperm (Em/En) in abscisic acid (ABA) treatments (1 mg·l−1 and 10 mg·l−1, LA and HA) was 53.64% and 52.34%, respectively, which were lower than those in control check (CK) (61.98%) at 30 days of the after-ripening process (DAR). A total of 83.67% of seeds germinated in the CK, 49% of seeds germinated in the LA treatment, and 37.33% of seeds germinated in the HA treatment at 60 DAR. The ABA, gibberellin (GA), and auxin (IAA) levels were increased in the HA treatment at 0 DAR, while the jasmonic acid (JA) levels were decreased. ABA, IAA, and JA were increased, but GA was decreased with HA treatment at 30 DAR. A total of 4,742, 16,531, and 890 differentially expressed genes (DEGs) were identified between the HA-treated and CK groups, respectively, along with obvious enrichment in the ABA-regulated plant hormone pathway and the mitogen-activated protein kinase (MAPK) signaling pathway. The expression of pyracbactin resistance-like (PYL) and SNF1-related protein kinase subfamily 2 (SnRK2s) increased in the ABA-treated groups, whereas the expression of type 2C protein phosphatase (PP2C) decreased, both of which are related to the ABA signaling pathway. As a result of the changes in expression of these genes, increased ABA signaling and suppressed GA signaling could inhibit the growth of the embryo and the expansion of developmental space. Furthermore, our results demonstrated that MAPK signaling cascades might be involved in the amplification of hormone signaling. Meanwhile, our study uncovered that the exogenous hormone ABA could inhibit embryonic development, promote dormancy, and delay germination in recalcitrant seeds. These findings reveal the critical role of ABA in regulating the dormancy of recalcitrant seeds, and thereby provide a new insight into recalcitrant seeds in agricultural production and storage.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Exogenous abscisic acid prolongs the dormancy of recalcitrant seed of Panax notoginseng

Wang

Yang

et al. 2023

Front. Plant Sci.

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“…The Co-IP assay followed a previously described method (Nie et al, 2022; Zhan et al, 2023). Briefly, the total proteins for CAR1-mCherry/HAB1-mGFP5, CAR1-mCherry/HAB2-mGFP5, CAR1-mCherry/SnRK2.2-mGFP5, CAR1-mCherry/VAMP724-mGFP5, and CAR1-mCherry/FLOT1-mGFP5 transgenic plants were extracted and incubated with anti-GFP beads (Sigma-Aldrich) for 2 h at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Role of C2-domain protein CAR1 in the plasma membrane ABA signaling response

Guo,

Wu,

Bai

et al. 2023

Preprint

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Plasma membrane (PM)-associated abscisic acid (ABA) signal transduction is an important component of ABA signaling. The C2-domain ABA-related (CAR) proteins have been reported to play a crucial role in recruiting ABA receptor PYR1/PYL/RCAR (PYLs) to the PM. However, the molecular details of the involvement of CAR proteins in membrane-delimited ABA signal transduction remain unclear. Here, the GUS-tagged materials for all Arabidopsis CAR members were used to comprehensively visualize the extensive expression patterns of the CAR family genes. Based on the specificity of CAR1's response to ABA, we determined to use it as a target to study the function of CAR proteins in PM-associated ABA signaling. Single-particle tracking showed that ABA affected the spatiotemporal dynamics of CAR1. The presence of ABA prolonged the dwell time of CAR1 on the membrane and showed faster lateral mobility. Surprisingly, we verified that CAR1 could directly recruit Hypersensitive to ABA1 (HAB1) and SNF1-related protein kinase 2.2 (SnRK2.2) to the PM at both the bulk and single-molecule levels. Furthermore, PM localization of CAR1 was demonstrated to be related to membrane microdomains. Collectively, our study revealed that CARs recruited the three main components of ABA signaling to the PM to respond positively to ABA. This study deepens our understanding of ABA signal transduction.

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“…Secara umum kandungan GA 3 lebih tinggi dibandingkan ABA. Selama proses perkecambahan kandungan ABA dalam benih menurun (Liu et al, 2014;Carrillo-Barral et al, 2020) sementara kandungan GA 3 meningkat sehingga dapat menyebabkan terjadinya perkecambahan (Zhao et al, 2022).…”

Section: Hasil Dan Pembahasanunclassified

Karakter Agro-morfologi dan Periode After-ripening Benih Padi Lanras Lokal Potensial asal Timor-Leste

BRANCO

Ilyas

Purwoko

et al. 2022

J. Agron. Indonesia

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Benih padi yang baru dipanen umumnya tidak berkecambah walaupun dikecambahkan pada kondisi optimum yang disebut after-ripening, dan secara alamiah akan hilang setelah waktu tertentu. Penelitian ini bertujuan melakukan karakterisasi agro-morfologi dan menetapkan periode after-ripening benih 5 lanras potensial di Timor-Leste. Penelitian dilaksanakan pada bulan April 2018-November 2019. Penanaman dan pengamatan karakter agro-morfologi 5 lanras potensial Timor-Leste, yaitu Hare R-oitu, Fos Mean, Hare Belit, Hare Nona Portu, dan Ale Mamea Ula Lesa, dilaksanakan di Desa Caibada Distrik Baucau, Timor-Leste. Pengamatan periode after ripening dan mutu benih ke lima lanras tersebut dilaksanakan di Laboratorium Pengujian dan Penyimpanan Benih, Departemen Agronomi dan Hortikultura, IPB University, dan dirancang dalam rancangan acak lengkap (RAL) dua faktor dengan empat ulangan. Faktor pertama adalah 5 lanras tersebut di atas dan faktor kedua adalah periode simpan yang terdiri atas 2, 3, 4, 5, 6, 7, dan 8 minggu setelah panen (MSP). Hasil penelitian menunjukkan bahwa 5 lanras potensial di Tinmor-Leste mempunyai keragaan tanaman yang pendek (76.3-105.50 cm), tergolong berumur sedang (129-141 HSS), dan jumlah anakan produktif tinggi (17.5-53.50 anakan), kecuali Ale Mamea Ula Lesa. Lima lanras tersebut mempunyai potensi produktivitas yang tinggi berkisar 8.1-9.8 ton ha-1. Periode after-ripening lanras Hare Belit dan Hare Nona Portu berakhir pada 2 MSP, Hare R-oitu pada 6 MSP, sedangkan Ale Mamea Ula Lesa dan Fos Mean pada 8 MSP. Semakin lama benih disimpan rasio kandungan ABA/GA3 semakin menurun dari 0.53-1.22 ppm pada 2 MSP menjadi 0.45-0.93 ppm pada 4 MSP. Lanras Hare Belit dan Hare Nona Portu merupakan lanras yang potensial dikembangkan menjadi varietas nasional di Timor-Leste, masing-masing untuk pengembangan di dataran rendah dan dataran sedang. Kata kunci: karakterisasi, intensitas dormansi, persentase perkecambahan, mutu benih

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Integration of ABA, GA, and light signaling in seed germination through the regulation of ABI5

Cited by 30 publications

References 101 publications

Exogenous abscisic acid prolongs the dormancy of recalcitrant seed of Panax notoginseng

Exogenous abscisic acid prolongs the dormancy of recalcitrant seed of Panax notoginseng

Role of C2-domain protein CAR1 in the plasma membrane ABA signaling response

Karakter Agro-morfologi dan Periode After-ripening Benih Padi Lanras Lokal Potensial asal Timor-Leste

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