Integration of adeno-associated virus 2 DNA in human MKR melanoma cells induces a peptide with oncosuppressive properties

Bantel-Schaal, Ursula

doi:10.1002/ijc.1230

Cited by 4 publications

(3 citation statements)

References 37 publications

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“…Based on our present data, we are unable to describe the precise molecular mechanism underlying the observed interference between the viral infection and drug action. Induction and release of an “AAV‐induced factor” have been described in a human melanoma cell line harboring AAV‐2 28. This finding supports our hypothesis that AAV infection might induce chemokine‐like factors in vivo as well.…”

Section: Discussionsupporting

confidence: 89%

Prevention of chemotherapy‐related toxic side effects by infection with adeno‐associated virus type 2

Eisold

Dihlmann

Linnebacher

et al. 2002

Intl Journal of Cancer

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Section: Discussionsupporting

confidence: 89%

Prevention of chemotherapy‐related toxic side effects by infection with adeno‐associated virus type 2

Eisold

Dihlmann

Linnebacher

et al. 2002

Intl Journal of Cancer

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“…This is because oncoviruses are necessary but possibly not sufficient for carcinogenesis. Literature reviews have suggested that the pathogenicity of AAV2 in cancers should be determined based on context-dependent interpretations, such as the cell specificity, interaction with other risk factors, and immune status of the host [ 4 5 6 8 ]. Indeed, the immune system of the host can play dual roles in carcinogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…To date, AAV2 has been considered a nonpathogenic virus because it requires a helper virus, such as adenovirus, for productive infection. Furthermore, several previous studies have demonstrated the oncosuppressive role of AAV2 infection in some cancer cell lines, such as human papillomavirus-positive cervical cancers, MKr melanoma, and breast cancer [ 4 5 6 ]. In contrast, few studies have examined the possibility of hepatocarcinogenesis after AAV vector-mediated gene therapy in mice [ 7 8 ].…”

Section: Introductionmentioning

confidence: 99%

Adeno-Associated Virus 2-Mediated Hepatocellular Carcinoma is Very Rare in Korean Patients

et al. 2016

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BackgroundThe incidence and etiology of hepatocellular carcinoma (HCC) vary widely according to race and geographic regions. The insertional mutagenesis of adeno-associated virus 2 (AAV2) has recently been considered a new viral etiology of HCC. The aim of this study was to investigate the frequency and clinical characteristics of AAV2 in Korean patients with HCC.MethodsA total of 289 unrelated Korean patients with HCC, including 159 Hepatitis-B-related cases, 16 Hepatitis-C-related cases, and 114 viral serology-negative cases, who underwent surgery at the Samsung Medical Center in Korea from 2009 to 2014 were enrolled in this study. The presence of AAV2 in fresh-frozen tumor tissues was investigated by DNA PCR and Sanger sequencing. The clinical and pathological characteristics of AAV2-associated HCC in these patients were compared with previous findings in French patients.ResultsThe AAV2 detection rate in Korean patients (2/289) was very low compared with that in French patients (11/193). Similar to the French patients, the Korean patients with AAV2-related HCC showed no signs of liver cirrhosis. The Korean patients were younger than the French patients with the same AAV2-associated HCC; the ages at diagnosis of the two Korean patients were 47 and 39 yr, while the median age of the 11 French patients was 55 yr (range 43-90 yr).ConclusionsAAV2-associated HCC was very rare in Korean patients with HCC. Despite a limited number of cases, this study is the first to report the clinical characteristics of Korean patients with AAV2-associated HCC. These findings suggest epidemiologic differences in viral hepatocarcinogenesis between Korean and European patients.

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Adenoassociated Virus Type 2-Induced Inhibition of the Human Papillomavirus Type 18 Promoter in Transgenic Mice

et al. 2002

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The epithelium of the cervix uteri has been reported to be frequently coinfected with both human papillomaviruses (HPV) and helper virus-dependent adenoassociated viruses (AAV). Seroepidemiological data suggest that AAV infection could inhibit cervical cancer that is caused by specific ("high-risk") types of papillomaviruses. In vitro, infection with AAV type 2 (AAV-2) or transfection of AAV-2 early (rep) genes has been shown to inhibit transformation by papillomaviruses. To analyze the effects of AAV on HPV in vivo, we studied the influence of AAV-2 infection on the promoter activity of high-risk HPV type 18 (HPV-18) in mice, transgenic for sequences of the upstream regulatory region (URR) of HPV-18 controlling transcription of the reporter gene, lacZ. Transgenic animals (or tongue cells thereof, explanted and grown in culture) were treated with dexamethasone to induce the HPV-18 promoter. Simultaneously they were (i) infected with AAV, (ii) inoculated with AAV virus-like particles (VLPs; empty capsids), or (iii) mock infected. Inoculation with AAV-2 or VLPs inhibited activation of the HPV-18 promoter. In vitro, in baby hamster kidney cells transfected with the HPV-18-lacZ construct, tissue extracts from AAV-infected animals suppressed the HPV-18 URR to a similar extent as AAV infection did. Down-regulation of the HPV-18 promoter was less efficient with extracts from animals inoculated with VLPs and was not observed with extracts from uninfected or dexamethasone-treated animals. This indicates that AAV induces cellular factor(s) in vivo capable of mediating down-regulation of the HPV-18 promoter also in cells in vitro. In contrast, promoters of the low-risk HPV types (HPV-6, HPV-11) were not influenced by AAV infection as opposed to promoters of the high-risk types (HPV-18 and HPV-16).

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Integration of adeno-associated virus 2 DNA in human MKR melanoma cells induces a peptide with oncosuppressive properties

Cited by 4 publications

References 37 publications

Prevention of chemotherapy‐related toxic side effects by infection with adeno‐associated virus type 2

Prevention of chemotherapy‐related toxic side effects by infection with adeno‐associated virus type 2

Adeno-Associated Virus 2-Mediated Hepatocellular Carcinoma is Very Rare in Korean Patients

Adenoassociated Virus Type 2-Induced Inhibition of the Human Papillomavirus Type 18 Promoter in Transgenic Mice

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