Integration of biochemical and topographic cues for the formation and spatial distribution of invadosomes in nasopharyngeal epithelial cells

Using Biomimetic Scaffold Platform to Detect Growth Factor Induced Changes in Migration Dynamics of Nasopharyngeal Epithelial Cells

“…The NP460 cells with or without TGF-β1 treatment at a density of 8-10 x 10 4 cells/ml were seeded onto the platforms. After incubating the cells at 37 °C with 5% CO2 supply for 6 h for the initial attachment, the culturing medium was removed and replaced as reported previously [18] by a mixture of NP460 cell culture medium and CO2 independent medium (Gibco) with 10% fetal bovine serum, 1% GlutaMAX supplement and 1% P/S in 1:1 (v/v) ratio. An upright microscope (Nikon Eclipse NI-U) with a 20X objective together with a 37 °C incubator without CO2 supply was used to capture time-lapse images of cells on the two-layer scaffold platforms every 5 min for 15 h. NIH ImageJ with manual tracking plugin (version 1.50i) was used to analyze the cell migration time-lapse images.…”

“…As a proof of concept, the present study aimed to investigate the feasibility of detecting the local epithelialto-mesenchymal transition (EMT) induced by the environmental conditions on the migration kinetics of cultured cells through the use of an engineered platform. In Southern China and Southeast Asia, nasopharyngeal carcinoma (NPC) is one of the most common and highly invasive carcinomas [16][17][18]. It has been reported that transforming growth factor beta-1 (TGF-β1) upregulates the formation of invadosomes of nasopharyngeal cells which are responsible for cell invasion and metastasis while the serum of NPC patients normally have higher TGF-β1 level [18].…”

“…In Southern China and Southeast Asia, nasopharyngeal carcinoma (NPC) is one of the most common and highly invasive carcinomas [16][17][18]. It has been reported that transforming growth factor beta-1 (TGF-β1) upregulates the formation of invadosomes of nasopharyngeal cells which are responsible for cell invasion and metastasis while the serum of NPC patients normally have higher TGF-β1 level [18]. TGF-β1 is an inflammatory cytokine, which is present in many biological activities.…”

“…It can suppress the tumor progression in early stage, but it also promotes tumor progression [19,20]. In addition, the treatment of untransformed nasopharyngeal epithelial cells, such as the NP460 cell line [18], with TGF-β1 is known to trigger invadosome formation and EMT, an important process for tumor progression, usually associated with the loss of cellcell adhesion, changes in cell-matrix interaction, and enhancement of cell migration and invasion [21,22]. In this project, the migration and traversing behaviors of NP460 cells, with the cell size of 15-20 μm, on engineered doublelayer scaffold platforms were studied.…”

Microenvironmental topographic cues influence migration dynamics of nasopharyngeal carcinoma cells from tumour spheroids

“…3 Tumour metastasis is inuenced by a combination of biochemical and physical cues in the surrounding microenvironment. 4 Many model systems have been established to mimic the physical tumour environment and study how the architectural information of the extracellular milieu controls cancer cell migration. We and others have demonstrated that, whereas cells move randomly on at surfaces, their motility and directionality could be guided by topographical patterns on engineered surfaces [5][6][7][8][9] and microchannels.…”