Integration of biocomponents with synthetic structures: use of conducting polymer polyelectrolyte composites

Hodgson, A.J.; John, Melinda J.; Campbell, Toni E.; Georgevich, A.; Woodhouse, S.; Aoki, Takashi; Ogata, Naoya; Wallace, Gordon G.

doi:10.1117/12.232137

Cited by 43 publications

(43 citation statements)

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“…Table 2 summarizes various techniques reported for the production of neurotrophin-modified conducting polymers with their important characteristics including electrical properties. Mostly, non-covalent methods have been developed, which include doping NGF/NT-3 during the electrochemical synthesis of PPy (Hodgson et al 1996;Kim et al 2007;Richardson et al 2007) and coupling biotinylated NGF on avidin-treated biotin-doped PPy films (George et al 2006). These non-conjugation methods showed good conductivities and controllable NGF release profiles in response to an external electrical stimulation to induce neurite formation from PC12 cells.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, external electrical potentials were applied to the NGF-PPy-NSE 50 films, conditions which were used to release NGF from NGF-doped PPy films as described by others (Hodgson et al 1996;George et al 2006). Minimal amount of NGF (less than 15 pg cm K2 film) was detected after applying K1 V for 5 min.…”

Section: Stability Of Immobilized Ngf Onmentioning

confidence: 99%

“…Three samples were analysed for each condition. Electrical potentials were applied to NGFimmobilized PPy-NSE 50 samples to study the stabilities of NGF links and neurotrophic activities of the films according to previous literature (Hodgson et al 1996;George et al 2006). A constant reducing potential, K1 V versus SCE, was applied to the NGFPPy-NSE 50 films in PBS buffer for 5 min with a Pt counter electrode.…”

Section: Stability Of Immobilized Ngf On Thementioning

confidence: 99%

“…However, the lack offunctional groups on PPy has limited the ability to tailor PPy with various biochemical properties (Lee et al 2006;Song et al 2006;Guimard et al 2007). A number of non-covalent modification techniques have been studied, which involve doping and entrapping different molecules, such as hyaluronic acid (Collier et al 2000), heparin (Li et al 2005), laminin fragments (Cui et al 2003;Stauffer & Cui 2006) and nerve growth factor (NGF; Hodgson et al 1996;Kim et al 2007) during PPy polymerization. Also, affinity peptides that have specific binding to chloride-doped PPy were used to tether RGD peptides and to promote PC12 adhesion (Sanghvi et al 2005).…”

Section: Introductionmentioning

confidence: 99%

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Neuroactive conducting scaffolds: nerve growth factor conjugation on active ester-functionalized polypyrrole

Lee

Schmidt

2008

J. R. Soc. Interface.

101

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Electrically conductive and biologically active scaffolds are desirable for enhancing adhesion, proliferation and differentiation of a number of cell types such as neurons. Hence, the incorporation of neuroactive molecules into electroconductive polymers via a specific and stable method is essential for neuronal tissue engineering applications. Traditional conjugation approaches dramatically impair conductivities and/or stabilities of the scaffolds and ligands. In this study, we developed copolymers (PPy-NSE) of N-hydroxyl succinimidyl ester pyrrole and regular pyrrole, which can be immobilized with nerve growth factor (NGF) without significantly hindering electroconductivity. The presence of active ester groups was confirmed using reflectance infrared spectroscopy and X-ray photoelectron spectroscopy (XPS) from the copolymers prepared from different monomer compositions. We selected PPy-NSE 50 (polymerized from a 50 : 50 monomer ratio of pyrrole : pyrrole-NSE) for further modification with NGF because this copolymer retains good conductivity (approx. 8 S cm K1 ) and presents active ester groups for NGF immobilization. We tethered NGF on the PPy-NSE 50 surface, and found that PC12 cells extended neurites similarly to cells cultured in NGF-containing medium. XPS and enzyme-linked immunosorbent assay confirmed that NGF immobilized via the active ester on the PPy-NSE 50 film was stable for up to 5 days in phosphate-buffered saline solution. Also, application of an external electrical potential to NGF-immobilized PPy films did not cause a significant release of NGF nor reduce their neurotrophic activity. This novel scaffold, providing electroconductive and neurotrophic activities, has potential for neural applications, such as tissue engineering scaffolds and biosensors.

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Section: Discussionmentioning

confidence: 99%

Section: Stability Of Immobilized Ngf Onmentioning

confidence: 99%

Section: Stability Of Immobilized Ngf On Thementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Neuroactive conducting scaffolds: nerve growth factor conjugation on active ester-functionalized polypyrrole

Lee

Schmidt

2008

J. R. Soc. Interface.

101

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“…The thickness and morphology of the film are primarily controlled by the amount of electric charge passed between the counter and working electrodes. Additionally, biofunctionality can be easily imparted to CPs during electrodeposition through the incorporation of biological entities such as silk-like protein fragments (SLPF) [59], hyaluronic acid (HA) [60,61], various laminin peptides [1,2,59,62], enzymes [28,[63][64][65][66], polymeric amino acids [67,68], growth factors [2,11,69,70] and whole cells [71]. Electrodeposition is the most commonly used method for coating conventional metallic bioelectrodes.…”

Section: Conventional Cp Fabricationmentioning

confidence: 99%

Conducting polymer-hydrogels for medical electrode applications

Green

Baek

Poole-Warren

et al. 2010

Science and Technology of Advanced Materials

247

182

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Conducting polymers hold significant promise as electrode coatings; however, they are characterized by inherently poor mechanical properties. Blending or producing layered conducting polymers with other polymer forms, such as hydrogels, has been proposed as an approach to improving these properties. There are many challenges to producing hybrid polymers incorporating conducting polymers and hydrogels, including the fabrication of structures based on two such dissimilar materials and evaluation of the properties of the resulting structures. Although both fabrication and evaluation of structure-property relationships remain challenges, materials comprised of conducting polymers and hydrogels are promising for the next generation of bioactive electrode coatings.

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Polypyrrole-heparin composites as stimulus-responsive substrates for endothelial cell growth

Garner

Georgevich

Hodgson

et al. 1999

J. Biomed. Mater. Res.

Self Cite

265

143

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Heparin is a potent anticoagulant which can be immobilized on biomaterial surfaces to increase their hemocompatability. In the present work, we have electrochemically synthesized composites comprising heparin and the electrically conducting polymer polypyrrole. The incorporation and exposure of heparin were controlled by varying key conditions of polymer synthesis (i.e., applied current and synthesis time). The resulting composite polymers were electroactive after synthesis and the amount of heparin exposed in the polymer could be increased (up to threefold) by switching the polymers from their oxidized to reduced states. Polymer reduction was achieved by either application of negative potentials (-0.4 to -0.7 V for 90 s) or exposure to aqueous reductant (0.1M sodium dithionite for 30 min). Heparin-polypyrrole composites remained stable after autoclaving, displaying no significant loss of electroactivity, and had a shelf life of at least 2 years postautoclaving. Finally, the composites were found to be excellent substrates for the growth of human endothelial cells.

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Integration of biocomponents with synthetic structures: use of conducting polymer polyelectrolyte composites

Cited by 43 publications

References 0 publications

Neuroactive conducting scaffolds: nerve growth factor conjugation on active ester-functionalized polypyrrole

Neuroactive conducting scaffolds: nerve growth factor conjugation on active ester-functionalized polypyrrole

Conducting polymer-hydrogels for medical electrode applications

Polypyrrole-heparin composites as stimulus-responsive substrates for endothelial cell growth

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