Integration of common bean (Phaseolus vulgaris L.) linkage and chromosomal maps

Pedrosa, Andrea; Vallejos, C. Eduardo; Bachmair, Andreas; Schweizer, Dieter

doi:10.1007/s00122-002-1138-3

Cited by 71 publications

(65 citation statements)

References 42 publications

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“…Pedrosa-Harand, unpublished data). Chromosome 4 was previously named 10 (Pedrosa et al 2003), but all chromosomes have been recently renamed according to the linkage group names of the core linkage map of common bean (Freyre et al 1998;Pedrosa-Harand et al 2008).…”

Section: Resultsmentioning

confidence: 99%

Molecular Analysis of a Large Subtelomeric Nucleotide-Binding-Site–Leucine-Rich-Repeat Family in Two Representative Genotypes of the Major Gene Pools of Phaseolus vulgaris

et al. 2009

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In common bean, the B4 disease resistance (R) gene cluster is a complex cluster localized at the end of linkage group (LG) B4, containing at least three R specificities to the fungus Colletotrichum lindemuthianum. To investigate the evolution of this R cluster since the divergence of Andean and Mesoamerican gene pools, DNA sequences were characterized from two representative genotypes of the two major gene pools of common bean (BAT93: Mesoamerican; JaloEEP558: Andean). Sequences encoding 29 B4-CC nucleotide-binding-site-leucine-rich-repeat (B4-CNL) genes were determined-12 from JaloEEP558 and 17 from BAT93. Although sequence exchange events were identified, phylogenetic analyses revealed that they were not frequent enough to lead to homogenization of B4-CNL sequences within a haplotype. Genetic mapping based on pulsed-field gel electrophoresis separation confirmed that the B4-CNL family is a large family specific to one end of LG B4 and is present at two distinct blocks separated by 26 cM. Fluorescent in situ hybridization on meiotic pachytene chromosomes revealed that two B4-CNL blocks are located in the subtelomeric region of the short arm of chromosome 4 on both sides of a heterochromatic block (knob), suggesting that this peculiar genomic environment may favor the proliferation of a large R gene cluster.

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Section: Resultsmentioning

confidence: 99%

Molecular Analysis of a Large Subtelomeric Nucleotide-Binding-Site–Leucine-Rich-Repeat Family in Two Representative Genotypes of the Major Gene Pools of Phaseolus vulgaris

et al. 2009

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“…Although the relationship between physical and map distances is known to vary greatly throughout the genome (Dooner and Martínez-Férez 1997;Wei et al 1999;Fridman et al 2000), it is also known that this relationship decreases significantly near the telomeres (Gill and Friebe 1998). Given the distal position of the I locus on chromosome 9 (formerly known as linkage group D; Pedrosa et al 2003), the physical distance between these two loci was expected to be closer to the lower end of the estimated range. At this distance, the construction and alignment of linkage and physical maps appeared feasible.…”

Section: Resultsmentioning

confidence: 99%

“…The length of the gap is unknown. The other three contigs each had a single TIR-hybridizing fragment and mapped to chromosomes 4, 5, and 9 (formerly known as linkage groups A, C, and D; Pedrosa et al 2003). The latter was distantly linked to the I locus.…”

Section: Resultsmentioning

confidence: 99%

Genetic and Molecular Characterization of the I Locus of Phaseolus vulgaris

et al. 2006

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The I locus of the common bean, Phaseolus vulgaris, controls the development of four different phenotypes in response to inoculation with Bean common mosaic virus, Bean common mosaic necrosis virus, several other related potyviruses, and one comovirus. We have generated a high-resolution linkage map around this locus and have aligned it with a physical map constructed with BAC clones. These clones were obtained from a library of the cultivar ''Sprite,'' which carries the dominant allele at the I locus. We have identified a large cluster of TIR-NBS-LRR sequences associated within this locus, which extends over a distance .425 kb. Bean cultivars from the Andean or Mesoamerican gene pool that contain the dominant allele share the same haplotypes as revealed by gel blot hybridizations with a TIR probe. In contrast, beans with a recessive allele display simpler and variable haplotypes. A survey of wild accessions from Argentina to Mexico showed that this multigene family has expanded significantly during evolution and domestication. RNA gel blot analysis indicated that the TIR family of genes plays a role in the response to inoculations with BCMV or BCMNV.

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“…Variation in chromosome patterns of 35S rRNA gene sites has been observed in many different plant species such as Oryza sativa subsp. japonica [35], Centaurea jacea [36] and Phaseolus species [37]. Variable loci of 5S rDNA have been reported, inter alia, in A. thaliana [7] and Brassica juncea var.…”

Section: Resultsmentioning

confidence: 98%

Intraspecific polymorphism of ribosomal DNA loci number and morphology in Brachypodium pinnatum and Brachypodium sylvaticum

Breda

Wolny

Hasterok

2012

Cellular and Molecular Biology Letters

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Abbreviations used: Ag-NOR -silver-binding nucleolar organizer region; BAC -bacterial artificial chromosome; CMA+ -chromomycin A 3 positive bands; FISH -fluorescence in situ hybridisation; GISH -genomic in situ hybridisation; ITS -internal transcribed spacer; NOR -nucleolar organizer region; NTS -non-transcribed spacer; rDNA -ribosomal DNA Abstract: The genus Brachypodium has become the target of extensive cytomolecular studies since one of its representatives, B. distachyon, has been accepted as a model plant for temperate cereals and forage grasses. Recent preliminary studies suggested that intraspecific rDNA polymorphism can occur in at least two members of the genus, B. sylvaticum and B. pinnatum, so the aim of this study was to further analyse this phenomenon. FISH with 25S rDNA and 5S rDNA probes was performed on somatic metaphase chromosomes, supplemented by the silver staining technique which distinguishes transcriptionally active from inactive 18S-5.8S-25S rDNA loci. The number, size and chromosomal distribution of 5S rDNA loci were very constant: two loci were invariably observed in all studied diploid accessions of both species, while four 5S rDNA loci were present in the tetraploid B. pinnatum. In contrast to 5S rDNA loci, those of the 35S rDNA were more variable. Two or three loci were observed in the diploid B. pinnatum and four in tetraploid accessions. In chromosome complements of B. sylvaticum accessions from two to six 35S rDNA sites were detected. Regardless of total rDNA locus number, only two were transcriptionally active in diploid accessions of both species, while two or four were active in the tetraploid B. pinnatum. Additionally, the fluorescent CMA/DAPI banding method was used to identify the relation between rDNA sites and CMA+ bands. It was revealed that the number and chromosomal distribution of CMA+ bands are in congruence only with 35S rDNA loci which gave strong FISH signals.

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Integration of common bean (Phaseolus vulgaris L.) linkage and chromosomal maps

Cited by 71 publications

References 42 publications

Molecular Analysis of a Large Subtelomeric Nucleotide-Binding-Site–Leucine-Rich-Repeat Family in Two Representative Genotypes of the Major Gene Pools of Phaseolus vulgaris

Molecular Analysis of a Large Subtelomeric Nucleotide-Binding-Site–Leucine-Rich-Repeat Family in Two Representative Genotypes of the Major Gene Pools of Phaseolus vulgaris

Genetic and Molecular Characterization of the I Locus of Phaseolus vulgaris

Intraspecific polymorphism of ribosomal DNA loci number and morphology in Brachypodium pinnatum and Brachypodium sylvaticum

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