Integration of cytochromecoxidase I barcodes and geometric morphometrics to delimit species in the genusGnopharmia(Lepidoptera: Geometridae, Ennominae)

Abstract

We tested the hypothesis of species taxonomy in the genus Gnopharmia (Macariini, Ennominae) that was recently established in a review based on discrete morphological characters. For this objective we integrated both DNA‐based and morphometric approaches in order to infer species boundaries. A 658‐bp fragment of the mitochondrial cytochrome c oxidase subunit 1 (CO1) (DNA barcode) was analysed from populations of five species distributed throughout the Middle East to assess their consistency with traditionally defined morphospecies. Signals in the morphological variation of the aedeagus of all relevant populations were evaluated using geometric landmarks. Consistent groupings compatible with the current taxonomic classification were found with both approaches. The results strongly support the distinction of seven closely related species. © 2013 The Linnean Society of London

A new cryptic species of Nagiella Munroe from China revealed by DNA barcodes and morphological evidence (Lepidoptera, Crambidae, Spilomelinae)

“…Recently the integration of DNA barcoding and morphological approaches opened the field for researchers in accelerating species identification and assisted in detecting previously undetected cryptic species (Sutrisno 2005; Mutanen et al 2012; Haines & Rubinoff 2012; Yang et al 2012; Rajaeish et al 2013; Yang et al 2016; Mally et al 2016). The taxonomic placement of N. occultalis sp.…”

A new species of Cymodoce Leach, 1814 (Crustacea: Isopoda: Sphaeromatidae) based on morphological and molecular data, with a key to the Northern Indian Ocean species

“…Nevertheless, the barcode fragments were AT rich (A = 26%, C = 17%, G = 20% and T = 37%), as it is typically known from this gene fragment for arthropods (e.g. Simon et al 2006;Raupach et al 2010;Wesener et al 2010;Rajaei et al 2013). All specimens of the same species grouped together (Fig.…”

Geometric Morphometrics Approach on Genotoxicity Evaluation of Ecdysone Agonist, Chromafenozide on Corcyra Cephalonica Stainton (Lepidoptera: Pyralidae)

“…Currently, it is well known that the morphological variations of insects can be detected using morphometric analysis as a tool for quantitative and multidimensional comparison of morphological characters. Landmark-based geometric morphometrics allows the evaluation of phenotypic differentiations and has a high discriminatory power at species and even subspecies level [14]. In the present study, the egg masses of C. cephalonica were exposed to the treatment of ecdysone agonist, chromafenozide to evaluate the genotoxicity effect of the compound on the wing architecture using landmark-based geometric morphometrics.…”