Integration of cytogenetic and genetic linkage maps of Lotus japonicus, a model plant for legumes

Ohmido, Nobuko; Ishimaru, Akiko; Kato, Seiji; Sato, Shusei; Tabata, Satoshi; Fukui, Kiichi

doi:10.1007/s10577-009-9103-5

Cited by 33 publications

(30 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With the construction of a genetic linkage map in Passiflora (Carneiro et al, 2002;Oliveira et al, 2008), genetic sequences of agronomic importance could be located on the chromosomes by the FISH technique, resulting in the integration of the cytogenetic and genetic linkage map, as noted in Lotus japonicus (Ohmido et al, 2010). With the utilization of Passiflora interspecific hybrids in the ornamental plant market (Abreu et al, 2009), the use of the GISH technique, still unpublished for this genus, is promising with regard to the identification of introgression of chromosomes or chromosomal fragments.…”

Section: Resultsmentioning

confidence: 99%

Improvements in cytological preparations for fluorescent in situ hybridization in Passiflora

Souza¹,

Urdampilleta²,

Forni‐Martins³

2010

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Cytological preparations for the fluorescent in situ hybridization (FISH) technique require cytoplasm-free metaphases, with well-spread chromosomes, for the localization of DNA sequences and chromosome mapping. We tested various procedures for FISH analysis of Passiflora cacaoensis, P. gardneri and hybrid F 1 progeny of P. gardneri x P. gibertii. Two treatments with four enzymes and three incubation times were compared. The material was treated with 1.0 M HCl before enzymatic digestion. The following criteria were used to determine the quality of the metaphases: a) lack or presence of cytoplasm; b) well-spread chromosomes or with overlap; c) complete or incomplete chromosome number (2n). The enzyme Pectinex ® SP ULTRA gave the best performance, with the shortest incubation time. The best results were observed after 30 min of incubation; more than 70% of the metaphases did not have large amounts of cytoplasm or overlapping chromosomes, and about 75% maintained the chromosome number. FISH was carried out using a 45S rDNA probe (pTa71) labeled with biotin and detected with fluorescein isothiocyanate. Sites with strong staining and without 2149©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 9 (4): 2148-2155 (2010) Cytological preparations for FISH in Passiflora nonspecific signals were observed. Our methodological adaptations allowed the preparation of metaphase slides of high quality for the FISH technique, with less time required for the preparation of samples.

show abstract

Section: Resultsmentioning

confidence: 99%

Improvements in cytological preparations for fluorescent in situ hybridization in Passiflora

Souza¹,

Urdampilleta²,

Forni‐Martins³

2010

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…The 5S rDNA and telomere repeat sequences were labeled by PCR with biotin-16-dUTP or digoxigenin-11-dUTP (Roche, Branchburg, N.J., USA), and the 16 BAC clones and 26S rDNA sequence were labeled using a Nick Translation kit (Roche) with biotin-16-dUTP or digoxigenin-11-dUTP according to the method described by Ohmido et al [2010].…”

Section: Dna Probesmentioning

confidence: 99%

“…FISH analysis using the 2 rRNA genes, the telomeric repeat, and the BAC clones was performed using chromosome spreads carefully prepared according to the method described by Ohmido et al [2010]. For immunocytochemical fluorescence detection, the slides were incubated with fluorescein isothiocyanate (FITC)-conjugated anti-digoxigenin antibody (Roche) and streptavidin-conjugated Cy3 (Cy3-SA; Jackson ImmunoResearch Laboratories, West Grove, Pa., USA) in 4 !…”

Section: Fluorescence In Situ Hybridizationmentioning

confidence: 99%

“…Previous reports have described a comparative chromosome map comprising genetic linkage and physical maps for the autogamous legume L. japonicus [Sato et al, 2008;Ohmido et al, 2010]. In contrast to L. japonicus , red clover is allogamous, self-incompatible, and broadly polymorphic in its population and progeny.…”

Section: Integration Of Red Clover Linkage and Chromosome Mapsmentioning

confidence: 99%

See 1 more Smart Citation

Integration of Linkage and Chromosome Maps of Red Clover (<b><i>Trifolium pratense</i></b> L.)

Kataoka

Hara

Kato

et al. 2012

Cytogenet Genome Res

View full text Add to dashboard Cite

Red clover (Trifolium pratense L.) is a forage legume and an allogamous diploid plant (2n = 14; 440 Mb). Here, we examine the 7 prometaphase chromosomes of red clover using fluorescence in situ hybridization (FISH) with ribosomal RNA sequences, pericentromeric and telomeric repeats, as well as bacterial artificial chromosome (BAC) clones. Position of hybridization signals and chromosome condensation patterns were quantified by the help of the chromosome image analysis system ver. 4.0 (CHIAS IV). Fourteen BAC clones belonging to linkage groups (LG) 1–7 hybridized to individual chromosomes 4, 2, 6, 5, 1, 7, and 3, respectively. Quantitative analysis of FISH mapping and chromosome analysis using CHIAS IV allowed us to construct a quantitative idiogram that constitutes the comprehensive chromosome map of red clover. Chromosomal positions of the 26S rDNA locus were detected at a heterozygous locus on chromosome 6 in the variety HR, and polymorphisms of rDNA loci were observed in other varieties, although chromosomal positions of some BAC clones did not vary among HR and other varieties. These results demonstrate chromosomal collinearity among allogamous red clover varieties. This integration of genetic linkage and quantitative chromosome maps should provide valuable insight into allogamous legume genetics.

show abstract

“…Lotus japonicus is a perennial species closely related to birdsfoot trefoil (L. corniculatus), an important forage legume. The studies performed in L. japonicus include wholegenome sequencing, molecular-marker development, and the construction of high-density linkage maps (Sato et al 2008;Ohmido et al 2010). Three linkage maps have been reported for the species, including one map based on the intraspecific cross of L. japonicus Gifu Â L. japonicus MG20 (Hayashi et al 2001;Sato and Tabata 2006), another based on the interspecific cross of L. filicaulis Â L. japonicus Gifu , and a third map under construction from the interspecific cross of L. japonicus Gifu Â L. burttii (Kawaguchi et al 2005;Sandal et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Identification of QTLs for shoot and root growth under ionic–osmotic stress in Lotus, using a RIL population

et al. 2014

View full text Add to dashboard Cite

Abstract. The genus Lotus includes a group of forage legume species including genotypes of agronomic interest and model species. In this work, an experimental hydroponic growth system allowed the discrimination of growth responses to ionic-osmotic stress in a population of recombinant inbred lines (RILs) developed from L. japonicus Â L. burttii and the identification of the associated quantitative trait loci (QTLs). The analyses led to the identification of eight QTLs: three for shoot growth localised on chromosome 3, 5 and 6; one for root growth on chromosome 1; three for total growth on chromosome 1, 4 and 5; and one associated with shoot/root ratio on chromosome 3. An interaction of QTL Â stress condition was established and the effect of the environment quantified. In summary, it was established that the allele from L. burttii explained most responses to osmotic stress, while the alleles of L. japonicus explained the responses related to ionic stress conditions. Of 49 markers linked to all QTLs identified, 41 expressed superiority of the L. burttii parental allele in the osmotic stress condition, but when an iso-osmotic concentration of NaCl was applied, L. burttii lost superiority in 21 of these markers. This shows the superiority of the L. japonicus parental allele in ionic stress conditions. This study is the first report in which a RIL population of lotus is analysed with the aim of providing molecular markers associated with plant responses to ionic or osmotic stress.

show abstract

Integration of cytogenetic and genetic linkage maps of Lotus japonicus, a model plant for legumes

Cited by 33 publications

References 41 publications

Improvements in cytological preparations for fluorescent in situ hybridization in Passiflora

Improvements in cytological preparations for fluorescent in situ hybridization in Passiflora

Integration of Linkage and Chromosome Maps of Red Clover (<b><i>Trifolium pratense</i></b> L.)

Identification of QTLs for shoot and root growth under ionic–osmotic stress in Lotus, using a RIL population

Contact Info

Product

Resources

About