Soil fertility is one of the major limiting factors for crop's productivity in Egypt and the world in general. Biological nitrogen fixation (BNF) has a great importance as a non-polluting and a cost-effective way to improve soil fertility through supplying N to different agricultural systems. Faba bean (Vicia faba L.) is one of the most efficient nitrogen-fixing legumes that can meet all of their N needs through BNF. Therefore, understanding the impact of rhizobial inoculation and contrasting soil rhizobia on nodulation and N 2 fixation in faba bean is crucial to optimize the crop yield, particularly under low fertility soil conditions. This study investigated the symbiotic effectiveness of 17 Rhizobium/Agrobacterium strains previously isolated from different Egyptian governorates in improving the nodulation and N 2 fixation in faba bean cv. Giza 843 under controlled greenhouse conditions. Five strains that had a high nitrogen-fixing capacity under greenhouse conditions were subsequently tested in field trials as faba bean inoculants at Ismaillia Governorate in northeast Egypt in comparison with the chemical N-fertilization treatment (96 kg N·ha −1 ). A starter N-dose (48 kg N·ha −1 ) was applied in combination with different Rhizobium inoculants. The field experiments were established at sites without a background of inoculation under low fertility sandy soil conditions over two successive winter growing seasons, 2012/2013 and 2013/2014. Under greenhouse conditions, inoculated plants produced significantly higher nodules dry weight, plant biomass, and shoot N-uptake than non-inoculated ones. In the first season (2012/2013), inoculation of field-grown faba bean showed significant improvements in seed yield (3.73-4.36 ton·ha −1 ) and seed N-yield (138-153 Kg N·ha −1 ), which were higher than the uninoculated control (48 kg N·ha −1 ) that produced 2.97 Kg·ha −1 and 95 kg N·ha −1 , respectively. Similarly, in the second season (2013/2014), inoculation significantly improved seed yield (3.16-4.68 ton·ha −1 ) and seed N-yield (98-155 Kg N·ha −1 ) relative to the uninoculated control (48 kg N·ha −1 ), which recorded 2.58 Kg·ha −1 and 80 kg N·ha −1 , respectively. Interestingly, faba bean inoculated with strain Rlv NGB-FR 126 showed significant increments in seed yield (35%-48%) and seed N-yield (34%-49%) compared to the inorganic N fertilizers treatment (96 kg N·ha −1 ) over the two cropping seasons, respectively. These results indicate that inoculation of faba bean with effective rhizobial strains can reduce the need for inorganic N fertilization to achieve higher crop yield under low fertility soil conditions.