2002
DOI: 10.1073/pnas.042263399
|View full text |Cite
|
Sign up to set email alerts
|

Integration of foreign DNA during natural transformation of Acinetobacter sp. by homology-facilitated illegitimate recombination

Abstract: The active uptake of extracellular DNA and its genomic integration is termed natural transformation and constitutes a major horizontal gene-transfer mechanism in prokaryotes. Chromosomal DNA transferred within a species can be integrated effectively by homologous recombination, whereas foreign DNA with low or no sequence homology would rely on illegitimate recombination events, which are rare. By using the nptII ؉ gene (kanamycin resistance) as selectable marker, we found that the integration of foreign DNA in… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1

Citation Types

14
204
1

Year Published

2004
2004
2023
2023

Publication Types

Select...
7
2

Relationship

1
8

Authors

Journals

citations
Cited by 190 publications
(219 citation statements)
references
References 69 publications
14
204
1
Order By: Relevance
“…This homologous recombination would lead to the replacement of the genes in such plasmids between the two recombination sites by the nptII gene cassette as present in the DNA of cotton MON 15985 and, thus, the acquisition of novel genetic information. The stabilisation rate of the nptII gene cassette in such bacteria is estimated from laboratory experiments with comparable constructs to be increased about 10 9 -10 10 times compared with stabilisation by the process of illegitimate recombination encountered for constructs in which no flanking homology to bacterial sequences has been introduced (De Vries and Wackernagel, 2002;Hülter and Wackernagel, 2008).…”
Section: Hazard Identification and Characterisationmentioning
confidence: 99%
“…This homologous recombination would lead to the replacement of the genes in such plasmids between the two recombination sites by the nptII gene cassette as present in the DNA of cotton MON 15985 and, thus, the acquisition of novel genetic information. The stabilisation rate of the nptII gene cassette in such bacteria is estimated from laboratory experiments with comparable constructs to be increased about 10 9 -10 10 times compared with stabilisation by the process of illegitimate recombination encountered for constructs in which no flanking homology to bacterial sequences has been introduced (De Vries and Wackernagel, 2002;Hülter and Wackernagel, 2008).…”
Section: Hazard Identification and Characterisationmentioning
confidence: 99%
“…The feasibility of short DNA molecules acting as primers in replication (Fig. 3A) is provided by trimming of the 5′-end of the incoming DNA during uptake/recombination (this study) and by cleavage of the 3′-end during uptake (33). Double-Nucleotide Variations Escaped DNA Mismatch Repair.…”
Section: Ancient Dna Can Be Taken Up and Integrated Into The A Baylyimentioning
confidence: 99%
“…4 (In fact, the distinction might not be so clear. There is mounting evidence that homologous recombination is often involved in gene addition and loss [de Vries and Wackernagel 2002;Mell et al 2011;Cordero et al 2012a;Croucher et al 2012].) Importantly, the rates and bounds of this gene transfer can vary considerably.…”
mentioning
confidence: 99%