Integration of humoral and cellular HLA-specific immune responses in cord blood allograft rejection

Hanajiri, Ryo; Murata, Makoto; Sugimoto, Kyoko; Murase, Masaki; Sakemura, Reona; Goto, Tatsunori; Watanabe, Keisuke; Imahashi, Nobuhiko; Terakura, Seitaro; Ohashi, Haruhiko; Akatsuka, Yoshiki; Kurahashi, Shingo; Miyamura, Koichi; Kiyoi, Hitoshi; Nishida, Tetsuya; Naoe, Tomoki

doi:10.1038/bmt.2015.119

Cited by 11 publications

(6 citation statements)

References 51 publications

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“…TCR-b deep sequencing was performed as described previously [14,15], with some modifications, using an Illumina MiSeq sequencer (Illumina, San Diego, CA) instead of a Roche 454 sequencer (Roche, Basel, Switzerland). In brief, cDNA was generated from total RNA using a SMARTer RACE 5 0 /3 0 kit (Takara Bio, Kusatsu, Japan).…”

Section: Tcr-b Deep Sequencingmentioning

confidence: 99%

Quantitative Assessment of T Cell Clonotypes in Human Acute Graft-versus-Host Disease Tissues

Koyama

Murata

Hanajiri

et al. 2019

Biology of Blood and Marrow Transplantation

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Owing to the difficulty in isolating T cells from human biopsy samples, the characteristics of T cells that are infiltrating human acute graft-versus-host disease (GVHD) tissues remain largely uninvestigated. In the present study, TCR-b deep sequencing of various GVHD tissue samples and concurrent peripheral blood obtained from transplant recipients was performed in combination with functional assays of tissue-infiltrating T cell clones. The T cell repertoire was more skewed in GVHD tissues than in the peripheral blood. The frequent clonotypes differed from tissue to tissue in the same patient, and the frequent clonotypes in the same tissue differed from patient to patient. Two T cell clones were successfully isolated from GVHD skin of a patient. In a cytotoxicity assay, both T cell clones lysed patient peripheral blood mononuclear cells, but not donor-derived Epstein-Barr virustransformed lymphoblastoid cells. Their clonotypes were identical to the most and second most frequent T cell clonotypes in the original GVHD skin and accounted for almost all of the skin-infiltrating T cells. These results suggest that human acute GVHD may result from only a few different alloreactive cytotoxic T cell clones, which differ from tissue to tissue and from patient to patient. The characterization of T cells infiltrating human GVHD tissues should be further investigated.

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Section: Tcr-b Deep Sequencingmentioning

confidence: 99%

Quantitative Assessment of T Cell Clonotypes in Human Acute Graft-versus-Host Disease Tissues

Koyama

Murata

Hanajiri

et al. 2019

Biology of Blood and Marrow Transplantation

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“…In unrelated donor HCT, DSAs cause graft failure and the presence of antibodies against HLA-DP was quite prominent, being observed in 60% of antibody-positive failures (25). In a study on data from the database of Japan Society for Hematopoietic Cell Transplantation (JSHCT), researchers have demonstrated that DSAs were associated with an increased risk of graft failure after CBT (34). In our study, we found that the presence of anti-HLA Abs does not have an impact on platelet engraftment, although the mechanisms behind this need further studies (35).…”

Section: Discussionmentioning

confidence: 99%

The impact of pre-transplant anti-HLA antibodies in transplants from HLA-identical sibling donors: a multicenter study

Wei²,

Chang

et al. 2023

Preprint

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Few studies have performed comparative analysis of the outcome of hematopoietic stem cell transplantation from HLA-identical sibling donors (ISD-HSCT) in patients with or without anti-HLA Abs. In this study we retrospectively collected data from a multicenter study to analyze the distribution and impact of the pre-existing anti-HLA Abs in ISD-HSCT. Among 402 recipients, 111 were tested with anti-HLA Abs. Gender, time from diagnosis to transplantation and distribution of primary disease might be risk factors for the occurrence of anti-HLA Abs. We found that patients with anti-HLA Abs had delayed neutrophil engraftment and were more vulnerable to experience Cytomegalovirus (CMV) reactivation. The presence of anti-HLA Abs was proved to be an independent risk factor for neutrophil engraftment (HR 1.42 95%CI 1.13-1.80, P=0.003) and CMV reactivation (HR 2.03 95%CI 1.19-3.46, P=0.009). We found that anti-HLA Abs have a negative impact on the prognosis in the early period after transplantation from sibling donors and anti-HLA Abs was also an independent risk factor for the overall survival (OS) at 180 days (HR 2.32, 95%CI 1.03-5.27, P=0.042) among female recipients. In conclusion, anti-HLA Abs have a negative impact on the prognosis early after ISD-HSCT.

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“…In unrelated donor HCT, DSAs cause graft failure and the presence of antibodies against HLA‐DP was quite prominent, being observed in 60% of antibody‐positive failures 32 . In a study on data from the database of Japan Society for Hematopoietic Cell Transplantation (JSHCT), researchers have demonstrated that DSAs were associated with an increased risk of graft failure after CBT 40 . In our study, we found that the presence of anti‐HLA Abs does not have an impact on platelet engraftment, although the mechanisms behind this need further studies 41 …”

Section: Discussionmentioning

confidence: 99%

The impact of pre‐transplant anti‐HLA antibodies in transplants from HLA‐identical sibling donors: A multicenter study

Wei,

Chang,

Zhu

et al. 2023

HLA

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Few studies have performed comparative analysis of the outcome of hematopoietic stem cell transplantation from HLA‐identical sibling donors (ISD‐HSCT) in patients with or without anti‐HLA Abs. In this study we retrospectively collected data from a multicenter study to analyze the distribution and impact of the pre‐existing anti‐HLA Abs in ISD‐HSCT. Among 402 recipients, 111 were positive for anti‐HLA Abs. Gender, time from diagnosis to transplantation and distribution of primary disease might be risk factors for the occurrence of anti‐HLA Abs. We found that patients with anti‐HLA Abs had delayed neutrophil engraftment and were more vulnerable to experience Cytomegalovirus (CMV) reactivation. The presence of anti‐HLA Abs was proved to be an independent risk factor for neutrophil engraftment (HR 1.42 95% CI 1.13–1.80, p = 0.003) and CMV reactivation (HR 2.03 95% CI 1.19–3.46, p = 0.009). We found that anti‐HLA Abs have a negative impact on the prognosis in the early period after transplantation from sibling donors and anti‐HLA Abs was also an independent risk factor for the overall survival (OS) at 180 days (HR 2.32, 95% CI 1.03–5.27, p = 0.042) among female recipients. In conclusion, anti‐HLA Abs have a negative impact on the prognosis early after ISD‐HSCT.

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Integration of humoral and cellular HLA-specific immune responses in cord blood allograft rejection

Cited by 11 publications

References 51 publications

Quantitative Assessment of T Cell Clonotypes in Human Acute Graft-versus-Host Disease Tissues

Quantitative Assessment of T Cell Clonotypes in Human Acute Graft-versus-Host Disease Tissues

The impact of pre-transplant anti-HLA antibodies in transplants from HLA-identical sibling donors: a multicenter study

The impact of pre‐transplant anti‐HLA antibodies in transplants from HLA‐identical sibling donors: A multicenter study

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