Integration of<i>erm</i>(B)-containing elements through large chromosome fragment exchange in<i>Clostridium difficile</i>

Wasels, François; Spigaglia, Patrizia; Barbanti, Fabrizio; Monot, Marc; Villa, Laura; Dupuy, Bruno; Carattoli, Alessandra; Mastrantonio, Paola

doi:10.1080/2159256x.2015.1006111

Cited by 8 publications

(6 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Likewise deletion of oriT reduces plasmid transfer frequencies but does not stop it; low frequency plasmid transfer to CD37 becomes completely sensitive to DNase. A similar DNase sensitive transfer of DNA has been observed when C. difficile donors containing Tn6194 were mixed with a recipient strain (Wasels et al, 2015). In this experiment cell-cell contact was required and free DNA did not result in transfer of Tn6194 (Wasels et al, 2015).…”

Section: Discussionsupporting

confidence: 67%

“…A similar DNase sensitive transfer of DNA has been observed when C. difficile donors containing Tn6194 were mixed with a recipient strain (Wasels et al, 2015). In this experiment cell-cell contact was required and free DNA did not result in transfer of Tn6194 (Wasels et al, 2015). The role of the oriT in transfer in these experiments was not investigated.…”

Section: Discussionmentioning

confidence: 72%

See 1 more Smart Citation

Plasmids can transfer to Clostridium difficile CD37 and 630Δerm both by a DNase resistant conjugation-like mechanism and a DNase sensitive mechanism

Khodadoost

Hussain

Mullany

2017

FEMS Microbiology Letters

View full text Add to dashboard Cite

Broad host range conjugative plasmids that replicate in Escherichia coli have been widely used to mobilise smaller replicons, bearing their cognate origin of transfer (oriT) into a variety of organisms that are less tractable genetically, such as Clostridium (Clostridioides) difficile. In this work we demonstrated that the oriT region of pMTL9301 (derived from RK2) is not required for transfer between E. coli and C. difficile strains 630Δerm and CD37 and that this oriT-independent transfer is abolished in the presence of DNase when CD37 is the recipient. Transfer to the 630Δerm strain is DNase resistant even without an obvious oriT, when E. coli CA434 is used as a donor and is sensitive to DNase when E. coli HB101 is the donor.

show abstract

Section: Discussionsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 72%

Plasmids can transfer to Clostridium difficile CD37 and 630Δerm both by a DNase resistant conjugation-like mechanism and a DNase sensitive mechanism

Khodadoost

Hussain

Mullany

2017

FEMS Microbiology Letters

View full text Add to dashboard Cite

show abstract

“…The element could integrate into the recipient chromosome either by homologous recombination or by using a site-specific recombinase of the recipient. Another possibility is that a portion of the donor genome containing Tn5398 is transferred to the recipient and integrates by homologous recombination [Wasels et al 2015b Tn6215 is a peculiar element conferring resistance to MLS recently characterized in C. difficile CD80 [Goh et al 2013]. Interestingly, this mobilizable transposon of about 13 kb in length can be transferred between C. difficile strains by a conjugation-like mechanism and by phage ÈC2 transduction.…”

Section: Cd630_03440mentioning

confidence: 99%

“…Interestingly, this mobilizable transposon of about 13 kb in length can be transferred between C. difficile strains by a conjugation-like mechanism and by phage ÈC2 transduction. Furthermore, very recent data suggest that both Tn6215 and Tn5398 can be transferred to the C. difficile recipient strain C. difficile CD13 by a transformation-like mechanism [Wasels et al 2015b].…”

Section: Cd630_03440mentioning

confidence: 99%

Recent advances in the understanding of antibiotic resistance in Clostridium difficile infection

Spigaglia¹

2015

Therapeutic Advances in Infection

Self Cite

226

275

View full text Add to dashboard Cite

Clostridium difficile epidemiology has changed in recent years, with the emergence of highly virulent types associated with severe infections, high rates of recurrences and mortality. Antibiotic resistance plays an important role in driving these epidemiological changes and the emergence of new types. While clindamycin resistance was driving historical endemic types, new types are associated with resistance to fluoroquinolones. Furthermore, resistance to multiple antibiotics is a common feature of the newly emergent strains and, in general, of many epidemic isolates. A reduced susceptibility to antibiotics used for C. difficile infection (CDI) treatment, in particular to metronidazole, has recently been described in several studies. Furthermore, an increased number of strains show resistance to rifamycins, used for the treatment of relapsing CDI. Several mechanisms of resistance have been identified in C. difficile, including acquisition of genetic elements and alterations of the antibiotic target sites. The C. difficile genome contains a plethora of mobile genetic elements, many of them involved in antibiotic resistance. Transfer of genetic elements among C. difficile strains or between C. difficile and other bacterial species can occur through different mechanisms that facilitate their spread. Investigations of the fitness cost in C. difficile indicate that both genetic elements and mutations in the molecular targets of antibiotics can be maintained regardless of the burden imposed on fitness, suggesting that resistances may persist in the C. difficile population also in absence of antibiotic selective pressure. The rapid evolution of antibiotic resistance and its composite nature complicate strategies in the treatment and prevention of CDI. The rapid identification of new phenotypic and genotypic traits, the implementation of effective antimicrobial stewardship and infection control programs, and the development of alternative therapies are needed to prevent and contain the spread of resistance and to ensure an efficacious therapy for CDI.

show abstract

“…Tn 6215 is another mobilizable transposon, with a length of 13 kb. It has been found to be transferred among C. difficile strains by a conjugation-like mechanism or by phage ΦC2 transduction [ 29 , 30 ]. Finally, Tn 6189 , described for the first time in 2019, a carrier of erm (B) gene, was found in a collection of both non-toxigenic and toxigenic C. difficile isolates recovered in Southeast Asia, supporting the idea of intraspecies horizontal transfer [ 31 ].…”

Section: The Role Of the Mobile Genetic Elements Of Clostridioides Difficile In The Acquisition And Transmission Of Anmentioning

confidence: 99%

Clostridioides difficile as a Dynamic Vehicle for the Dissemination of Antimicrobial-Resistance Determinants: Review and In Silico Analysis

et al. 2021

View full text Add to dashboard Cite

The present paper is divided into two parts. The first part focuses on the role of Clostridioides difficile in the accumulation of genes associated with antimicrobial resistance and then the transmission of them to other pathogenic bacteria occupying the same human intestinal niche. The second part describes an in silico analysis of the genomes of C. difficile available in GenBank, with regard to the presence of mobile genetic elements and antimicrobial resistance genes. The diversity of the C. difficile genome is discussed, and the current status of resistance of the organisms to various antimicrobial agents is reviewed. The role of transposons associated with antimicrobial resistance is appraised; the importance of plasmids associated with antimicrobial resistance is discussed, and the significance of bacteriophages as a potential shuttle for antimicrobial resistance genes is presented. In the in silico study, 1101 C. difficile genomes were found to harbor mobile genetic elements; Tn6009, Tn6105, CTn7 and Tn6192, Tn6194 and IS256 were the ones more frequently identified. The genes most commonly harbored therein were: ermB, blaCDD, vanT, vanR, vanG and vanS. Tn6194 was likely associated with resistance to erythromycin, Tn6192 and CTn7 with resistance to the β-lactams and vancomycin, IS256 with resistance to aminoglycoside and Tn6105 to vancomycin.

show abstract

Integration oferm(B)-containing elements through large chromosome fragment exchange inClostridium difficile

Cited by 8 publications

References 18 publications

Plasmids can transfer to Clostridium difficile CD37 and 630Δerm both by a DNase resistant conjugation-like mechanism and a DNase sensitive mechanism

Plasmids can transfer to Clostridium difficile CD37 and 630Δerm both by a DNase resistant conjugation-like mechanism and a DNase sensitive mechanism

Recent advances in the understanding of antibiotic resistance in Clostridium difficile infection

Clostridioides difficile as a Dynamic Vehicle for the Dissemination of Antimicrobial-Resistance Determinants: Review and In Silico Analysis

Contact Info

Product

Resources

About