Integration of metagenomic and metabolomic insights into the effects of microcystin-LR on intestinal microbiota of Litopenaeus vannamei

Abstract

Microcystin-LR (MC-LR) is a hazardous substance that threaten the health of aquatic animals. Intestinal microbes and their metabolites can interact with hosts to influence physiological homeostasis. In this study, the shrimp Litopenaeus vannamei were exposed to 1.0 μg/l MC-LR for 72 h, and the toxic effects of MC-LR on the intestinal microbial metagenomic and metabolomic responses of the shrimp were investigated. The results showed that MC-LR stress altered the gene functions of intestinal microbial, including ABC transporter, sulfur metabolism and riboflavin (VB2) metabolism, and induced a significant increase of eight carbohydrate metabolism enzymes. Alternatively, intestinal metabolic phenotypes were also altered, especially ABC transporters, protein digestion and absorption, and the biosynthesis and metabolism of amino acid. Furthermore, based on the integration of intestinal microbial metagenomic and metabolome, four bacteria species (Demequina globuliformis, Demequina sp. NBRC 110055, Sphingomonas taxi and Sphingomonas sp. RIT328) and three metabolites (yangonin, α-hederin and soyasaponin ii) biomarkers were identified. Overall, our study provides new insights into the effects of MC-LR on the intestinal microbial functions of L. vannamei.

Exposure to Microcystin-LR Promotes Colorectal Cancer Progression by Altering Gut Microbiota and Associated Metabolites in APCmin/+ Mice

“…We confirmed a significant increase in the amounts of BAs in the intestinal contents of the APC min/+ mice exposed to MC-LR. Previous studies have shown that reduced concentrations of taurocholate and taurodeoxycholic acid, coupled with increased taurine levels, suggest that MC-LR could influence bile acid metabolic processes [64]. Subsequently, we also noted that the metabolite changes were significantly related to the changes in gut microbial abundance, with TCA having the most positive correlation with Marvinbryantia and Family_XIII_AD3011_group.…”

Integration of Multi-Omics, Histological, and Biochemical Analysis Reveals the Toxic Responses of Nile Tilapia Liver to Chronic Microcystin-LR Exposure

“…In line with these results, a previous study reported that acute exposure to MC-LR lowered the relative expression level of cyp7a1 in the liver of Chinese mitten crab ( Hypophthalmicthys molitrix ) [ 36 ]. Another study on shrimp showed that MC-LR exposure decreased the intestinal levels of taurocholate and taurodeoxycholic acid, affecting the bile acid metabolism of shrimp [ 37 ]. Moreover, consistent with the transcriptomic results, metabolic results showed that BA synthesis raw material (taurine) and primary BA (cholic acid and taurocholic acid) levels in the Nile tilapia liver decreased after exposure to MC-LR.…”